scholarly journals Ex situ conservation of Cymbidium eburneum Lindl.: a threatened and vulnerable orchid, by asymbiotic seed germination

3 Biotech ◽  
2012 ◽  
Vol 2 (4) ◽  
pp. 337-343 ◽  
Author(s):  
Kiran Gogoi ◽  
Suman Kumaria ◽  
Pramod Tandon
Keyword(s):  
Seed Germination ◽  
Ex Situ Conservation ◽  
Ex Situ ◽  
Asymbiotic Seed Germination

scholarly journals The reproductive biology of Calligonum L. in relation to ex situ conservation in a botanical garden

2011 ◽  
Vol 63 (3) ◽  
pp. 799-809 ◽  
Author(s):  
Kang Xiaoshan ◽  
Pan Borong ◽  
Duan Shimin ◽  
Shi Wei ◽  
Zhang Yongzhi
Keyword(s):  
Seed Germination ◽  
Ex Situ Conservation ◽  
Flowering Phenology ◽  
Botanical Garden ◽  
Breeding Systems ◽  
Ex Situ ◽  
Germplasm Resources ◽  
Botanic Garden ◽  
Theoretical Support ◽  
Germplasm Resource

In this study, we observed the flowering phenology, breeding system, pollination and seed germination of four species of Calligonum (C. calliphysa, C. rubicundum, C. densum and C. ebinuricum) in the Turpan Eremophytes Botanic Garden, China. Our results showed that the species had overlapping flowering phenologies and were pollinated by similar pollination agents. Their breeding systems were self-compatible, and with signs of outbreeding, but not of hybridization with each other; the main isolation mechanism was post-zygotic isolation and they also had high seed germination rates. Therefore, they are suited to ex situ conservation in the Turpan Eremophytes Botanic Garden, and can supply sufficient seeds for renewal populations and the conservation of germplasm resources. Furthermore, these results provide theoretical support for the construction of a national germplasm resource garden of Calligonum, and for the introduction to the garden of other eremophyteplants and their conservation.

scholarly journals Asymbiotic seed germination and multiplication of an endangered orchid – Paphiopedilum venustum (Wall. ex Sims.)

2016 ◽  
Vol 85 (2) ◽  
Author(s):  
Saranjeet Kaur ◽  
Kamlesh Kumar Bhutani
Keyword(s):  
Seed Germination ◽  
Shoot Multiplication ◽  
Ex Situ ◽  
Terrestrial Orchid ◽  
Asymbiotic Seed Germination ◽  
Orchid Seed ◽  
Early Seedling ◽  
Potato Powder ◽  
Survival Frequency

The present study was intended to facilitate ex situ conservation of <em>Paphiopedilum venustum</em>, a highly floriferous endangered terrestrial orchid species. A protocol was established for in vitro propagation and shoot multiplication. The cultures were initiated through asymbiotic seed germination technique, using undehisced and dehisced capsules. Four defined asymbiotic orchid seed germination media (terrestrial orchid medium, modified terrestrial orchid medium, Malmgren modified terrestrial orchid medium, Knudson C medium) were evaluated for their effectiveness in achieving maximum seed germination and early seedling development. The effect of darkness and 12-h photoperiod was also tested. Optimum seed germination, i.e., 82.7% was achieved on modified terrestrial orchid medium under a 12-h photoperiod using seeds from undehisced capsules. Shoot multiplication was accomplished using organic [peptone (1.0, 2.0 g L<sup>−1</sup>)] and inorganic [banana homogenate (10, 20, 30 g L<sup>−1</sup>) and potato powder (5.0, 10 g L<sup>−1</sup>)] growth supplements. Peptone at 1.0 g L<sup>−1</sup> was the most effective in multiplying the shoots. Plantlets were acclimatized in the greenhouse with 80% survival frequency.

scholarly journals PRESENT STATUS AND EX-SITU CONSERVATION OF MORINGA CONCANENSIS IN INDIAN DESERT

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
C.S. Purohit ◽  
R. N. Kulloli ◽  
Suresh Kumar
Keyword(s):  
Seed Germination ◽  
Present Status ◽  
Ex Situ Conservation ◽  
Ex Situ ◽  
Desert Plant ◽  
Seedling Mortality ◽  
Indian Desert ◽  
Plant Parts

Moringa concanensis Nimmo. ex Dalz. & Gibs. is rare economic important plant distributed in Indian desert. Plant parts are used for different medicinal purposes. Present study was conducted to assess its status in nature as well as different seed germination treatment for its ex-situ conservation. Study revealed that germination was 100% in coco-peat media while, it was 97.56% in polythene bags. Seedling mortality was more (39.46%) in coco-peat media. After transplantation seedlings showed 82% survival in field.

scholarly journals Seed germination of Carex lainzii Luceño, E. Rico & T. Romero: An endemic Spanish endangered species

2019 ◽  
Vol 20 (3) ◽  
pp. 704-711
Author(s):  
KAROLINE APARECIDA FELIX RIBEIRO ◽  
CRISTIAN MADEIRA DE MEDEIROS ◽  
JOSÉ ÁNGEL SÁNCHEZ-AGUDO ◽  
JOSÉ SÁNCHEZ-SÁNCHEZ
Keyword(s):  
Seed Germination ◽  
Endangered Species ◽  
Ex Situ Conservation ◽  
Seed Viability ◽  
Potassium Nitrate ◽  
Ex Situ ◽  
Cold Stratification ◽  
Different Temperatures ◽  
Two Populations

Abstract. Ribeiro KAF, Madeira de Medeiros C, Agudo JAS, Sánchez JS. 2019. Seed germination of Carex lainzii Luceño, E. Rico & T. Romero: An endemic Spanish endangered species. Biodiversitas 20: xxxx. Strategies to halt the decline of biodiversity include: in-situ and ex-situ conservation, the latter already considered at the global level essential in conservation programs. The results of the germinative responses of Carex lainzii Luceño, E. Rico & T. Romero (Cyperaceae), an endemic Spanish endangered species, are presented in this work, to different trials carried out in the laboratory with seeds harvested in the two known populations of that community. Treatments with and without 0.2% potassium nitrate (KNO3) were tested for four, six and eight months in cold stratification at 5 °C in two germination chambers at different temperatures (22/10 °C and 27/15 °C with a photoperiod of 12/12 hours). The results indicate that there is a large difference in germination rates between the two populations. The use of KNO3 did not increase germination rates in any case, but differences were found between periods of cold stratification, with six months being the most effective. On the other hand, the germination rates of both populations remain low (17.58% and 2%) compared to the obtained rate of seed viability. With this first approach to the understanding of the germination requirements of C. lainzii, it becomes clear that new essays are needed to obtain better results, in order to effectively implement the protection plans of C. lainzii populations.

Effect of temperature and light on seed germination of Erysimum naxense and Erysimum krendlii

2013 ◽  
Vol 8 (12) ◽  
pp. 1194-1203 ◽  
Author(s):  
Paraskevi Gkika ◽  
Nikos Krigas ◽  
George Menexes ◽  
Ilias Eleftherohorinos ◽  
Eleni Maloupa
Keyword(s):  
Seed Germination ◽  
Ex Situ Conservation ◽  
Red Light ◽  
Seed Viability ◽  
Ex Situ ◽  
Effect Of Temperature ◽  
Natural Habitats ◽  
Experimental Conditions ◽  
Light Regimes ◽  
Species Specific

AbstractSeed germination of two local Greek endemics was studied (Erysimum naxense, Erysimum krendlii). Seed viability was determined by using the tetrazolium method and germination was studied in synchronized cycles of five and four alternating temperatures [10/5 (for E. naxense only) and 15/10, 20/15, 25/20, and 30/25°C for both species, in cycles of 16 h day/8 h night], and in five light regimes (red, blue, green, white, and dark). Germination of E. naxense and E. krendlii seeds was determined daily for six and five weeks, respectively, with the data analyzed as viability adjusted accumulative seed germination at the end of each week. E. naxense’s seed viability was higher (90%) than that of E. krendlii (64%); seed germination (%) of both increased at low alternating temperatures (10/5°C, 15/10°C, 20/15°C). Germination of E. naxense seeds at low temperatures was light-independent, whereas at high temperatures it was increased with red light. Germination of E. krendlii seeds was inconsistently affected by light at the temperatures studied. Percentages of seed germination of both species were higher in experimental conditions similar to the ones of their natural habitats during autumn and/or spring (facilitated with Geographic Information Systems). These conclusions provide guidelines for species-specific propagation protocols and ex situ conservation.

scholarly journals Investigation of vegetative properties and generative production of the potential ornamental and narrow endemic species Verbascum yurtkuranianum (Scrophulariaceae) for ex situ conservation

BioResources ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. 7530-7549
Author(s):  
Kamil Erken
Keyword(s):  
Seed Germination ◽  
Endemic Species ◽  
Ex Situ Conservation ◽  
Germination Rate ◽  
Ultrasonic Waves ◽  
Ornamental Plant ◽  
Ex Situ ◽  
Narrow Endemic ◽  
Single Location ◽  
Number Of Individuals

Verbascum yurtkuranianum is a narrow endemic species occurring in a single location, the northern Bursa province (Turkey). It is an endangered and potentially ornamental plant. No conducted study on its life and biology, production, and aesthetic features is available. This study aimed to reveal its vegetative properties, seed characteristics, methods and requirements for seed germination, germination speed, and potential ornamental value so it can be conserved ex situ and produced. Verbascum yurtkuranianum has potential value as an ornamental plant regarding its aesthetic features as a flower. This study revealed that the total number of individuals in the species is 788. Without any treatment, 70.7% germination rate is achieved if the seeds are stored at 4 °C. The optimum germination temperature was from 15 to 20 °C (77.3% and 78.7%, respectively), and the photoperiod regulation for seed germination was 12/12 or 8/16 (light/dark) hours (74.7% and 76.0%, respectively). The most effective treatment to promote germination rate was found by implementation of 60 min ultrasonic waves (94.3%) or application of 120 min vacuum (95.3%). Germination occurred between 8 and 10 days. A parcel of ex situ conservation was constituted with the seedlings obtained from the germination studies.

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