Sample Preparation of Corn Seed Tissue to Prevent Analyte Relocations for Mass Spectrometry Imaging

2017 ◽  
Vol 28 (8) ◽  
pp. 1729-1732 ◽  
Author(s):  
Shin Hye Kim ◽  
Jeongkwon Kim ◽  
Young Jin Lee ◽  
Tae Geol Lee ◽  
Sohee Yoon
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Mass Spectrometry Imaging ◽  
Corn Seed

scholarly journals Sample preparation strategy for the detection of steroid-like compounds using MALDI mass spectrometry imaging: pulmonary distribution of budesonide as a case study

2021 ◽  
Author(s):  
Riccardo Zecchi ◽  
Pietro Franceschi ◽  
Laura Tigli ◽  
Davide Amidani ◽  
Chiara Catozzi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Mass Spectrometry Imaging ◽  
Maldi Mass Spectrometry ◽  
Adult Rabbit ◽  
Chronic Obstructive ◽  
Ionization Mass ◽  
Obstructive Pulmonary Disease ◽  
Imaging Protocol ◽  
Girard’S Reagent

AbstractCorticosteroids as budesonide can be effective in reducing topic inflammation processes in different organs. Therapeutic use of budesonide in respiratory diseases, like asthma, chronic obstructive pulmonary disease, and allergic rhinitis is well known. However, the pulmonary distribution of budesonide is not well understood, mainly due to the difficulties in tracing the molecule in lung samples without the addition of a label. In this paper, we present a matrix-assisted laser desorption/ionization mass spectrometry imaging protocol that can be used to visualize the pulmonary distribution of budesonide administered to a surfactant-depleted adult rabbit. Considering that budesonide is not easily ionized by MALDI, we developed an on-tissue derivatization method with Girard’s reagent P followed by ferulic acid deposition as MALDI matrix. Interestingly, this sample preparation protocol results as a very effective strategy to raise the sensitivity towards not only budesonide but also other corticosteroids, allowing us to track its distribution and quantify the drug inside lung samples. Graphical abstract

Characterization of firearm discharge residues recovered from skin swabs using sub-micrometric mass spectrometry imaging

2016 ◽  
Vol 8 (21) ◽  
pp. 4300-4305 ◽  
Author(s):  
Anthony Castellanos ◽  
Suzanne Bell ◽  
Francisco Fernandez-Lima
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Mass Spectrometry Imaging ◽  
Tof Sims

MSI-TOF-SIMS permits the analysis of skin swabs containing GSR (or not) and OGSR without the need of sample preparation and with little to no damage to the surface of the skin swab (thus preserving the evidence for further analysis).

Development of a Knudsen-type matrix coater for sample preparation for mass spectrometry imaging

2018 ◽  
Vol 13 (3) ◽  
pp. 03B407
Author(s):  
Martin Körsgen ◽  
Bonnie J. Tyler ◽  
Marcel Heeger ◽  
Moritz Buhl ◽  
Heinrich F. Arlinghaus
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Mass Spectrometry Imaging

scholarly journals Mapping the Chemistry of Hair Strands by Mass Spectrometry Imaging—A Review

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7522
Author(s):  
Mai H. Philipsen ◽  
Emma R. Haxen ◽  
Auraya Manaprasertsak ◽  
Per Malmberg ◽  
Emma U. Hammarlund
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Mass Spectrometry Imaging ◽  
Spatial Information ◽  
High Spatial Resolution ◽  
Matrix Effects ◽  
Forensic Sciences ◽  
Chemical Information ◽  
Hair Samples ◽  
Hair Strand

Hair can record chemical information reflecting our living conditions, and, therefore, strands of hair have become a potent analytical target within the biological and forensic sciences. While early efforts focused on analyzing complete hair strands in bulk, high spatial resolution mass spectrometry imaging (MSI) has recently come to the forefront of chemical hair-strand analysis. MSI techniques offer a localized analysis, requiring fewer de-contamination procedures per default and making it possible to map the distribution of analytes on and within individual hair strands. Applying the techniques to hair samples has proven particularly useful in investigations quantifying the exposure to, and uptake of, toxins or drugs. Overall, MSI, combined with optimized sample preparation protocols, has improved precision and accuracy for identifying several elemental and molecular species in single strands of hair. Here, we review different sample preparation protocols and use cases with a view to make the methodology more accessible to researchers outside of the field of forensic science. We conclude that—although some challenges remain, including contamination issues and matrix effects—MSI offers unique opportunities for obtaining highly resolved spatial information of several compounds simultaneously across hair surfaces.

scholarly journals Neuropeptidomics: Improvements in Mass Spectrometry Imaging Analysis and Recent Advancements

2020 ◽  
Vol 21 ◽  
Author(s):  
Nhu Q. Vu ◽  
Kellen DeLaney ◽  
Lingjun Li
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Functional Role ◽  
Mass Spectrometry Imaging ◽  
Analytical Techniques ◽  
Model Organisms ◽  
Preparation Technique ◽  
Single Experiment ◽  
Recent Developments ◽  
Msi Analysis

: Neuropeptides are an important class of endogenous peptides in the nervous system that regulate physiological functions such as feeding, glucose homeostasis, pain, memory, reproduction, and many others. In order to understand the functional role of neuropeptides in diseases or disorders, studies investigating their dysregulation in terms of changes in abundance and localization must be carried out. Because multiple neuropeptides are believed to play a functional role in each physiological process, techniques capable of globally profiling multiple neuropeptides simultaneously are desired. Mass spectrometry is well-suited for this goal due to its ability to perform untargeted measurements without prior comprehensive knowledge of the analytes of interest. Mass spectrometry imaging (MSI) is particularly useful because it has the capability to image a large variety of peptides in a single experiment without labeling. Like all analytical techniques, careful sample preparation is critical to successful MSI analysis. The first half of this review focuses on recent developments in MSI sample preparation and instrumentation for analyzing neuropeptides and other biomolecules in which the sample preparation technique may be directly applicable for neuropeptide analysis. The benefit offered by incorporating these techniques is shown as improvement in number of observable neuropeptides, enhanced signal to noise, increased spatial resolution, or a combination of these aspects. The second half of this review focuses on recent biological discoveries about neuropeptides resulting from these improvements in MSI analysis. The recent progress in neuropeptide detection and analysis methods, including incorporation of various tissue washes, matrices, instruments, ionization sources, and computation approaches combined with the advancements in understanding neuropeptide function in a variety of model organisms indicates potential for the utilization of MSI analysis of neuropeptides in clinical settings.

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