Using cytogenetic analysis to identify the genetic diversity in Lilium hansonii (Liliaceae), an endemic species of Ulleung Island, Korea

2021 ◽  
Author(s):  
Yu-Hyeon Choi ◽  
Fahad Ramzan ◽  
Yoon-Jung Hwang ◽  
Adnan Younis ◽  
Chang-Kil Kim ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Endemic Species ◽  
Cytogenetic Analysis ◽  
Ulleung Island

scholarly journals Primer Binding Site (PBS) Profiling of Genetic Diversity of Natural Populations of Endemic Species Allium ledebourianum Schult.

BioTech ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 23
Author(s):  
Oxana Khapilina ◽  
Ainur Turzhanova ◽  
Alevtina Danilova ◽  
Asem Tumenbayeva ◽  
Vladislav Shevtsov ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Endemic Species ◽  
Biological Diversity ◽  
Natural Populations ◽  
Subsequent Development ◽  
Dna Profiling ◽  
Primer Binding Site ◽  
Anthropogenic Factors ◽  
Habitat Specificity ◽  
Interspersed Repeats

Endemic species are especially vulnerable to biodiversity loss caused by isolation or habitat specificity, small population size, and anthropogenic factors. Endemic species biodiversity analysis has a critically important global value for the development of conservation strategies. The rare onion Allium ledebourianum is a narrow-lined endemic species, with natural populations located in the extreme climatic conditions of the Kazakh Altai. A. ledebourianum populations are decreasing everywhere due to anthropogenic impact, and therefore, this species requires preservation and protection. Conservation of this rare species is associated with monitoring studies to investigate the genetic diversity of natural populations. Fundamental components of eukaryote genome include multiple classes of interspersed repeats. Various PCR-based DNA fingerprinting methods are used to detect chromosomal changes related to recombination processes of these interspersed elements. These methods are based on interspersed repeat sequences and are an effective approach for assessing the biological diversity of plants and their variability. We applied DNA profiling approaches based on conservative sequences of interspersed repeats to assess the genetic diversity of natural A. ledebourianum populations located in the territory of Kazakhstan Altai. The analysis of natural A. ledebourianum populations, carried out using the DNA profiling approach, allowed the effective differentiation of the populations and assessment of their genetic diversity. We used conservative sequences of tRNA primer binding sites (PBS) of the long-terminal repeat (LTR) retrotransposons as PCR primers. Amplification using the three most effective PBS primers generated 628 PCR amplicons, with an average of 209 amplicons. The average polymorphism level varied from 34% to 40% for all studied samples. Resolution analysis of the PBS primers showed all of them to have high or medium polymorphism levels, which varied from 0.763 to 0.965. Results of the molecular analysis of variance showed that the general biodiversity of A. ledebourianum populations is due to interpopulation (67%) and intrapopulation (33%) differences. The revealed genetic diversity was higher in the most distant population of A. ledebourianum LD64, located on the Sarymsakty ridge of Southern Altai. This is the first genetic diversity study of the endemic species A. ledebourianum using DNA profiling approaches. This work allowed us to collect new genetic data on the structure of A. ledebourianum populations in the Altai for subsequent development of preservation strategies to enhance the reproduction of this relict species. The results will be useful for the conservation and exploitation of this species, serving as the basis for further studies of its evolution and ecology.

scholarly journals Comparative Population Genetics of Two Korean Aedes Mosquito Species with Vector Potential based on Mitochondrial DNA

2021 ◽  
Author(s):  
Jiyeong Shin ◽  
Jongwoo Jung
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Vector Control ◽  
Endemic Species ◽  
Korean Peninsula ◽  
Mismatch Distribution ◽  
Mosquito Species ◽  
Mitochondrial Genes ◽  
Rapid Expansion ◽  
Number Of Individuals

Abstract Background: Mosquitoes of the genus Aedes are important invasive species contributing to the spread of chikungunya, dengue fever, yellow fever, Zika virus, and other dangerous vector-borne diseases. Aedes albopictus is native to southeast Asia with rapid expansion due to human activity, showing a wide distribution in the Korean peninsula. Aedes flavopictus is considered to be native to East Asia with a broad distribution in the region, including in the Korean peninsula. Gaining a better understanding of the genetic diversity of these species is critical for establishing strategies for disease prevention and vector control. Methods: We obtained DNA from 148 specimens of Ae. albopictus and 166 specimens of Ae. flavopictus in Korea, and amplified two mitochondrial genes (COI and ND5) to compare the genetic diversity and structure of the two species.Results: We obtained a 658-bp sequence of COI and a 423-bp sequence of ND5 from the two mosquito species. We found low diversity and an insignificant population genetic structure in Ae. albopictus, and high diversity and an insignificant structure in Ae. flavopictus for these two mitochondrial genes. Ae. albopictus had less haplotypes with respect to the number of individuals, and a slight mismatch distribution was confirmed. By contrast, Ae. flavopictus had a large number of haplotypes compared with the number of individuals, and a large unimodal-type mismatch distribution was confirmed. Although the genetic structure of both species was insignificant, Ae. flavopictus exhibited higher genetic diversity than Ae. albopictus.Conclusions: Ae. albopictus appears to be an introduced species, whereas Ae. flavopictus is an endemic species to the Korean peninsula, and the difference in genetic diversity between the two species is related to their adaptability and introduction history. As an endemic species, Ae. flavopictus is likely to have a larger population size than expected. Further studies on the genetic structure and diversity of these two mosquito species will provide useful data for vector control.

RAPD-based evaluation of genetic diversity among populations of the Iranian endemic species Rhabdosciadium aucheri Boiss. (Apiaceae)

2020 ◽  
Vol 47 (12) ◽  
pp. 9345-9352
Author(s):  
Fatemeh Kazemeini ◽  
Younes Asri ◽  
Golaleh Mostafavi ◽  
Ramezan Kalvandi ◽  
Iraj Mehregan
Keyword(s):  
Genetic Diversity ◽  
Endemic Species

Genetic diversity and structure of Hypochaeris catharinensis Cabrera (Asteraceae), an endemic species of Southern Brazil

2019 ◽  
Vol 305 (8) ◽  
pp. 675-686
Author(s):  
Camila L. Chaves ◽  
Eduardo A. Ruas ◽  
Claudete F. Ruas ◽  
Estrella Urtubey ◽  
Paulo M. Ruas
Keyword(s):  
Genetic Diversity ◽  
Endemic Species ◽  
Southern Brazil ◽  
Genetic Diversity And Structure

scholarly journals Selection of ISSR Primers to Study the Genetic Diversity of Myrciaria floribunda O. Berg

2020 ◽  
pp. 23-28
Author(s):  
Luiz Sergio Costa Duarte Filho ◽  
Danielson Ramos Ribeiro ◽  
Allison Vieira da Silva
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
South America ◽  
Central America ◽  
Dna Extraction ◽  
Endemic Species ◽  
Germplasm Bank ◽  
Slow Growing ◽  
Selection Of ◽  
Issr Primers

The species Myrciaria floridunda O. Berg, popularly known as cambuí, belongs to the Myrtaceae family. Cambui is a native, non-endemic species that occur in diverse environments in Central America and South America. They are slow-growing plants with a shrub or sub-shrub habit. The fruits, the product of interest of the species, are small, spherical berries orange or red in colorturning to wine colorwhen they are ripe. The exploitation of the species is still mostly extractivist, carried out by traditional local families who, in times of fruiting of the species, leverage their income by selling fruits at fairs. The fruits can be eaten fresh, in the form of jellies, liquor or wine. To study the genetic diversity of the species using ISSR-type molecular markers, it is necessary to first isolate DNA in sufficient quality and quantity. Here, leaves for DNA extraction were collected from the active germplasm bank of the Federal University of Alagoas, Brazil. The DNA of the species was extracted using CTAB detergent methodology with modifications adapted to the species. Twelve ISSR primers were tested on DNA from two cambui genotypes. Of the twelve primers, eight were selected due to their polymorphism index above 50%, namely: UFAL-2, UFAL-3, UFAL-5, UFAL-6, UFAL-7, UFAL-8, UFAL-9 and UFAL-10.

scholarly journals Genetic Diversity and Structure of the Korean Rare and Endemic Species, Deutzia pdaniculata Nakai, as Revealed by ISSR Markers

2013 ◽  
Vol 26 (5) ◽  
pp. 619-627
Author(s):  
Sung-Won Son ◽  
Kyoung Su Choi ◽  
Kyu Tae Park ◽  
Eun-Hye Kim ◽  
Seon Joo Park
Keyword(s):  
Genetic Diversity ◽  
Endemic Species ◽  
Issr Markers ◽  
Genetic Diversity And Structure

scholarly journals Genetic Diversity of Endophytic Fungal Strains Isolated from the Roots of Coastal Plants in Ulleung Island for Restoration of Coastal Ecosystem

2012 ◽  
Vol 22 (10) ◽  
pp. 1384-1391 ◽  
Author(s):  
Miae Kim ◽  
Young-Hyun You ◽  
Hyeokjun Yoon ◽  
Hyun Kim ◽  
Yeonggyo Seo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Coastal Ecosystem ◽  
Coastal Plants ◽  
Fungal Strains ◽  
Ulleung Island

scholarly journals Comparative genetic and epigenetic diversity in pairs of sympatric, closely related plants with contrasting distribution ranges in south-eastern Iberian mountains

AoB Plants ◽  
2020 ◽  
Vol 12 (3) ◽  
Author(s):  
Mónica Medrano ◽  
Conchita Alonso ◽  
Pilar Bazaga ◽  
Esmeralda López ◽  
Carlos M Herrera
Keyword(s):  
Genetic Diversity ◽  
Genetic Polymorphism ◽  
Endemic Species ◽  
Phenotypic Diversity ◽  
Wild Plants ◽  
Epigenetic Variation ◽  
Widespread Species ◽  
South Eastern ◽  
Distribution Ranges ◽  
Epigenetic Diversity

Abstract Genetic diversity defines the evolutionary potential of a species, yet mounting evidence suggests that epigenetic diversity could also contribute to adaptation. Elucidating the complex interplay between genetic and epigenetic variation in wild populations remains a challenge for evolutionary biologists, and the intriguing possibility that epigenetic diversity could compensate for the loss of genetic diversity is one aspect that remains basically unexplored in wild plants. This hypothesis is addressed in this paper by comparing the extent and patterns of genetic and epigenetic diversity of phylogenetically closely related but ecologically disparate species. Seven pairs of congeneric species from Cazorla mountains in south-eastern Spain were studied, each pair consisting of one endemic, restricted-range species associated to stressful environments, and one widespread species occupying more favourable habitats. The prediction was tested that endemic species should have lower genetic diversity due to population fragmentation, and higher epigenetic diversity induced by environmental stress, than their widespread congeners. Genetic (DNA sequence variants) and epigenetic (DNA cytosine methylation variants) diversities and their possible co-variation were assessed in three populations of each focal species using amplified fragment length polymorphism (AFLP) and methylation-sensitive AFLP (MSAP). All species and populations exhibited moderate to high levels of genetic polymorphism irrespective of their ecological characteristics. Epigenetic diversity was greater than genetic diversity in all cases. Only in endemic species were the two variables positively related, but the difference between epigenetic and genetic diversity was greater at populations with low genetic polymorphism. Results revealed that the relationship between genetic and epigenetic diversity can be more complex than envisaged by the simple hypothesis addressed in this study, and highlight the need of additional research on the actual role of epigenetic variation as a source of phenotypic diversity before a realistic understanding of the evolutionary relevance of epigenetic phenomena in plant adaptation can be achieved.

Assessment of genetic diversity, population structure and morphological analyses in an Iranian endemic species Rhabdosciadium aucheri Boiss. (Apiaceae) using ISSR markers

Biologia ◽  
2020 ◽  
Vol 76 (2) ◽  
pp. 441-451
Author(s):  
Fatemeh Kazemeini ◽  
Younes Asri ◽  
Golaleh Mostafavi ◽  
Ramezan Kalvandi ◽  
Iraj Mehregan
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Endemic Species ◽  
Issr Markers
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