Karyotype analysis and report on B-chromosome in Gloriosa superba L. by differential staining

The Nucleus ◽  
2018 ◽  
Vol 62 (1) ◽  
pp. 31-38
Author(s):  
Syeda Sharmeen Sultana ◽  
Chandan Kumar Dash ◽  
Sheikh Shamimul Alam ◽  
Md. Abul Hassan
Keyword(s):  
Karyotype Analysis ◽  
B Chromosome ◽  
Differential Staining ◽  
Gloriosa Superba

scholarly journals Comparative Karyotype Analysis with Differential Staining in Two Forms of Anabas testudineus Bloch

CYTOLOGIA ◽  
2007 ◽  
Vol 72 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Shiuly Rahman Tinni ◽  
Nasrin Sultana Jessy ◽  
Mehedi Mahmudul Hasan ◽  
Md. Ghulam Mustafa ◽  
Sheikh Shamimul Alam
Keyword(s):  
Karyotype Analysis ◽  
Differential Staining ◽  
Anabas Testudineus

scholarly journals Chromosomes of parasitic wasps of the superfamily Chalcidoidea (Hymenoptera): An overview

2020 ◽  
Vol 14 (3) ◽  
pp. 399-416
Author(s):  
Vladimir E. Gokhman
Keyword(s):  
Current Knowledge ◽  
Local Maximum ◽  
B Chromosome ◽  
Differential Staining ◽  
Haploid Chromosome Number ◽  
Phylogenetic Reconstructions ◽  
Taxonomic Implications ◽  
Cryptic Taxa ◽  
Chromosomal Mutations

An overview of the current knowledge of chromosome sets of the parasitoid superfamily Chalcidoidea is given. Karyotypes of approximately 240 members of this group, i.e. just above one percent of described species, are studied up to now. Techniques for obtaining and analyzing preparations of chalcid chromosomes are outlined, including the so-called “traditional” and “modern” methods of differential staining as well as fluorescence in situ hybridization (FISH). Among the Chalcidoidea, the haploid chromosome number can vary from n = 3 to n = 11, with a clear mode at n = 6 and a second local maximum at n = 10. In this group, most chromosomes are either metacentric or submetacentric, but acrocentrics and/or subtelocentrics also can predominate, especially within karyotypes of certain Chalcidoidea with higher chromosome numbers. The following main types of chromosomal mutations are characteristic of chalcid karyotypes: inversions, fusions, translocations, polyploidy, aneuploidy and B chromosome variation. Although karyotype evolution of this superfamily was mainly studied using phylogenetic reconstructions based on morphological and/or molecular characters, chromosomal synapomorphies of certain groups were also revealed. Taxonomic implications of karyotypic features of the Chalcidoidea are apparently the most important at the species level, especially among cryptic taxa.

scholarly journals Cytogenetic karyotype analysis in selected species of the Erinaceidae family

2019 ◽  
Vol 63 (3) ◽  
pp. 353-358
Author(s):  
Anna Grzesiakowska ◽  
Przemysław Baran ◽  
Marta Kuchta-Gładysz ◽  
Olga Szeleszczuk
Keyword(s):  
Karyotype Analysis ◽  
Chromosome Analysis ◽  
Differential Staining ◽  
C Banding ◽  
Repeat Sequences ◽  
New Information ◽  
Karyotype Structure ◽  
Staining Techniques ◽  
Erinaceus Roumanicus ◽  
Made In

Abstract Introduction Comparative analysis of the karyotype structure was made in two hedgehog species: the northern white-breasted hedgehog (Erinaceus roumanicus) and the African pygmy hedgehog (Atelerix albiventris). Material and Methods The cytogenetic analysis used differential staining techniques (DAPI, Ag-NOR, and C-banding/DAPI) and sequential QFQ/FISH banding with NOR20 and TEL20 probes which showed 45S rDNA and (TTAGGG)n repeat sequences, respectively, on hedgehog chromosomes. Results It was confirmed that the somatic cells of the hedgehogs have a constant number of chromosomes (2n = 48,XY). Differences were observed in the NOR number between the species. NORs were identified on three autosome pairs in the northern white-breasted hedgehog and on only two pairs in the African pygmy hedgehog. Chromosome analysis by C-banding/DAPI showed large segments of heterochromatin rich in A-T pairs on three autosome pairs in both the northern white-breasted and African pygmy hedgehogs. The heterochromatin segments encompassed large fragments of the longer arm of chromosome pairs 13, 14 and 20. The (TTAGGG)n repeat sequences on the hedgehog chromosomes were only observed in the terminal position of all the chromosomes in both species. Conclusion Our observations provide new information on the level of diversity within the Erinaceidae family.

scholarly journals Comparative Karyotype Analysis of Grasshoppers in the Genus Oxya (Orthoptera, Catantopidae) by Differential Staining Techniques

CYTOLOGIA ◽  
2005 ◽  
Vol 70 (1) ◽  
pp. 109-117 ◽  
Author(s):  
Aya Yoshimura ◽  
Yoshitaka Obara ◽  
Yoshikazu Ando ◽  
Hiroshi Kayano
Keyword(s):  
Karyotype Analysis ◽  
Differential Staining ◽  
Staining Techniques

A Study of the Ultrastructure of Visual Cell Outer Segment Membranes, Using a Water-Miscible Embedding Medium and Differential Staining

1972 ◽  
Vol 30 ◽  
pp. 50-51
Author(s):  
Anthony J. Godfrey
Keyword(s):  
Outer Segment ◽  
Protein Denaturation ◽  
Uranyl Acetate ◽  
Differential Staining ◽  
Visual Cell ◽  
Lead Citrate ◽  
Chick Retina ◽  
Dark Staining ◽  
Embedding Medium ◽  
The Individual

Aldehyde-fixed chick retina was embedded in a water-containing resin of glutaraldehyde and urea, without dehydration. The loss of lipids and other soluble tissue components, which is severe in routine methods involving dehydration, was thereby minimized. Osmium tetroxide post-fixation was not used, lessening the amount of protein denaturation which occurred. Ultrathin sections were stained with 1, uranyl acetate and lead citrate, 2, silicotungstic acid, or 3, osmium vapor, prior to electron microscope examination of visual cell outer segment ultrastructure, at magnifications up to 800,000.Sections stained with uranyl acetate and lead citrate (Fig. 1) showed that the individual disc membranes consisted of a central lipid core about 78Å thick in which dark-staining 40Å masses appeared to be embedded from either side.

Gloriosa superba-made anticancer molecules

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
G Sivakumar
Keyword(s):  
Gloriosa Superba

Cost and Returns from the Cultivation of Gloriosa Superba

2012 ◽  
Vol 2 (6) ◽  
pp. 127-128
Author(s):  
Dr.B.Vanitha Dr.B.Vanitha ◽  
◽  
P. Manimalathi P. Manimalathi
Keyword(s):  
Gloriosa Superba

Evaluation of Gloriosa superba for Yield Attributing Characters and Quantification of Colchicine Originated from Different Agro Climatic Zones of Tamil Nadu and Andhra Pradesh

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Arun Kumar P. ◽  
Elangaimannan R.
Keyword(s):  
Seed Yield ◽  
Tamil Nadu ◽  
High Performance ◽  
Andhra Pradesh ◽  
Crop Improvement ◽  
Mean Value ◽  
Hplc Method ◽  
Gloriosa Superba ◽  
Climatic Zones ◽  
Colchicine Alkaloid

The study was conducted to evolve Gloriosa superba for yield characters and alkalodi content for selecting elite genotypes for comercial exploitatio n. The genotypes were sowm in Variyankaval village, Udayarpalayam taluk of Ariyalur district, Tamil Nadu. The highest mean value for fresh and dry seed yield was observed in Chittor local. The genotype Mulanur local has recorded the highest mean value for number of pods per plant and number of seeds per pod and Arupukotai local excelled the general mean for the traits seeds per pod, fresh and dry seed yield and also for tuber characters. An investigation was carried out to quantify the colchicine (alkaloid) present in tubers by High Performance Liquid Chromatography (HPLC) method. The genotypes collected from Arupukotai recorded the highest colchicine content (0.760 mg/g) followed by Chittoor (0.578 mg/g) and Mulanur (0.496 mg/g) and there by these three genotypes were utilized for further crop improvement.

Detection Y Chromosome Microdeletions Among Iraq Population in Infertile Patients with Azoospermia and Severe Oligospermia

2019 ◽  
Vol 9 (02) ◽  
Author(s):  
Samah A Hammood ◽  
Alaauldeen S M AL-Sallami ◽  
Saleh M Al-Khafaji
Keyword(s):  
Y Chromosome ◽  
Karyotype Analysis ◽  
Semen Analysis ◽  
Obstructive Azoospermia ◽  
Severe Oligozoospermia ◽  
Infertile Men ◽  
Y Chromosome Microdeletions ◽  
Detailed Evaluation ◽  
Health Organization ◽  
Infertile Patients

Objective: To detection of microdeletions of Y chromosome and study the frequency of microdeletions in infertile men with non-obstructive azoospermia or severe oligozoospermia(Middle Euphrates center)in Iraq population. Material and methods: 153 males were included in the study, the casesweredivided into groups according to the infertility etiology and semen analysis according to Word health organization, the frequencies and the characteristicsof Y chromosome microdeletions were investigated in groups. Multiplex PCR was applied to detect the microdeletions. Results:Y chromosome microdeletion was detected in 42 (40.7%) of 153 cases ,Microdeletions in azoospermia showed more frequently detected 28 (52.8%), followed by severe oligospermia 14 (28 %),Microdeletions in the AZFc region were the most common 12 (22.64%), followed by AZFb 11(20.75%) and AZFa 5(9.43%) in azoospermia compared to severe oligospermisAZFc 6 (12%) AZFb 4 (8 %) and AZFa 4 (8%). Conclusion: Y chromosome microdeletions were detected quite frequently in certain infertility subgroups. Therefore, detailed evaluation of an infertile man by physical examination, semen analysis, hormonal evaluationsand when required, karyotype analysis may predict the patients for whom Y chromosome microdeletionanalysis is necessary and also prevent cost increases. Recommendation: This study emphasizes that analysis of microdeletions should be carried out for all patients with idiopathic azoospermia and severe oligospermia who are candidates for intracytoplasmic sperm injection

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