Multi-scale Fabrication Techniques of Collagen Hydrogel for Developing Physiological 3D In vitro Barrier Model

2022 ◽  
Author(s):  
Hyeonjun Hong ◽  
Seongsu Eom ◽  
Seong Jin Lee ◽  
Jaeseung Youn ◽  
Dohui Kim ◽  
...  
Keyword(s):  
Multi Scale ◽  
Collagen Hydrogel ◽  
Barrier Model

Understanding the multi-scale structure, physicochemical properties and in vitro digestibility of citrate naked barley starch induced by non-thermal plasma

2021 ◽  
Author(s):  
Huishan Shen ◽  
Xiangzhen Ge ◽  
Bo Zhang ◽  
Chunyan Su ◽  
Qian Zhang ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Thermal Plasma ◽  
Scale Structure ◽  
In Vitro Digestibility ◽  
Multi Scale ◽  
Naked Barley ◽  
Starch Modification ◽  
Plasma Pretreatment ◽  
Non Thermal Plasma

Non-thermal plasma is an emerging and effective starch modification technology. In this paper, plasma pretreatment was used to modify the citrate naked barley starch for enhancing the accessibility of citric...

scholarly journals Magnetic hybrid materials interact with biological matrices

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Christine Gräfe ◽  
Elena K. Müller ◽  
Lennart Gresing ◽  
Andreas Weidner ◽  
Patricia Radon ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Field Gradient ◽  
Hybrid Materials ◽  
Biomedical Application ◽  
Magnetic Field Gradient ◽  
Apical Cell ◽  
Cell Type ◽  
Cell Layers ◽  
Barrier Model

Abstract Magnetic hybrid materials are a promising group of substances. Their interaction with matrices is challenging with regard to the underlying physical and chemical mechanisms. But thinking matrices as biological membranes or even structured cell layers they become interesting with regard to potential biomedical applications. Therefore, we established in vitro blood-organ barrier models to study the interaction and processing of superparamagnetic iron oxide nanoparticles (SPIONs) with these cellular structures in the presence of a magnetic field gradient. A one-cell-type–based blood-brain barrier model was used to investigate the attachment and uptake mechanisms of differentially charged magnetic hybrid materials. Inhibition of clathrin-dependent endocytosis and F-actin depolymerization led to a dramatic reduction of cellular uptake. Furthermore, the subsequent transportation of SPIONs through the barrier and the ability to detect these particles was of interest. Negatively charged SPIONs could be detected behind the barrier as well as in a reporter cell line. These observations could be confirmed with a two-cell-type–based blood-placenta barrier model. While positively charged SPIONs heavily interact with the apical cell layer, neutrally charged SPIONs showed a retarded interaction behavior. Behind the blood-placenta barrier, negatively charged SPIONs could be clearly detected. Finally, the transfer of the in vitro blood-placenta model in a microfluidic biochip allows the integration of shear stress into the system. Even without particle accumulation in a magnetic field gradient, the negatively charged SPIONs were detectable behind the barrier. In conclusion, in vitro blood-organ barrier models allow the broad investigation of magnetic hybrid materials with regard to biocompatibility, cell interaction, and transfer through cell layers on their way to biomedical application.

Biomanufacturing of a novel in vitro biomimetic blood-brain barrier model

2020 ◽  
Vol 12 (3) ◽  
pp. 035008
Author(s):  
Libiao Liu ◽  
Xinda Li ◽  
Xinzhi Zhang ◽  
Tao Xu
Keyword(s):  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Blood Brain ◽  
Blood Brain Barrier Model ◽  
Barrier Model

scholarly journals Implementation of a dynamic intestinal gut-on-a-chip barrier model for transport studies of lipophilic dioxin congeners

RSC Advances ◽  
2018 ◽  
Vol 8 (57) ◽  
pp. 32440-32453 ◽  
Author(s):  
Kornphimol Kulthong ◽  
Loes Duivenvoorde ◽  
Barbara Z. Mizera ◽  
Deborah Rijkers ◽  
Guillaume ten Dam ◽  
...  
Keyword(s):  
Great Promise ◽  
Transport Studies ◽  
Functional Complexity ◽  
Organ On A Chip ◽  
Barrier Model

Novel microfluidic technologies allow the manufacture ofin vitroorgan-on-a-chip systems that hold great promise to adequately recapitulate the biophysical and functional complexity of organs foundin vivo.

scholarly journals Role of matrix metalloproteinase-2/9 (MMP2/9) in lead-induced changes in an in vitro blood-brain barrier model

2017 ◽  
Vol 13 (11) ◽  
pp. 1351-1360 ◽  
Author(s):  
Xinqin Liu ◽  
Peng Su ◽  
Shanshan Meng ◽  
Michael Aschner ◽  
Yupeng Cao ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Matrix Metalloproteinase 2 ◽  
Blood Brain ◽  
Blood Brain Barrier Model ◽  
Induced Changes ◽  
Barrier Model
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