Estimation of Aldose Reductase Activity and Malondialdehyde Levels in Patients with Type 2 Diabetes Mellitus

Type 2 diabetes mellitus (T2DM) is a metabolic disorder characterized by hyperglycemia due to insufficient secretion or action of insulin.Elevated oxidative stress and diminished antioxidants may play an important role to develop T2DM and its complications. Aldose reductase (AR) enzyme plays a key role in the reduction of glucose to sorbitol by Polyol pathway. To estimate the AR activity and malondialdehyde (MDA) levels and in patients with T2DM. In this case-control study, a total number of 60 subjects (30 T2DM and 30 age-matched controls) wererecruited.Fasting blood sugar (FBS), Post-Prandial blood sugar (PPBS), AR activity and MDA levels were estimated in all the subjects. The AR activity was estimated by nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidation method. The plasma MDA levels were estimated by the thiobarbituric acid reactive substance (TBARS) method. For Statistical analysis, all the data were compared between the two groups by using unpaired t-test. Pearson correlation coefficient was calculated among T2DM. A P value <0.05 was considered as statistically significant for all data analyzed. The mean of FBS, PPBS, AR activity, and MDA levels were found significantly high in T2DM as compared to controls (P<0.001, P<0.001, P<0.001, P<0.001, respectively). A significant positive correlation was found between FBS and PPBS among T2DM (r=0.71, P<0.01). However, There was no significant correlation found between AR activity and MDA level among T2DM (r=0.002, P>0.05). Results showed thatthe mean of FBS, PPBS, AR activity, and MDA levels were found significantly higher in T2DM than controls. There was no significant correlation found between AR activity and MDA level among T2DM.

Download Full-text

PROGRAM EMAS DALAM KONTROL GULA DARAH PENDERITA DIABETES MELLITUS TIPE 2

J-Dinamika ◽

10.25047/j-dinamika.v5i1.1430 ◽

2020 ◽

Vol 5 (1) ◽

Author(s):

Arief Andriyanto ◽

Etty Rekawati ◽

Dwi Cahya Rahmadiyah

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Behavior Change ◽

Blood Sugar ◽

Communicable Disease ◽

Control Group ◽

P Value ◽

Nurse Specialist

Background: Diabetes mellitus is a non-communicable disease that is included in the category of chronic diseases and expected to experience an increase, so that a way to control is needed by the Ministry of Health in the form of clever management of diabetes mellitus. This study aimed to analyze the effect of EMAS (education, nutrition management, physical activity, stress management) on behavior change and adult blood sugar control with diabetes mellitus type 2. Methods: Quasi Experiment Pre-Post Test Without Control Group Design for 6 months, October 2018 to March 2019. Samples were taken by purposive sampling technique, namely adults with type 2 diabetes mellitus in Cisalak Pasar Sub-District Ciamnggis District, Depok City as many as 86 people. Results: Changes in behavior and control of adult blood sugar with type 2 diabetes mellitus through EMAS intervention (p value 0.001 <0.05). Conclusion: Changes in adult behavior with type 2 diabetes mellitus are needed to stabilize the patient's blood sugar. Therefore, it takes the role of the nurse specialist community to provide interventions according to the needs of people with diabetes mellitus to manage the disease.Keywords: Intervention EMAS; Behavior change; Glucose control; Type 2 diabetes mellitus

Download Full-text

Association of Serum Adipokines Levels with Glycemic Control and Metabolic Dyslipidemia in Sudanese Patients with Type 2 Diabetes Mellitus

10.21203/rs.3.rs-34969/v1 ◽

2020 ◽

Author(s):

Halima Babiikir Eltahir ◽

Elmahadi Mohamed Ali ◽

Abdelrahim Osman Mohamed

Keyword(s):

Diabetes Mellitus ◽

Insulin Resistance ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Adipose Tissue ◽

Glycemic Control ◽

Total Cholesterol ◽

Pearson Correlation ◽

P Value

Abstract Background:The pathogenesis of type 2 diabetes mellitus is due to two major abnormalities including insulin resistance and dysfunction, which lead to the inability to regulate blood glucose level. Adiponectin is a hormone secreted by the adipose tissue and it takes part in glucose metabolism with insulin-sensitising properties. Low levels of adiponectin leads to reduction of fatty acid oxidation decreased glucose uptake in skeletal muscle cells and increased level of free fatty acids leading to insulin resistance. Leptin is another adipokine produced by adipose tissue involved in the control of food intake via its action on the hypothalamus, suppressing appetite and stimulating energy expenditure. Leptin plays a critical role in pathophysiology of type 2 diabetes mellitus.The aim of the study was to investigate the association of serum adipokines levels with glycemic control and metabolic dyslipidemia in Sudanese patients with type 2 diabetes mellitus.Methods: This was a case control study. 202 patients with type 2 diabetes and 102 non-diabetic controls participated after signing written consent. Weight (kg) and height (m) were measured thenthe body mass index (kg/m2) was determined. Blood samples were collected after an overnight fasting. FBG, HbA1c and lipid profiles were measured using enzymatic methods. Adiponectin and leptin were measured using sandwich ELISA.Results: Adiponectin concentrations was significantly lower in patients with type 2 diabetes compared with the controls (p<0.001) and it was inversely correlated with HbA1c (Pearson Correlation -.160, P value = 0.005), total cholesterol and LDL levels (P = 0.05) and direct correlated HDL levels (P = 0.05). Leptin concentrations was significantly higher in patients with type 2 diabetes compared with the controls (p<0.002) and it was positively correlated with HbA1c (Pearson Correlation .155, P value = 0.02), total cholesterol and LDL levels (P = 0.05), there were no correlation with HDL and TG levels. Patients had significantly higher fasting blood glucose, HbA1c levels, total cholesterol and LDL levels compared with the controls. Conclusion: Patients with type 2 diabetes mellitus had decreased levels of serum adiponectin, high levels of serum leptin. There were significant correlations found between adiponectin and leptin levels with glycemic control and metabolic dyslipidemia

Download Full-text

The Effectiveness of Ma’tsurat Dhikr in Reducing Blood Sugar Levels in Type 2 Diabetes Mellitus Patients at Dr. Dradjat Prawiranegara Hospital Serang in 2018

KnE Life Sciences ◽

10.18502/kls.v4i13.5272 ◽

2019 ◽

Author(s):

Ernawati . ◽

Agus Sustiyono ◽

Mulyati .

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Blood Sugar ◽

Exercise Intervention ◽

Control Group ◽

P Value ◽

Glucose Levels ◽

Sugar Levels

Background: DM (Diabetes Mellitus) is a chronic/chronic metabolic disorder characterized by an increase in blood glucose levels (hyperglycemia) caused by insufﬁcient amounts of insulin or insufﬁcient amounts of insulin, sometimes even more effective, this condition is called insulin resistence. Objective: This study aims to determine the effectiveness of ma’tsurat dhikr therapy in reducing blood sugar levels in type 2 diabetes mellitus patients at dr. Dradjat Prawiranegara Hospital Serang in 2018. Methods: The number of samples of this study was 34 respondents and the method was quasi-experimental. The data was analyzed by independent T-Test. The respondents of experimental group received DM exercise and morning-evening dhikr intervention, while the control group only received DM exercise intervention. Results:The results of the study showed that ma’tsurat dhikr affects signiﬁcantly on the decrease of bloods ugarlevelsinpatienntswithtype2DMatdr.DradjatPrawiranegara Hospital Serang with p value<0.005. Conclusion: Intervention of ma’tsurat dhikr can be recommended for type 2 DM patients with elevated blood sugar levels in patients.

Download Full-text

Pengaruh Terapi Relaksasi Benson Terhadap Kadar Gula Darah Pada Penderita Diabetes Mellitus Tipe 2

Jurnal Penelitian Kesehatan SUARA FORIKES (Journal of Health Research Forikes Voice) ◽

10.33846/sf.v11i1.579 ◽

2019 ◽

Vol 11 (1) ◽

pp. 81

Author(s):

Putu Indah Sintya Dewi ◽

Ni Made Dwi Yunica Astriani ◽

I Made Sundayana ◽

Made Mahaguna Putra ◽

Ni Kadek Ita Ariani

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Health Center ◽

Blood Sugar ◽

T Test ◽

P Value ◽

Relaxation Therapy ◽

Sugar Levels

Benson relaxation is relaxation that combines relaxation respon techniques and belief system. This study aimed to analyze the effect of benson relaxation therapy on blood sugar levels in patiens with type 2 diabetes mellitus in the working area of Buleleng public health center III, utilized one group pretest-posttest design. The sample size was 40 respondents selected using nonprobability sampling technique. Data were collected using observation sheet, then alayzed using paired sample t-test. The result obtained p value of 0.001 (there was an effect of Benson Relaxation Therapy on Blood Sugar Levels in Patiens with Type 2 Diabetes Mellitus in the Working area of Buleleng Health Center III. Keywords: Benson relaxation; blood sugar ABSTRAK Relaksasi Benson merupakan relaksasi yang menggabungkan antara teknik respon relaksasi dan sistem keyakinan. Penelitian ini bertujuan menganalisis pengaruh terapi relaksasi benson terhadap kadar gula darah penderita diabetes mellitus tipe 2 di wilayah kerja Puskesmas Buleleng III, menggunakan one group pretest-posttest design. Besar sampel yang digunakan yaitu 40 responden yang dipilih dengan teknik nonprobability sampling. Data dikumpulkan dengan lembar observasi, lalu dianalisis dengan paired sampel t-test. Hasil mendapatkan nilai p = 0,001 (ada pengaruh terapi relaksasi benson terhadap kadar gula darah pada penderita diabetes mellitus tipe 2 di wilayah kerja Puskesmas Buleleng III. Kata kunci: relaksasi Benson; gula darah; diabetes

Download Full-text

Association of Serum Adipokines Levels with Glycemic Control and Metabolic Dyslipidemia in Sudanese Patients with Type 2 Diabetes Mellitus

10.21203/rs.3.rs-37122/v1 ◽

2020 ◽

Author(s):

Halima Babiikir Eltahir ◽

Elmahdi Mohamed Ali ◽

Abdelrahim Osman Mohamed

Keyword(s):

Diabetes Mellitus ◽

Insulin Resistance ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Adipose Tissue ◽

Glycemic Control ◽

Total Cholesterol ◽

Pearson Correlation ◽

P Value

Abstract Background: The pathogenesis of type 2 diabetes mellitus is due to two major abnormalities including insulin resistance and dysfunction, which lead to the inability to regulate blood glucose level. Adiponectin is a hormone secreted by the adipose tissue and it takes part in glucose metabolism with insulin-sensitising properties. Low levels of adiponectin leads to reduction of fatty acid oxidation decreased glucose uptake in skeletal muscle cells and increased level of free fatty acids leading to insulin resistance. Leptin is another adipokine produced by adipose tissue involved in the control of food intake via its action on the hypothalamus, suppressing appetite and stimulating energy expenditure. Leptin plays a critical role in pathophysiology of type 2 diabetes mellitus.The aim of the study was to investigate the association of serum adipokines levels with glycemic control and metabolic dyslipidemia in Sudanese patients with type 2 diabetes mellitus.Methods: This was a case control study. 202 patients with type 2 diabetes and 102 non-diabetic controls participated after signing written consent. Weight (kg) and height (m) were measured then the body mass index (kg/m2) was determined. Blood samples were collected after an overnight fasting. FBG, HbA1c and lipid profiles were measured using enzymatic methods. Adiponectin and leptin were measured using sandwich ELISA.Results: Adiponectin concentrations was significantly lower in patients with type 2 diabetes compared with the controls (p<0.001) and it was inversely correlated with HbA1c (Pearson Correlation -.160, P value = 0.005), total cholesterol and LDL levels (P = 0.05) and direct correlated HDL levels (P = 0.05). Leptin concentrations was significantly higher in patients with type 2 diabetes compared with the controls (p<0.002) and it was positively correlated with HbA1c (Pearson Correlation .155, P value = 0.02), total cholesterol and LDL levels (P = 0.05), there were no correlation with HDL and TG levels. Patients had significantly higher fasting blood glucose, HbA1c levels, total cholesterol and LDL levels compared with the controls.Conclusion: Patients with type 2 diabetes mellitus had decreased levels of serum adiponectin, high levels of serum leptin. There were significant correlations found between adiponectin and leptin levels with glycemic control and metabolic dyslipidemia .

Download Full-text

RELAKSASI AUTOGENIK MENURUNKAN KADAR GULA DARAH PASIEN DIABETES MELITUS TIPE 2

Real in Nursing Journal ◽

10.32883/rnj.v1i3.475 ◽

2018 ◽

Vol 1 (3) ◽

pp. 133

Author(s):

Aria Wahyuni ◽

Imelda Rahmayunia Kartika ◽

Amira Pratiwi

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Blood Sugar ◽

Blood Sugar Level ◽

Bivariate Analysis ◽

P Value ◽

Type 2 Dm ◽

Autogenic Relaxation

Diabetes Mellitus (DM) is a group of metabolic diseases characterized by hyperglycemia resulting from a lack of insulin secretion, insulin action, or both. The aim of the study was to determine the effect of autogenic relaxation on the reduction of blood sugar levels in Type 2 Diabetes Mellitus Patients. The research design used was Quasi Experimental with the Pretest-Posttest approach method. This research was conducted in one of the work areas of the health center in the city of Bukittinggi in June-July 2108. The population of this study was all patients with type 2 DM totaling 150 people while the samples taken were 15 people with a purposive sampling technique. The analysis used is univariate and bivariate analysis using the dependent t-test. The results of this study found that the average blood sugar level before the autogenic relaxation intervention was 214.4 gr/dl, the average blood sugar level after autogenic relaxation was 205 gr/dl with a p value = 0,000 meaning that there was an effect of autogenic relaxation on decreasing levels blood sugar in type 2 DM patients. The recommendation of this study is to continue this study with a large sample and with long and periodic interventions. Keywords: Type 2 Diabetes Mellitus, Blood Sugar Level, Autogenic Relaxation

Download Full-text

Pengaruh Terapi Relaksasi Benson Terhadap Kadar Gula Darah Pada Penderita Diabetes Mellitus Tipe 2

Jurnal Penelitian Kesehatan SUARA FORIKES (Journal of Health Research Forikes Voice) ◽

10.33846/sf11117 ◽

2019 ◽

Vol 11 (1) ◽

pp. 81

Author(s):

Putu Indah Sintya Dewi ◽

Ni Made Dwi Yunica Astriani ◽

I Made Sundayana ◽

Made Mahaguna Putra ◽

Ni Kadek Ita Ariani

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Health Center ◽

Blood Sugar ◽

T Test ◽

P Value ◽

Relaxation Therapy ◽

Sugar Levels

Download Full-text

SITAGLIPTIN;

The Professional Medical Journal ◽

10.29309/tpmj/2017.24.11.675 ◽

2017 ◽

Vol 24 (11) ◽

pp. 1755-1760

Author(s):

Zaheer Ahmed ◽

Hassan Fareed ◽

Muhammad Usman Musharraf

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Blood Sugar ◽

T Test ◽

P Value ◽

Diabetic Patients ◽

Group A ◽

Group B

The objective of this stidy is to compare the mean reduction of HbA1c in patientstaking sitagliptin insulin combination with insulin alone in patients of type II Diabetes mellitus.Study Design: Randomized control trial (RCT). Setting: Medicine Department, Allied Hospital,Faisalabad. Period: August 2013 to February 2014. Methodology: Patients of both genderswith ages between 18 and 60 years having uncontrolled type 2 diabetes mellitus with HbA1ctaking insulin were included in the study while Pregnant or lactating mothers, patients withchronic liver disease and patients with renal insufficiency (creatinine >3.0mg/dl) were excludedfrom study. Patients were randomly divided into two groups (A &B) using computer generatedrandom number table. Group A was given Sitagliptin 50mg per day and dose was increasedto 100mg per day after 2 weeks if fasting blood sugar was more than 110mg/dl or 2 hourspostprandial blood sugar more than 140mg/dl. Dose of insulin in group A remained same asbefore start of study. Group B was kept on same regimen of insulin they were already taking anddose remained same. HbA1c was done at start of study and after 24 weeks. Primary outcomemeasure was mean reduction in HbA1c levels at 24 weeks from baseline. Results: 60 patientswere included in the study. Mean age was 52.7+8.43 years. 36(60%) were male and 24(40%)were female. Mean HbA1c at baseline was 8.361+0.523% in Group A on Sitagliptin-insulincombination and 8.187+0.432% in group B on insulin alone. Mean HbA1c at 24 weeks was7.767+0.428% in group A on Sitagliptin-insulin combination and 7.69+0.407% in group B oninsulin alone.Independent sample t-test was applied to change in HbA1c in Group A and Bduring 24 weeks of treatment. Mean change in HbA1c after 24 weeks in Group A was 0.600+ 0.315 and 0.49+ 0.19 in Group B after 24 weeks treatment (p-value 0.002) which is highlysignificant. Paired sample t-test was applied to HbA1c at baseline and 24 weeks of treatmentin group A and group B. There was a significant change in both groups after 24 weeks oftreatment in both groups (p-value <0 .000) which is highly significant. Chi Square test wasapplied on efficacy in group A and B. 22(73.33%) patients in group A and 14(46.67%) patientsin group B which achieved significant reduction in HbA1c. 8(26.67%) patients in group A and16(53.33%) patients in group B failed to achieve significant reduction in HbA1c. (P-value <0.032). Conclusion: It has been concluded from this study that insulin and sitagliptin-insulincombination both significantly reduce HbA1c in type 2 diabetes mellitus. However addition ofsitagliptin to uncontrolled diabetic patients already taking insulin, is more effective than insulinfor glycemic control of patients with type 2 diabetes mellitus.

Download Full-text

Magnesium status in a population of type 2 diabetes mellitus in Morocco

Endocrinology&Metabolism International Journal ◽

10.15406/emij.2021.09.00303 ◽

2021 ◽

Vol 9 (1) ◽

pp. 20-23

Author(s):

Wafa Quiddi ◽

M Tarmidi ◽

A Mansouri ◽

M Amine ◽

H Baizri ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Linear Relationship ◽

Blood Sugar ◽

Pearson Correlation ◽

Fasting Blood Sugar ◽

Military Hospital ◽

Negative Linear Relationship

Background: Type 2 diabetes mellitus (T2DM) contributes to 90% cases of diabetes–the ninth leading cause of death. Magnesium (Mg) levels have been found consistently low in the patients with T2DM around the world. Objective: The objective of our work was to assess the Mg status in the patients with T2DM. Material and methods: A descriptive prospective study including 103 patients with type 2 diabetes mellitus (T2DM) was carried out at Avicenna Military Hospital in Marrakesh over a period of four months (October 2019 - January 2020). The patients with T2DM and age above 35years with no history of Mg supplementation, cancer or autoimmune disease were included in the study. Blood samples were analyzed for fasting glucose, lipid profile, HbA1c, and serum magnesium. The data were analyzed by SPSS version 16, Pearson correlation was performed to establish the relationship between Mg and metabolic variables in T2DM patients. Results: The study included 103 T2DM patients with 56 (54.4%) males and 47 (45.6%) females. Prevalence of hypomagnesemia was 14.56%. Mean age of the patients and duration of diabetes were 59±9 years and 9±4years, respectively. Most common antecedent conditions were dyslipidemia (43.7%), hypertension (42.7%), and retinopathy (25.2%). Hypomagnesemia was significantly associated with HbA1c (P=0.003) and arterial hypertension (P=0.002). Magnesium had negative linear relationship with increased fasting blood sugar and HbA1c. Conclusion: In Morocco, the prevalence of hypomagnesemia in T2DM patient is low, but it has negative linear relationship with fasting blood sugar and HbA1c.

Download Full-text

ESTIMATION OF SERUM FERRITIN LEVELS IN TYPE 2 DIABETIC PATIENTS AND ITS RELATION WITH HbA1C LEVEL.

International Journal of Medical and Biomedical Studies ◽

10.32553/ijmbs.v3i8.456 ◽

2019 ◽

Vol 3 (8) ◽

Author(s):

Shah Namrata Vinubhai ◽

Pardeep Agarwal ◽

Bushra Fiza ◽

Ramkishan Jat

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Blood Sugar ◽

Serum Ferritin ◽

Inflammatory Marker ◽

Control Group ◽

Diabetic Patients ◽

Positive Correlation

Background: Serum ferritin is known as an index for body iron stores also as an inflammatory marker and it is influenced by several disease. We were looking for a correlation between HbA1c and S. Ferritin in type 2 DM. Methodology: The present study a total of 150 participants were enrolled of which 100 were confirmed cases of Type 2 Diabetes Mellitus and rest 50 age and sex matched healthy subjects constituted the control group. All were screened for HbA1c, Fasting blood sugar, Post prandial blood sugar and S.Ferritin. Results: A highly significant variation and positive correlation was observed with respect to S.Ferritin and HbA1c levels. Mean S.Ferritin was high in the subgroup with poor glycemic control. Conclusion: The fasting, post prandial sugar levels, HbA1c and S.Ferritin were significantly higher in the diabetic subjects. This study shows a positive correlation between HbA1c and S. Ferritin levels. So we can conclude that in diabetic patients S. Ferritin may serve as an independent marker of poor glycemic and metabolic control. Keywords: Serum ferritin, Type 2 Diabetes Mellitus, HbA1c.

Download Full-text