Inhibitory effect of effective fraction of Salvia officinalis on aldose reductase activity: strategy to reduce complications of type 2 diabetes

2018 ◽  
Vol 19 (2) ◽  
pp. 211-216
Author(s):  
Somayeh Amraee ◽  
Seifollah Bahramikia
Keyword(s):  
Type 2 Diabetes ◽  
Aldose Reductase ◽  
Reductase Activity ◽  
Inhibitory Effect ◽  
Salvia Officinalis ◽  
Effective Fraction ◽  
Activity Strategy

scholarly journals Aldose Reductase Inhibitiory Effect of Gymnemic Acid, Trigonelline and Ferulic Acid- An In Silico Approach

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Roshana Devi Vellai ◽  
Subramanian Sorimuthu Pillai
Keyword(s):  
Type 2 Diabetes ◽  
Diabetic Retinopathy ◽  
Ferulic Acid ◽  
Aldose Reductase ◽  
In Silico ◽  
Inhibitory Effect ◽  
Computational Method ◽  
Gymnemic Acid ◽  
Chronic Hyperglycemia

Diabetic retinopathy (DR) is one of the earliest complications of chronic hyperglycemia and is a major cause of vision loss. Nearly all patients with type 1 diabetes mellitus and more than 60 % with chronic type 2 diabetes develop some degree of retinopathy after 10 years. Aldose reductase, the first enzyme in polyol pathway chiefly contributes to the development of diabetic retinopathy and other secondary complications. None of the currently available drugs used to inhibit the activity of aldose reductase is found to be ideal due to their adverse effects. Hence, the screening and identification of more effective and safer aldose reductase inhibitors from natural products have been critical requirement in the management and treatment of T2DM. Recently, we have reported the antidiabetic properties of phytochemicals such as Gymnemic acid, Trigonelline and Ferulic acid in high fat diet fed- low dose STZ induced experimental type 2 diabetes in rats. In the present study, the structure based computational method was employed to identify the in silico inhibitory effect of the above phytoingredients on aldose reductase activity. Auto Dock 4.2 is used to study the molecular interactions between the ligands and the receptor. The data obtained evidenced that the docking efficacy of the antidiabetic ligands which are comparable with fidarestat, the standard used in the present study. Thus, the antidiabetic properties of the above lead molecules may attribute to its aldose reductase inhibitory effect.

scholarly journals Astaxanthin inhibits aldose reductase activity in Psammomys obesus , a model of type 2 diabetes and diabetic retinopathy

2019 ◽  
Vol 7 (12) ◽  
pp. 3979-3985 ◽  
Author(s):  
Maha Benlarbi‐Ben Khedher ◽  
Khouloud Hajri ◽  
Ahmed Dellaa ◽  
Basma Baccouche ◽  
Imane Hammoum ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Diabetic Retinopathy ◽  
Aldose Reductase ◽  
Reductase Activity ◽  
Psammomys Obesus

scholarly journals Among Commercially Available Fruit Juices, Pomegranate Is the Most Effective Inhibitor of PMS-Trypsin Amyloid-Like Fibrils Formation

2019 ◽  
Vol 14 (6) ◽  
pp. 1934578X1985912
Author(s):  
Phanindra Babu Kasi ◽  
Márta Kotormán
Keyword(s):  
Type 2 Diabetes ◽  
Amyloid Fibrils ◽  
Binding Assay ◽  
Inhibitory Effect ◽  
Fruit Juices ◽  
Pomegranate Juice ◽  
Effective Inhibitor ◽  
Parkinson’S Diseases ◽  
Lateral Sclerosis

The formation of amyloid fibrils is associated with many human illnesses, such as Alzheimer’s, Huntington’s, and Parkinson’s diseases, amyotrophic lateral sclerosis, spongiform encephalitis, type 2 diabetes, and primary and secondary systemic amyloidosis. Nutrition contributes to the prevention of these diseases. The aim of our work was to look for commercially available fruit juices that can inhibit the formation of amyloid fibrils. Of the fruit juices that we examined, that of pomegranate was found to be the most effective inhibitory agent using turbidity measurements and Congo red binding assay. According to our experiments, pomegranate juice reduced the amount of PMS-trypsin amyloid-like fibrils to 3.7% at 5-fold dilution compared with the sample without pomegranate. The inhibitory effect of the pomegranate juice was concentration dependent.

scholarly journals Estimation of Aldose Reductase Activity and Malondialdehyde Levels in Patients with Type 2 Diabetes Mellitus

2019 ◽  
Vol 12 (2) ◽  
pp. 1001-1007
Author(s):  
Sandeep Kumar ◽  
Ajay Kumar ◽  
Mohammad Mustufa Khan
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Blood Sugar ◽  
Aldose Reductase ◽  
Pearson Correlation ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
P Value ◽  
Thiobarbituric Acid Reactive Substance

Type 2 diabetes mellitus (T2DM) is a metabolic disorder characterized by hyperglycemia due to insufficient secretion or action of insulin.Elevated oxidative stress and diminished antioxidants may play an important role to develop T2DM and its complications. Aldose reductase (AR) enzyme plays a key role in the reduction of glucose to sorbitol by Polyol pathway. To estimate the AR activity and malondialdehyde (MDA) levels and in patients with T2DM. In this case-control study, a total number of 60 subjects (30 T2DM and 30 age-matched controls) wererecruited.Fasting blood sugar (FBS), Post-Prandial blood sugar (PPBS), AR activity and MDA levels were estimated in all the subjects. The AR activity was estimated by nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidation method. The plasma MDA levels were estimated by the thiobarbituric acid reactive substance (TBARS) method. For Statistical analysis, all the data were compared between the two groups by using unpaired t-test. Pearson correlation coefficient was calculated among T2DM. A P value <0.05 was considered as statistically significant for all data analyzed. The mean of FBS, PPBS, AR activity, and MDA levels were found significantly high in T2DM as compared to controls (P<0.001, P<0.001, P<0.001, P<0.001, respectively). A significant positive correlation was found between FBS and PPBS among T2DM (r=0.71, P<0.01). However, There was no significant correlation found between AR activity and MDA level among T2DM (r=0.002, P>0.05). Results showed thatthe mean of FBS, PPBS, AR activity, and MDA levels were found significantly higher in T2DM than controls. There was no significant correlation found between AR activity and MDA level among T2DM.

scholarly journals Aldose Reductase Inhibitor, Epalrestat, Reduces Lipid Hydroperoxides in Type 2 Diabetes

2009 ◽  
Vol 56 (1) ◽  
pp. 149-156 ◽  
Author(s):  
Chie OHMURA ◽  
Hirotaka WATADA ◽  
Kosuke AZUMA ◽  
Tomoaki SHIMIZU ◽  
Akio KANAZAWA ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Aldose Reductase ◽  
Reductase Inhibitor ◽  
Aldose Reductase Inhibitor ◽  
Lipid Hydroperoxides

scholarly journals Investigation on the Enzymatic Profile of Mulberry Alkaloids by Enzymatic Study and Molecular Docking

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1776 ◽  
Author(s):  
Zhihua Liu ◽  
Ying Yang ◽  
Wujun Dong ◽  
Quan Liu ◽  
Renyun Wang ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Molecular Docking ◽  
Side Effects ◽  
Inhibitory Effect ◽  
Active Ingredients ◽  
Wide Range ◽  
Consensus Score

α-glucosidase inhibitors (AGIs) have been an important category of oral antidiabetic drugs being widely exploited for the effective management of type 2 diabetes mellitus. However, the marketed AGIs not only inhibited the disaccharidases, but also exhibited an excessive inhibitory effect on α-amylase, resulting in undesirable gastrointestinal side effects. Compared to these agents, Ramulus Mori alkaloids (SZ-A), was a group of effective alkaloids from natural Morus alba L., and showed excellent hypoglycemic effect and fewer side effects in the Phase II/III clinical trials. Thus, this paper aims to investigate the selective inhibitory effect and mechanism of SZ-A and its major active ingredients (1-DNJ, FA and DAB) on different α-glucosidases (α-amylase and disaccharidases) by using a combination of kinetic analysis and molecular docking approaches. From the results, SZ-A displayed a strong inhibitory effect on maltase and sucrase with an IC50 of 0.06 μg/mL and 0.03 μg/mL, respectively, which was similar to the positive control of acarbose with an IC50 of 0.07 μg/mL and 0.68 μg/mL. With regard to α-amylase, SZ-A exhibited no inhibitory activity at 100 μg/mL, while acarbose showed an obvious inhibitory effect with an IC50 of 1.74 μg/mL. The above analysis demonstrated that SZ-A could selectively inhibit disaccharidase to reduce hyperglycemia with a reversible competitive inhibition, which was primarily attributed to the three major active ingredients of SZ-A, especially 1-DNJ molecule. In the light of these findings, molecular docking study was utilized to analyze their inhibition mechanisms at molecular level. It pointed out that acarbose with a four-ring structure could perform desirable interactions with various α-glucosidases, while the three active ingredients of SZ-A, belonging to monocyclic compounds, had a high affinity to the active site of disaccharidases through forming a wide range of hydrogen bonds, whose affinity and consensus score with α-amylase was significantly lower than that of acarbose. Our study illustrates the selective inhibition mechanism of SZ-A on α-glucosidase for the first time, which is of great importance for the treatment of type 2 diabetes mellitus.

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