Pyramiding Ty-1/Ty-3, Ty-2, ty-5 and ty-6 genes into tomato hybrid to develop resistance against tomato leaf curl viruses and recurrent parent genome recovery by ddRAD sequencing method

Author(s):  
Pardhasaradhi Prabhandakavi ◽  
Rambabu Pogiri ◽  
Rakesh Kumar ◽  
Santanu Acharya ◽  
Ramchandran Esakky ◽  
...  
Author(s):  
Ravinder Kumar ◽  
Rahul Kumar Tiwari ◽  
Arjunan Jeevalatha ◽  
Sundaresha Siddappa ◽  
Mohd. Abas Shah ◽  
...  

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Edith Khamonya Avedi ◽  
Adedapo Olutola Adediji ◽  
Dora Chao Kilalo ◽  
Florence Mmogi Olubayo ◽  
Isaac Macharia ◽  
...  

Abstract Background Tomato production is threatened worldwide by the occurrence of begomoviruses which are associated with tomato leaf curl diseases. There is little information on the molecular properties of tomato begomoviruses in Kenya, hence we investigated the population and genetic diversity of begomoviruses associated with tomato leaf curl in Kenya. Methods Tomato leaf samples with virus-like symptoms were obtained from farmers’ field across the country in 2018 and Illumina sequencing undertaken to determine the genetic diversity of associated begomoviruses. Additionally, the occurrence of selection pressure and recombinant isolates within the population were also evaluated. Results Twelve complete begomovirus genomes were obtained from our samples with an average coverage of 99.9%. The sequences showed 95.7–99.7% identity among each other and 95.9–98.9% similarities with a Tomato leaf curl virus Arusha virus (ToLCArV) isolate from Tanzania. Analysis of amino acid sequences showed the highest identities in the regions coding for the coat protein gene (98.5–100%) within the isolates, and 97.1–100% identity with the C4 gene of ToLCArV. Phylogenetic algorithms clustered all Kenyan isolates in the same clades with ToLCArV, thus confirming the isolates to be a variant of the virus. There was no evidence of recombination within our isolates. Estimation of selection pressure within the virus population revealed the occurrence of negative or purifying selection in five out of the six coding regions of the sequences. Conclusions The begomovirus associated with tomato leaf curl diseases of tomato in Kenya is a variant of ToLCArV, possibly originating from Tanzania. There is low genetic diversity within the virus population and this information is useful in the development of appropriate management strategies for the disease in the country.


Author(s):  
Yamuna Hanamasagar ◽  
Priya Naganur ◽  
K. S. Shankarappa ◽  
V. Venkataravanappa ◽  
C. N. Lakshminarayana Reddy

2009 ◽  
Vol 53 (2) ◽  
pp. 99-104 ◽  
Author(s):  
H. Tamarzizt ◽  
S. Chouchane ◽  
R. Lengliz ◽  
D. Maxwell ◽  
M. Marrakchi ◽  
...  

Author(s):  

Abstract A new distribution map is provided for Tomato leaf curl New Delhi virus. Geminiviridae: Begomovirus. Hosts: tomato (Solanum lycopersicum) and other Solanaceae such as aubergine (S. melongena), potato (S. tuberosum), Capsicum spp. and Cucurbitaceae. Information is given on the geographical distribution in Europe (Italy, Sicily, Spain, Mainland Spain), Asia (Bangladesh, India, Andhra Pradesh, Delhi, Gujarat, Haryana, Karnataka, Maharashtra, Punjab, Tamil Nadu, Uttar Pradesh, West Bengal, Indonesia, Java, Iran, Pakistan, Philippines, Sri Lanka, Taiwan and Thailand) and Africa (Tunisia).


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