scholarly journals Statistical optimization of cultural medium composition of thermoalkalophilic lipase produced by a chemically induced mutant strain of Bacillus atrophaeus FSHM2

3 Biotech ◽  
2019 ◽  
Vol 9 (7) ◽  
Author(s):  
Atefeh Ameri ◽  
Mojtaba Shakibaie ◽  
Zahra Sahami ◽  
Mehdi Khoobi ◽  
Hamid Forootanfar
Keyword(s):  
Mutant Strain ◽  
Medium Composition ◽  
Statistical Optimization ◽  
Bacillus Atrophaeus ◽  
Cultural Medium ◽  
Chemically Induced ◽  
Induced Mutant

scholarly journals Statistical optimization of kojic acid production by a UV-induced mutant strain of Aspergillus terreus

2018 ◽  
Vol 49 (4) ◽  
pp. 865-871 ◽  
Author(s):  
Mojtaba Shakibaie ◽  
Atefeh Ameri ◽  
Roya Ghazanfarian ◽  
Mahboubeh Adeli-Sardou ◽  
Sahar Amirpour-Rostami ◽  
...  
Keyword(s):  
Mutant Strain ◽  
Acid Production ◽  
Aspergillus Terreus ◽  
Kojic Acid ◽  
Statistical Optimization ◽  
Kojic Acid Production ◽  
Induced Mutant

Statistical optimization of medium composition for growth ofLeuconostoc citreum

2004 ◽  
Vol 9 (4) ◽  
pp. 278-284 ◽  
Author(s):  
Hyun Kim ◽  
Hyun-Ju Eom ◽  
Junsoo Lee ◽  
Jinsoo Han ◽  
Nam Soo Han
Keyword(s):  
Medium Composition ◽  
Statistical Optimization

scholarly journals Improving the monacolin K to citrinin production ratio in red yeast rice by an X-ray-induced mutant strain of Monascus purpureus

2018 ◽  
Vol 99 (2) ◽  
pp. 109-118 ◽  
Author(s):  
Apinun Kanpiengjai ◽  
Rapeepun Mahawan ◽  
Prodpran Pengnoi ◽  
Saisamorn Lumyong ◽  
Chartchai Khanongnuch
Keyword(s):  
Mutant Strain ◽  
Monascus Purpureus ◽  
Red Yeast Rice ◽  
Monacolin K ◽  
X Ray ◽  
Production Ratio ◽  
Red Yeast ◽  
Induced Mutant

scholarly journals On Enrichment Culturing and Transferring Technique

2021 ◽  
Vol 6 (1) ◽  
pp. 1-5
Author(s):  
Ji-Dong Gu ◽  
Keyword(s):  
Environmental Science ◽  
Scientific Information ◽  
Medium Composition ◽  
Isolation And Purification ◽  
Complex Samples ◽  
Fundamental Information ◽  
Cultural Medium ◽  
Wide Range ◽  
Basic Understanding ◽  
Pure Cultures

Enrichment culturing and transferring technique is frequently used to obtain specific functional microorganisms for more in-depth investigations from the complex samples containing a wide range of different microorganisms. This technique is fundamental and critically important in research of microbial ecology, environmental microbiology, and environmental science, but the proper practice of this technique in applications remains ambiguous to some investigators. Because of this situation, misuse and lack of comprehensive understanding of the meaning of this technique are frequently found in manuscripts or even publications. This article provides a discussion about this technique and the meaning for practical use to enhance research for high-quality results and the scientific information effectively when used. The key elements of this technique include, cultural medium composition, culturing and incubation, frequency of transferring for enrichment, procedures prior to the isolation and purification to obtain possibly pure cultures or enrichments of the capable microorganisms for further biochemistry and physiology investigations. The information is intended to improve the basic understanding of this technique for a more effective and efficient applications in research to advance the basic and fundamental information and to pave the way for more innovative research and discoveries to science.

scholarly journals Statistical optimization of culture medium composition for enhanced zeaxanthin production by Cyanophycean microalgae Trichodesmium thiebautii (NIOT 152)

2020 ◽  
Vol 13 (41) ◽  
pp. 4307-4318
Author(s):  
Priyanka Srinivasan ◽  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Sea Water ◽  
Fold Increase ◽  
Medium Composition ◽  
Statistical Optimization ◽  
Age Related Macular Degeneration ◽  
Medium Components ◽  
Age Related ◽  
Trichodesmium Thiebautii

Background/Objectives: Zeaxanthin is a xanthophyll carotenoid revered for its role in the prevention of age related macular degeneration. The study evaluated the zeaxanthin accumulation of the marine Cyanophycean alga Trichodesmium thiebautii (NIOT 152). A sequential statistical technique was applied to optimize the Artificial Sea Water nutrient medium (ASN-III) components for enhancing the zeaxanthin accumulation in T. thiebautii. Methods: A two-level statistical approach involving Plackett-Burman (PB) design to screen the most important nutrients influencing the zeaxanthin accumulation followed by Response surface methodology (RSM) was employed. The results of PB design revealed sodium nitrate, disodium EDTA, magnesium sulphate and sodium carbonate as the crucial medium components for increasing zeaxanthin accumulation. Further, RSM was employed to study the interaction between these factors and identified an optimum concentration of the ingredients for higher zeaxanthin production. Findings: The optimized medium components resulted in 2.33 fold increase in zeaxanthin accumulation (4.3 ± 1.29 mg L-1) as compared to ASN III medium (1.84 ± 0.12 mg L-1). Novelty: There are only few studies on laboratory cultured Trichodesmium and only very few reports are available regarding pigment production from Trichodesmium sp. The present study successfully demonstrated the statistical optimization of ASN III medium to improve zeaxanthin accumulation by Trichodesmium thiebautii. Keywords: ASN III medium; zeaxanthin; Trichodesmium thiebautii; Plackett-Burman; response surface methodology REFERENCE

Overproduction of thermoalkalophilic lipase secreted by Bacillus atrophaeus FSHM2 using UV-induced mutagenesis and statistical optimization of medium components

2019 ◽  
Vol 49 (2) ◽  
pp. 184-191 ◽  
Author(s):  
Atefeh Ameri ◽  
Mojtaba Shakibaie ◽  
Mozhde Soleimani-Kermani ◽  
Mohammad Ali Faramarzi ◽  
Mohsen Doostmohammadi ◽  
...  
Keyword(s):  
Statistical Optimization ◽  
Bacillus Atrophaeus ◽  
Medium Components ◽  
Induced Mutagenesis

scholarly journals Process optimization for amylase production of Bacillus subtilis M4 mutant strain

2019 ◽  
Vol 27 (02) ◽  
pp. 8-19
Author(s):  
Naramchimeg B ◽  
Altantsetseg Kh ◽  
Urantulkhuur B
Keyword(s):  
Bacillus Subtilis ◽  
Mutant Strain ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Fold Increase ◽  
Medium Composition ◽  
Optimization Techniques ◽  
Amylase Production ◽  
Ph Range ◽  
Inoculum Volume

There are many factors that influence the character of bacterial metabolism and enzyme production.For themaximum production of the desired products, the media components and fermentation conditions should beoptimized. In our investigation, we improved the amylase production of Bacillus subtilis M4 mutant strain bythe combination of two optimization techniques. The cultural conditions (time period, temperature, pH,inoculum volume) and medium ingredients (various carbon, organic and inorganic nitrogen sources, chlorides, sulfates, phosphates, carbonates) were optimized by one factor at a time methodology (OFAT) and response surface methodology (RSM) to increase the amylase production. The optimum conditions for amylase production were found be the following: 35ºC, pH range 7 and incubation time 72h, inoculum volume 8% (v/v). Optimum medium composition for amylase production was the following: starch 12.9 g, peptone 9.75g, calcium carbonate 0.439 g, magnesium sulfate 0.464 g and potassium chloride 0.464 g per liter. Whenapplied to our optimized medium in the fermentation process, the enzyme activity increased from 0.741 to1.58 U/ml, which means a 2.1-fold increase compared to the original medium.

Sign in / Sign up

Export Citation Format

Share Document