Development and characterization of ten microsatellite markers for population studies of the native Brazilian oyster Crassostrea gasar

2012 ◽  
Vol 4 (3) ◽  
pp. 583-586 ◽  
Author(s):  
Mauro André Damasceno Melo ◽  
Adam Rick Bessa da Silva ◽  
Eduardo Sousa Varela ◽  
Iracilda Sampaio ◽  
Claudia Helena Tagliaro
Keyword(s):  
Microsatellite Markers ◽  
Population Studies

Isolation and characterization of 14 microsatellite markers for Rhizophora mucronata (Rhizophoraceae) and their potential use in range-wide population studies

2012 ◽  
Vol 4 (4) ◽  
pp. 951-954 ◽  
Author(s):  
Yoshimi Shinmura ◽  
Alison K. S. Wee ◽  
Koji Takayama ◽  
Sankararamasubramanian Halasya Meenakshisundaram ◽  
Takeshi Asakawa ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Population Studies ◽  
Rhizophora Mucronata ◽  
Isolation And Characterization ◽  
Potential Use ◽  
Wide Population

scholarly journals Transferability and Characterization of Microsatellite Markers from Byrsonima Cydoniifolia A. Juss. (MALPIGHIACEAE) in Seven Related Taxa from Cerrado Biome Reveal Genetic Relationships

2021 ◽  
Author(s):  
Vanessa Bernardes ◽  
Devanir M. Murakami ◽  
Nair Bizão ◽  
Tamara N. Souza ◽  
Marcos J. da Silva ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Genetic Divergence ◽  
High Efficiency ◽  
Population Studies ◽  
Genetic Relationships ◽  
Species Occurrence ◽  
Genetic Population ◽  
Expected Heterozygosity

Abstract Byrsonima Rich. is one of the largest genera of the Malpighiaceae family, with 97 species occurrence in Brazil. In this study, 17 microsatellite markers previously developed and characterized in Byrsonima cydoniifolia A. Juss. were tested for seven related taxa. All species tested here are native to Brazil, and of these species four are endemic. Cross-amplification as successfully optimized with high efficiency for all species. Microsatellite markers panels ranged from 11 (64,8%) transfered markers in B. viminifolia to 6 (35.2%) in B. umbellata. All loci were genotyped for 16 individuals of each species, except for B. viminifolia (14 individuals), then the polymorphic loci were characterized. The total number of alleles per locus across tested species ranged from 5 (B. linearifolia) to 8 (B. subterranea) alleles. B. umbellata showed lower values of observed and expected heterozygosity (Ho = 0.312; He = 0.436) and B. subterranea presented the highest values (Ho = 0.687; He = 0.778). A greater number of microsatellite markers should be developed for B. umbellata, once that the markers set transferred is reasonably informative, due to the greatest genetic divergence between species. The microsatellite marker panels transferred to the species B. intermedia, B. verbascifolia, B. laxiflora, B. subterranea, B. viminifolia and B. linearifolia are very informative, with a high combined probability of exclusion of paternity (Q ≥ 0.976) and the low combined probability of identity (I ≤ 9.91x10− 6), potentially suitable for future genetic-population studies, supporting strategies for maintaining the genetic diversity and for exploration of Byrsonima species as genetic resources.

Development and characterization of fungal specific microsatellite markers in the lichenLobarina scrobiculata(Lobariaceae, Ascomycota)

2015 ◽  
Vol 47 (3) ◽  
pp. 183-186 ◽  
Author(s):  
Maria Prieto ◽  
Lidia Romera ◽  
Sonia Merinero ◽  
Gregorio Aragón ◽  
Isabel Martínez
Keyword(s):  
Microsatellite Markers ◽  
Iberian Peninsula ◽  
Population Studies ◽  
Gene Diversity ◽  
Conservation Status ◽  
Natural Populations ◽  
Conservation Strategies ◽  
Unbiased Gene ◽  
Effective Conservation

AbstractLobarina scrobiculata(better known asLobaria scrobiculata) is a widespread lichen, threatened and Red-Listed in various European countries. Microsatellite markers for the mycobiont ofL. scrobiculatawere developed in order to investigate its genetic diversity in the Iberian Peninsula and Europe and to design effective conservation strategies. A total of 7 polymorphic markers were isolated and characterized. These microsatellites were tested in natural populations found in the Iberian Peninsula. The number of observed alleles ranged from 3 to 8, and the Nei's unbiased gene diversity from 0·26 to 0·59. These microsatellite markers are the first to be developed forL. scrobiculataand they will be useful for population studies and for the assessment of the conservation status of this species.

Screening of polymorphic microsatellite markers from the whole-genome sequences of nipa (Nypa fruticans Wurmb.)

2021 ◽  
pp. 1-4
Author(s):  
Junaldo A. Mantiquilla ◽  
Merlene E. Elumba ◽  
Meng-Shin Shiao ◽  
Yu-Chung Chiang
Keyword(s):  
Microsatellite Markers ◽  
Population Studies ◽  
Population Level ◽  
Whole Genome ◽  
Sequencing Technology ◽  
Evolutionary Forces ◽  
Nypa Fruticans ◽  
Polymorphic Microsatellite ◽  
Simple Sequence

Abstract Nipa (Nypa fruticans Wurmb.), aside from being part of the mangrove forest ecosystem that has been under assault at an alarming rate, lacks information on its genetic variation at the population level for conservation planning purposes. Microsatellites, or simple sequence repeats (SSRs), are molecular markers employed to detect genetic variations in population studies. Here, the primers for this palm were identified through whole-genome sequencing using the Illumina® Sequencing Technology platform. Over half (52.4%) of SSRs consisted of mononucleotide repeats, whereas the remainder mostly consisted of dinucleotide (22.7%) and trinucleotide (22.4%) repeats. The frequencies of longer repeats (tetranucleotides, pentanucleotides and hexanucleotides) were exceptionally rare (2.5%). At least 18 out of 75 genomic loci were polymorphic, based on 37 randomly selected samples assayed for screening. Polymorphic loci (P) reached a mean of 72% across the Indo-West Pacific region. Estimates of genetic diversity showed a lower mean observed heterozygosity (Ho) (0.195) than expected (He) (0.316), suggesting evolutionary forces were acting across nipa populations. The fixation (F) index (0.388) supported this result, which indicates considerable genetic differentiation among populations in the region. An average of 2.111 alleles (Na) was obtained. In conclusion, these microsatellite markers are promising tools for genetic evaluation of nipa populations to facilitate characterization of this species in its distribution range.

Development and characterization of 24 microsatellite markers in Primula tosaensis , an endangered primrose, using MiSeq

2017 ◽  
Vol 33 (1) ◽  
pp. 77-80 ◽  
Author(s):  
Masaya Yamamoto ◽  
Yoshihiro Handa ◽  
Hiroki Aihara ◽  
Hiroaki Setoguchi
Keyword(s):  
Microsatellite Markers

Genetic characterization of novel polymorphic microsatellite markers for Epilobium nankotaizanense (Onagraceae), an endemic and threatened herb in Taiwan

2021 ◽  
pp. 1-4
Author(s):  
Yu-Wei Tseng ◽  
Chi-Chun Huang ◽  
Chih-Chiang Wang ◽  
Chiuan-Yu Li ◽  
Kuo-Hsiang Hung
Keyword(s):  
Microsatellite Markers ◽  
Natural Populations ◽  
Conservation Strategy ◽  
Sequencing Data ◽  
Germplasm Collections ◽  
The Family ◽  
Conservation Genetic ◽  
Population Sizes ◽  
Polymorphic Microsatellite

Abstract Epilobium belongs to the family Onagraceae, which consists of approximately 200 species distributed worldwide, and some species have been used as medicinal plants. Epilobium nankotaizanense is an endemic and endangered herb that grows in the high mountains in Taiwan at an elevation of more than 3300 m. Alpine herbs are severely threatened by climate change, which leads to a reduction in their habitats and population sizes. However, only a few studies have addressed genetic diversity and population genetics. In the present study, we developed a new set of microsatellite markers for E. nankotaizanense using high-throughput genome sequencing data. Twenty polymorphic microsatellite markers were developed and tested on 30 individuals collected from three natural populations. These loci were successfully amplified, and polymorphisms were observed in E. nankotaizanense. The number of alleles per locus (A) ranged from 2.000 to 3.000, and the observed (Ho) and expected (He) heterozygosities ranged from 0.000 to 0.929 and from 0.034 to 0.631, respectively. The developed polymorphic microsatellite markers will be useful in future conservation genetic studies of E. nankotaizanense as well as for developing an effective conservation strategy for this species and facilitating germplasm collections and sustainable utilization of other Epilobium species.

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