Isolation of Borrelia burgdorferi sensu lato from blood of adult patients with borrelial lymphocytoma, Lyme neuroborreliosis, Lyme arthritis and acrodermatitis chronica atrophicans

Infection ◽  
2010 ◽  
Vol 39 (1) ◽  
pp. 35-40 ◽  
Author(s):  
V. Maraspin ◽  
K. Ogrinc ◽  
E. Ružić-Sabljić ◽  
S. Lotrič-Furlan ◽  
F. Strle
Keyword(s):  
Borrelia Burgdorferi ◽  
Lyme Neuroborreliosis ◽  
Adult Patients ◽  
Lyme Arthritis ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Acrodermatitis Chronica Atrophicans

scholarly journals Clinical Features of 705Borrelia burgdorferiSeropositive Patients in an Endemic Area of Northern Italy

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Giuseppe Stinco ◽  
Maurizio Ruscio ◽  
Serena Bergamo ◽  
Davide Trotter ◽  
Pasquale Patrone
Keyword(s):  
Borrelia Burgdorferi ◽  
Clinical Features ◽  
Lyme Borreliosis ◽  
Erythema Migrans ◽  
Geographic Area ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Neurological Involvement ◽  
Clinical Feature ◽  
Cutaneous Manifestations ◽  
Acrodermatitis Chronica Atrophicans

Background. Lyme Borreliosis is a multisystemic infection caused by spirochetes ofBorrelia burgdorferi sensu latocomplex. The features of Lyme Borreliosis may differ in the various geographical areas, primarily between the manifestations found in America and those found in Europe and Asia.Objective. to describe the clinical features of Lyme Borreliosis in an endemic geographic area such as Friuli-Venezia Giulia in the Northeastern part of Italy.Methods. The medical records of patients resulted seropositive forBorrelia burgdorferihave been retrospectively recorded and analyzed.Results. Seven hundred and five patients met the inclusion criteria, 363 males and 342 females. Erythema migrans was the most common manifestation, detected in 437 patients. Other classical cutaneous manifestations included 58 cases of multiple erythema migrans, 7 lymphadenosis benigna cutis, and 18 acrodermatitis chronica atrophicans. The musculoskeletal system was involved in 511 patients. Four hundred and sixty patients presented a neurological involvement. Flu-like symptoms preceded or accompanied or were the only clinical feature in 119 patients.Comments. The manifestations of Lyme borreliosis recorded in this study are similar to the ones of other endemic areas in Europe, even if there are some peculiar features which are different from those reported in Northern Europe and in the USA.

scholarly journals Lyme neuroborreliosis in Swedish children—PCR as a complementary diagnostic method for detection of Borrelia burgdorferi sensu lato in cerebrospinal fluid

2021 ◽  
Author(s):  
Barbro H. Skogman ◽  
Peter Wilhelmsson ◽  
Stephanie Atallah ◽  
Ann-Cathrine Petersson ◽  
Katarina Ornstein ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Neurological Disorders ◽  
Diagnostic Method ◽  
Lyme Neuroborreliosis ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Rrna Gene ◽  
Antibody Index ◽  
Chain Reaction ◽  
Serum Samples ◽  
Polymerase Chain

AbstractThe aim of this study was to evaluate polymerase chain reaction (PCR) as a diagnostic method for the detection of Borrelia burgdorferi s.l. in CSF of Swedish children with LNB. This study was performed retrospectively on CSF and serum samples collected from children evaluated for LNB (n = 233) and controls with other specific neurological disorders (n = 59) in a Swedish Lyme endemic area. For anti-Borrelia antibody index, the IDEIA Lyme Neuroborreliosis kit (Oxoid) was used. Two in-house real-time PCR assays targeting the 16S rRNA gene were evaluated (TaqMan® and LUX™). Among patients classified as LNB cases (n = 102), five children (5%) were Borrelia PCR-positive in CSF with the TaqMan® assay. In the Non-LNB group (n = 131), one patient was Borrelia PCR positive with the TaqMan® assay. Among controls (n = 59), all CSF samples were PCR negative. When amplifying and sequencing ospA, we found B. garinii (n = 2), B. afzelii (n = 2), B. bavariensis (n = 1), and one untypable (n = 1). With the LUX™ technology, all CSF samples were PCR negative. The TaqMan® assay could detect only few cases (n = 6) of B. burgdorferi s.l. in CSF among children with LNB and the sensitivity was very low (5%). However, using larger CSF volumes and centrifugation of samples, the PCR technique could still be useful as a complementary diagnostic method when evaluating LNB. Furthermore, detection of spirochete DNA in clinical matrices, including CSF, is the method of choice for studying epidemiological aspects of LNB, a tick-borne emerging disease.

Lyme-Arthritis bei Kindern

2011 ◽  
Vol 31 (02) ◽  
pp. 129-134 ◽  
Author(s):  
H.-I. Huppertz ◽  
F. Dressler
Keyword(s):  
Borrelia Burgdorferi ◽  
Lyme Arthritis ◽  
Borrelia Burgdorferi Sensu Lato

ZusammenfassungDie Lyme-Arthritis ist durch die Infektion mit Borrelia burgdorferi sensu lato verursacht. In dieser Arbeit wird der aktuelle Wissensstand zu Epidemiologie, Mikrobiologie, Pathogenese sowie zu den klinischen Manifestationen und deren Diagnose, Behandlung und Prognose dargestellt, besonders bezogen auf das Kindes- und Jugendalter.

scholarly journals 64Cu-DOTATATE Positron Emission Tomography (PET) of Borrelia Burgdorferi Infection: In Vivo Imaging of Macrophages in Experimental Model of Lyme Arthritis

Diagnostics ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 790
Author(s):  
Anne Skovsbo Clausen ◽  
Mathilde Ørbæk ◽  
Regitze Renee Pedersen ◽  
Peter Oestrup Jensen ◽  
Anne-Mette Lebech ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Plasma Levels ◽  
Lyme Arthritis ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Late Time ◽  
Macrophage Marker ◽  
Positron Emission ◽  
Pet Ct ◽  
Significant Difference

Macrophages play a key role in the inflammatory response in Lyme arthritis (LA) and could be a target for diagnosing and monitoring active Borrelia burgdorferi sensu lato (Bb) infection. Therefore, we evaluated the potential of macrophage imaging using 64Cu-DOTATATE PET/CT for detection of Bb activity in a murine model of LA. LA was established in C3H/HeNRj mice infected with Bb B31 strain ML23 pBBE22luc. Bioluminescence imaging was performed to detect migration of spirochetes and inflammatory phagocytes to the joints. Three weeks post-infection 64Cu-DOTATATE PET/CT imaging was performed at an early (3 h) and late (48 h) time point. Plasma levels of a systemic macrophage marker in plasma CD163 were measured. 64Cu-DOTATATE uptake in infected joints was increased at the early (p < 0.0001) and late time points (p = 0.0005) compared with uptake in non-infected controls. No significant difference in plasma levels of CD163 was measured. 64Cu-DOTATATE PET allows for in vivo detection and quantification of LA locally in the joints through non-invasive visualization of macrophages. In contrast, measurement of a systemic macrophage marker in plasma, CD163, did not allow to detect disease. We suggest that 64Cu-DOTATATE PET could become a valuable diagnostic tool for in situ detection of Bb infection-related inflammation.

scholarly journals Neurological symptoms caused by the subspecies borrelia burgdorferi

2001 ◽  
Vol XXXIII (1-2) ◽  
pp. 53-55
Author(s):  
A. Haass
Keyword(s):  
Borrelia Burgdorferi ◽  
Clinical Symptoms ◽  
Epidemiological Studies ◽  
Sensu Stricto ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Acrodermatitis Chronica Atrophicans ◽  
Number Of Patients ◽  
Skin Erythema ◽  
The Difference ◽  
Tick Vectors

Borreliosis is characterized by a variety of clinical symptoms affecting the skin (erythema chronicum migrans, lymphadenopathia cutis benigna, acrodermatitis chronica atrophicans), eyes (conjunctivitis, iritis, uveitis), muscles (myositis), nervous system (neuritis, radiculitis, polyradiculitis, meningopolyralitis ), joints (arthritis, arthralgia) and heart (carditis). The variety of symptoms is explained by the difference in the subspecies Borrelia burgdorferi sensu lato in virulence and organotropism and changes in the patient's immune response. The main subspecies Borrelia burgdorferi sensu lato (B.b.s.l.) are Borrelia burgdorferi sensu stricto (B.b.s.s.), Borrelia burgdorferi garinii (B.b.g.) and Borrelia burgdorferi afzelii (B.b.a.). In North America, only B.b.s.s. has been found, while all 3 species can be found in Europe. Epidemiological studies have shown that the number of patients with borreliosis is still increasing, which is associated with an increase in the density of distribution of tick vectors, which are carriers of the disease, and with the degree of awareness of people.

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