The Effect of Fibronectin-Immobilized Microgrooved Titanium Substrata on Cell Proliferation and Expression of Genes and Proteins in Human Gingival Fibroblasts

Zirconia exhibits excellent biocompatibility and is widely used as dental implant materials in prosthodontics. Over the past years, research and development of dental implant biomaterials has focused on osseointegration, but few reports exist regarding the role of the immune environment on cellular responses to these materials. The present study investigates the effect of different nanostructured zirconia surface topographies on macrophage phenotypes and their influence on gingival fibroblast behavior. Three different nanostructured zirconia surfaces are characterized using scanning electron microscopy, atomic force microscopy, and water contact angle. Blank-machined zirconia (BMZ) surfaces were superior to RAW264.7 cell proliferation and adhesion. RAW264.7 seeded on all nanostructured zirconia surfaces polarized toward both inflammatory M1 and anti-inflammatory M2 macrophages with more M2 macrophage phenotype on BMZ surfaces. Meanwhile, conditioned media (CM) from RAW264.7 culture on three nanostructured zirconia surfaces inhibited cell apoptosis to human gingival fibroblasts (HGFs) but promoted HGF proliferation and secretion. Under modulation of RAW264.7 culture, HGFs cultured on BMZ surfaces significantly secreted more extracellular matrix with a higher expression of collagen-I (COL-I), vinculin (VCL), and fibronectin (FN) than those coated on self-glazed zirconia (CSGZ) and self-glazed zirconia (SGZ) surfaces. After being coated with a nano zirconia film, CSGZ surfaces showed certain changes in cell proliferation, adhesion, and protein production compared with SGZ surfaces. These findings will provide an overview of manipulating surface topography to modulate macrophage phenotypes in order to create an effective macrophage immune response and reinforce soft tissue integration.

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Effect of Nd:YAG Low Level Laser Therapy on Human Gingival Fibroblasts

International Journal of Dentistry ◽

10.1155/2015/258941 ◽

2015 ◽

Vol 2015 ◽

pp. 1-7 ◽

Cited By ~ 8

Author(s):

Andreas S. Gkogkos ◽

Ioannis K. Karoussis ◽

Ioannis D. Prevezanos ◽

Kleopatra E. Marcopoulou ◽

Kyriaki Kyriakidou ◽

...

Keyword(s):

Cell Proliferation ◽

Growth Factors ◽

Laser Irradiation ◽

Laser Therapy ◽

Dose Response ◽

Statistical Significance ◽

Human Gingival Fibroblasts ◽

Low Level Laser Therapy ◽

Gingival Fibroblasts ◽

Level Laser

Aim. To evaluate the effect of Low Level Laser Therapy (LLLT) on human gingival fibroblasts in terms of proliferation and growth factors’ secretion (EGF, bFGF, and VEGF).Materials and Methods. Primary cultures of keratinized mucosa fibroblasts were irradiated by a Nd:YAG laser 1064 nm with the following energy densities: 2.6 J/cm2, 5.3 J/cm2, 7.9 J/cm2, and 15.8 J/cm2. Controls were not irradiated. Cultures were examined for cell proliferation and growth factors’ secretion after 24, 48, and 72 hours. All experimental procedures were performed in duplicate. Data were analyzed by Student’st-test (p<0.05).Results. All laser-irradiation doses applied promoted a higher cell proliferation at 48 hours in a dose-response relationship compared to controls. This difference reached statistical significance for the cultures receiving 15.8 J/cm2(p=0.03). Regarding EGF, all laser irradiation doses applied promoted a higher secretion at 48 hours in a reverse dose-response pattern compared to controls. This difference reached statistical significance for the cultures receiving 2.6 J/cm2(p=0.04). EGF levels at the other time points, bFGF, and VEGF showed a random variation between the groups.Conclusion. Within the limits of this study, LLLT (Nd:YAG) may induce gingival fibroblasts’ proliferation and upregulate the secretion of EGF. Further studies are needed to confirm these results.

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Tenidap, an Anti-Inflammatory Agent, Inhibits DNA and Collagen Syntheses, Depresses Cell Proliferation, and Lowers Intracellular pH in Cultured Human Gingival Fibroblasts

Journal of Pharmacology and Experimental Therapeutics ◽

10.1124/jpet.300.2.668 ◽

2002 ◽

Vol 300 (2) ◽

pp. 668-672 ◽

Cited By ~ 10

Author(s):

Hiroko Matsumoto ◽

Akira Fujii

Keyword(s):

Cell Proliferation ◽

Intracellular Ph ◽

Human Gingival Fibroblasts ◽

Gingival Fibroblasts ◽

Inflammatory Agent ◽

Anti Inflammatory

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Comparative Evaluation of the Effects of Antimicrobial Photodynamic Therapy With an LED and a Laser on the Proliferation of Human Gingival Fibroblasts on the Root Surface: An In Vitro Study

Journal of lasers in medical sciences ◽

10.34172/jlms.2021.47 ◽

2021 ◽

Vol 12 (1) ◽

pp. e47-e47

Author(s):

Mahsa Koochaki ◽

Amirreza Hendi ◽

Mahmood Ghasemi ◽

Ehsan Seyedjafari ◽

Mehdi Hamidain ◽

...

Keyword(s):

Cell Proliferation ◽

Photodynamic Therapy ◽

Citric Acid ◽

Repeated Measures ◽

In Vitro Study ◽

Acid Group ◽

Human Gingival Fibroblasts ◽

Antimicrobial Photodynamic Therapy ◽

Gingival Fibroblasts

Introduction: This study aimed to compare the effects of root biomodification by citric acid and antimicrobial photodynamic therapy (aPDT) with LED and laser on the proliferation of human gingival fibroblasts (HGFs). Methods: This in vitro experimental study evaluated 60 single-rooted teeth extracted due to periodontal disease. The teeth underwent scaling and root planing (SRP), and then 5 × 5 mm blocks were prepared from the cervical area of the teeth 1 mm apical to the cementoenamel junction. The blocks were divided into 4 groups (n=15 blocks): SRP alone (control), SRP + citric acid, SRP + toluidine blue (TBO) + LED light, and SRP + TBO + laser. HGFs were seeded on the surface of the samples, and the methyl thiazolyl tetrazolium (MTT) assay was performed after 24, 48 and 72 hours. Group comparisons were performed using repeated measures ANOVA, while pairwise comparisons of the time points were performed by an LSD test. Results: Cell proliferation was higher in all experimental groups at 48 and 72 hours, compared with 24 hours (P<0.05). Cell proliferation was significantly different in the citric acid group at 24 hours (P=0.016) and 48 hours (P=0.015), compared with other groups. However, cell proliferation was not significantly different in the aPDT group with LED Photosan and a diode laser at 24 and 48 hours (P>0.05). Conclusion: aPDT and citric acid can enhance the proliferation of HGFs on dentin blocks. Further studies can pave the way for their future use in the clinical setting.

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