Sensory optimization of formulated coffee mix from pedada seed (Sonneratia caseolaris) and robusta coffee (Coffea canephora)

Pedada seed is one of potential anti-oxidant resources of coastal plants which is under-utilized. In this current study, pedada seed was formulated in to Robusta coffee to develop a novel coffee mix. The purpose of this study was to obtain the most ideal formula of pedada-Robusta coffee mix based on sensory evaluation using Just about Right (JAR) analysis of French-press brewed coffee mix. The best coffee mix formula was obtained for the mix of 1:1 proportion between pedada seed and Robusta coffee. There were 18 out of 19 sensory attributes of the best formula that sensorially accepted by the consumer respondents. Those included vegetative aroma, brown sugar aroma, musty aroma, sweet aroma, nutty aroma, cocoa aroma, citrus-fruit aroma, sweet taste, sour taste, body-mouth-feel, mouth-feel strength, nutty flavour, burnt flavour, chemical flavour, citrus-fruit flavour, beany flavour, roasted flavour and sweet flavour. The best pedada-coffee mix was also characterized as having mean particle size of 421.5±19.1 μm, total phenolic content of 21.55±0.21 mg GAE/g, antioxidant activity of 48.60±0.94%, caffeine content of 278.50 ± 4.95 mg/kg, total dissolved solids of 0.4±0.066% and colour with a value of L=17.0±0.0; a=18.6±0.9 and b=23.6±0.9.

Overcoming limitations to environmental DNA studies: A coastal temperate reference sequence database for multiple chloroplast gene regions generated in a single assay.

A proliferation in environmental DNA (eDNA) research has increased the reliance on reference sequence databases to assign unknown DNA sequences to known taxa. Without comprehensive reference databases, DNA extracted from environmental samples cannot be correctly assigned to taxa, limiting the use of this genetic information to identify organisms in unknown sample mixtures. For animals, standard metabarcoding practices involve amplification of the mitochondrial Cytochrome-c oxidase subunit 1 (CO1) region, which is a universally amplifyable region across majority of animal taxa. This region, however, does not work well as a DNA barcode for plants and fungi, and there is no similar universal single barcode locus that has the same species resolution. Therefore, generating reference sequences has been more difficult and several loci have been suggested to be used in parallel to get to species identification. For this reason, we developed a multi-gene targeted capture approach to generate reference DNA sequences for plant taxa across 20 target chloroplast gene regions in a single assay. We successfully compiled a reference database for 93 temperate coastal plants including seagrasses, mangroves, and saltmarshes/samphire’s. We demonstrate the importance of a comprehensive reference database to prevent species going undetected in eDNA studies. We also investigate how using multiple chloroplast gene regions impacts the ability to discriminate between taxa.

Population subdivision promoted by a sea-level-change-driven bottleneck: a glimpse from the evolutionary history of the mangrove tree Aegiceras corniculatum

Historic climate changes had always driven geographical populations of coastal plants to contract and recover dynamically, even die out completely. Species suffering from such bottlenecks usually lose intraspecific genetic diversity, but how do these events influence population subdivision patterns of coastal plants? We investigated this question in the typical coastal plant: mangrove species Aegiceras corniculatum. Inhabiting the intertidal zone of the tropical and subtropical coast of the Indo-West Pacific oceans, its populations are deemed to be greatly shaped by historic sea-level fluctuations. Using dual methods of Sanger and Illumina Solexa sequencing, we found that the 18 sampled populations were structured into two groups, namely, the “Indo-Malayan” group, comprising three subgroups (the northern South China Sea, Gulf of Bengal, and Bali), and the “Pan-Australasia” group, comprising the subgroups of the southern South China Sea and Australasia. Based on simulations using the approximate Bayesian computation method, we inferred that the southern South China Sea subgroup, which penetrates the interior of the “Indo-Malayan” group, originated from the Australasia subgroup, accompanied by a severe bottleneck event, with a spot of gene flow from both the Australasia and “Indo-Malayan” groups. Geographical barriers such as the Sundaland underlie the genetic break between Indian and Pacific Oceans, but the discontinuity between southern and northern South China Sea was originated from genetic drift in the bottleneck event. Hence, we revealed a case evidencing that the bottleneck event promoted population subdivision. This conclusion may be applicable in other taxa beyond coastal plants.

BAKTERI PATOGEN PADA KEPITING KELAPA (Birgus latro)

Coconut crab (Birgus latro) is a fishery resource with high economic value. Coconut Crab is only found in eastern Indonesia, covering Sulawesi, Maluku and North Maluku. One of the habitat of Coconut Crab in North Maluku is Moor Island, Central Halmahera. The coastal typology of this island is predominantly rocky with many gaps and small caves, as well as several steep slopes with dominant vegetation conditions of coconut, beach pandanus and other coastal plants. Walnut Crab is often used as a consumption ingredient because it has delicious taste and high nutritions. However, the use of crustaceans for consumption can also be dangerous. This is because crustaceans also contain several pathogenic bacteria. This study aimed to identify pathogenic bacteria isolated from Coconut Crab which is the largest crustacean in the world. 5 samples of Coconut Crab were taken from Moor Island. Isolated samples marked KK1, KK2, KK3, KK4 and KK5. Pure bacterial isolates were obtained from Coconut Crab samples, evaluated for colony type and identified based on biochemical characterization. The types of pathogenic bacteria identified in samples KK1, KK2, KK3, KK4 and KK5 were Escherichia coli, Pseudomonas sp. and Staphylococcus sp.

Antibacterial Potential of Bidara Laut (Ximenia americana) Plant Against Vibrio alginolyticus and V. parahaemolyticus Bacteria

Background: Vibriosis is currently still a major problem in marine aquaculture and is highly dependent on availability of antibacterial agents in its handling. Investigation of antibacterial potential of the coastal plants of Bidara Laut (Ximenia americana) has been carried out. Methods: Parts of the X. americana plant taken are Old Fruit, Young Fruit, Old Leaf, and Young Leaf. A total of ± 200 grams of each part of X. americana plant was taken to be extracted using maceration method. The antibacterial activity test was performed using the Kirby-Bauer disc diffusion method on Muller Hinton Agar (MHA) media. Observations were made in time units, namely every 6 hours of incubation for 24 hours. Results: The tested extract of plant parts of X. americana showed an inhibition zone against the growth of V. alginolyticus and V. parahaemolyticus bacteria in each replication in all-time units of observation. Of the four types of extracts tested, old leaves showed strong category resistance to V. alginolyticus with the highest clear zone diameter of 16 mm (average 14.67 mm) at 6 hours incubation. Young leaves gave the highest of 18 mm (average 16.33 mm) against V. parahaemolyticus at 6 hours incubation. Conclusions: X. americana plants have potential for antibacterial against Vibriosis disease in aquaculture. The ability of power to be seen tended to decrease for all tests until the end of observation, but until the end of observation, the ability of extract inhibition of all parts of the plant was still in the moderate to strong category.

ETHNOMEDICINE OF BAJO TRIBE COMMUNITY IN BANGKO VILLAGE, MAGINTI DISTRICT, WEST MUNA, SOUTHEAST SULAWESI

This study aims to reveal the use of marine and coastal plants, and animals as medicine by Bajo Tribe society in Bangko Village, West Muna. The method used was an exploratory survey. Data were collected using in-depth interviews with 20 traditional healers selected by purposive sampling. The data obtained were analyzed descriptively. The result revealed that there were 39 species of plants and 5 species of animals used as medicine. The most widely used plant families were the Arecaceae and Poaceae, 3 species respectively, while animals from the families of Holothuriidae, Onuphidae, Pinnidae, Syngnathidae, and Tridacnidae, 1 species respectively. The most commonly part of plant used was leaves (50%), while for animal was parts of flesh and whole body were used 40% respectively. The most commonly used preparation and application were boiling and drinking. In conclusion, Bajo people in Bangko Village with their maritime culture use plants and animals in the marine and coastal environment to overcome various diseases and health problems, in addition using “baca-baca” method.

Flora and Its Analysis. Hydrophilic Plants of Different Water Bodies of the Samarkand Region (Uzbekistan)

Aquatic and coastal aquatic plants are one of the most important components of aquatic ecosystems. Therefore, at present, much attention is paid to the issues of rational use and development of the country's inland water bodies. Together with phytoplankton, this group of plants participates in the trophic cycle of biocenosis, providing various stages of the links of the food chain with appropriate products. For example, the plants of reservoirs serve as food for representatives of different taxonomic groups of animals, mollusks, crustaceans, insects, fish, birds and mammals. With moderate overgrowing of water bodies, favorable conditions are formed for the development of the phytophilous fauna of invertebrates. The flora of higher aquatic and coastal plants of the Samarkand region has not yet been fully studied. The species composition was not studied, taxonomic and floristic analysis was not carried out. In addition, it is very rare to find detailed information about the use of aquatic plants in the national economy, information about useful species and rare species common in this territory. The article presents the results of research on the study of aquatic and coastal plants of reservoirs of the Samarkand region. It was revealed that 72 species of aquatic and coastal plants, which belong to 51 genera, 34 families and 5 classes, grow in reservoirs of various types on the territory of the Samarkand region. Representatives of the families Potamogetonaceae, Poaceae, Cyperaceae and Polygonaceae make up 43% of the total number of plants of this group growing in the studied region. In terms of life forms, about 12.5% (9 species) are annuals, and 77.8% (56 species) are perennials. It was found that 7 species of aquatic mosses grow in the studied water bodies.