Coupling scanning electron microscopy with DNA bar coding: a novel approach for thrips identification

2014 ◽  
Vol 49 (3) ◽  
pp. 403-409 ◽  
Author(s):  
Vivek Kumar ◽  
Dakshina R. Seal ◽  
Lance S. Osborne ◽  
Cindy L. McKenzie
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Novel Approach ◽  
Dna Bar Coding ◽  
Scanning Electron

scholarly journals Detection of Phenols from Industrial Effluents Using Streptomyces Mediated Gold Nanoparticles

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Bi Bi Zainab Mazhari ◽  
Dayanand Agsar
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Gold Nanoparticles ◽  
Visual Detection ◽  
Industrial Effluents ◽  
Sodium Sulphate ◽  
X Ray ◽  
Novel Approach ◽  
Average Size ◽  
Scanning Electron

Extracellular gold nanoparticles synthesized by Streptomyces tuirus DBZ39 were explored for the detection of phenols in the effluent of fertilizer and distillery industries. An average size of 27–56 nm gold nanoparticles was produced and confirmed by UV-vis absorption spectrum, scanning electron microscopy, and energy dispersive X-ray analysis. In the present investigation visual detection of phenols in the effluent samples by gold nanoparticles is enhanced by sodium sulphate. The detection is achieved successfully within 2 min, with change in color of the effluent samples. Use of biologically originated gold nanoparticles along with salt for the detection of phenols from industrial effluents is a novel approach.

Structure and Plastic Strain Analysis Utilizing Scanning Electron Microscopy

1972 ◽  
Vol 30 ◽  
pp. 666-667
Author(s):  
Frederick E. Schmidt ◽  
Alan Lawlev
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Strain Analysis ◽  
Forming Limit ◽  
Forming Process ◽  
Localized State ◽  
Novel Approach ◽  
Master Pattern ◽  
Scanning Electron

‘Forming limit’ or ‘instability limit’ diagrams are particularly useful in the processing of sheet materials by deep drawing or stretch forming. The key to the development of these diagrams is a quantitative characterization of the localized state of strain at various stages throughout the forming process. A novel approach has been developed whereby the distortions of a high-resolution grid, i.e. the strain analysis, are accurately monitored by scanning electron microscopy. A concurrent characterization of the associated surface structure is obtained.The grid for strain analysis is produced by a photoresist technique. Any practical grid master pattern may be used; the most useful shapes for the current studies are rectangles, squares, or circles with a resolution to 200 lines per inch. Photoresist emulsion is applied directly to the sheet specimen surface and air cured. The master pattern is then held secure against the emulsion by vacuum and the composite assembly exposed to ultraviolet light.

A novel approach to scanning electron microscopy at ambient atmospheric pressure

Microscopy ◽  
2014 ◽  
Vol 64 (2) ◽  
pp. 97-104 ◽  
Author(s):  
Yusuke Ominami ◽  
Shinsuke Kawanishi ◽  
Tatsuo Ushiki ◽  
Sukehiro Ito
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Atmospheric Pressure ◽  
Novel Approach ◽  
Scanning Electron

scholarly journals A novel approach to the histological diagnosis of pediatric nephrotic syndrome by low vacuum scanning electron microscopy

2014 ◽  
Vol 35 (4) ◽  
pp. 227-236 ◽  
Author(s):  
Shinichi OKADA ◽  
Sumire INAGA ◽  
Yasuo KAWABA ◽  
Takuya HANADA ◽  
Atsushi HAYASHI ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nephrotic Syndrome ◽  
Histological Diagnosis ◽  
Novel Approach ◽  
Scanning Electron

scholarly journals A novel approach for scanning electron microscopy of colloidal gold-labeled cell surfaces.

1984 ◽  
Vol 99 (1) ◽  
pp. 53-57 ◽  
Author(s):  
E de Harven ◽  
R Leung ◽  
H Christensen
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Surface ◽  
Colloidal Gold ◽  
Protein A ◽  
Staphylococcal Protein A ◽  
Good Resolution ◽  
Novel Approach ◽  
Backscattered Electron ◽  
Scanning Electron

A method is described for the use of scanning electron microscopy on the surface of gold-labeled cells. It includes the use of 45- or 20-nm colloidal gold marker conjugated with Staphylococcal protein A. The marker is best recognized on the basis of its atomic number contrast by using the backscattered electron imaging mode of the scanning electron microscope. When the backscattered electron signal is mixed with the secondary electron signal, an optimum correlation between the distribution of the labeled sites and the cell surface structures is demonstrated. The method is illustrated by its application to the identification of human circulating granulocytes. Its good resolution, high contrast, and good labeling efficiency offers a promising approach to the specific localization of cell surface antigenic sites labeled with particles of colloidal gold.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

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