Insertion Sequence Based Molecular Characterization of Burkholderia mallei NCTC 3709 Strain

2014 ◽  
Vol 38 (2) ◽  
pp. 99-102
Author(s):  
Chandan Prakash ◽  
P. Das ◽  
B. V. Sunil Kumar ◽  
Bincy Joseph ◽  
Vidya Singh ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Insertion Sequence ◽  
Burkholderia Mallei

scholarly journals Clinical and Molecular Characterization of a Novel Selenocysteine Insertion Sequence-Binding Protein 2 (SBP2) Gene Mutation (R128X)

2009 ◽  
Vol 94 (10) ◽  
pp. 4003-4009 ◽  
Author(s):  
Caterina Di Cosmo ◽  
Neil McLellan ◽  
Xiao-Hui Liao ◽  
Kum Kum Khanna ◽  
Roy E. Weiss ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Gene Mutation ◽  
Insertion Sequence ◽  
Binding Protein

scholarly journals Serotype 1a O-Antigen Modification: Molecular Characterization of the Genes Involved and Their Novel Organization in the Shigella flexneriChromosome

1999 ◽  
Vol 181 (15) ◽  
pp. 4711-4718 ◽  
Author(s):  
Pradip Adhikari ◽  
Gwen Allison ◽  
Belinda Whittle ◽  
Naresh K. Verma
Keyword(s):  
Sequence Analysis ◽  
Molecular Characterization ◽  
Insertion Sequence ◽  
Shigella Flexneri ◽  
Pathogenicity Islands ◽  
Is Elements ◽  
O Antigen ◽  
Phage Dna

ABSTRACT The factors responsible for serotype 1a O-antigen modification inShigella flexneri were localized to a 5.8-kb chromosomalHindIII fragment of serotype 1a strain Y53. The entire 5.8-kb fragment and regions up- and downstream of it (10.6-kb total) were sequenced. A putative three-gene operon, which showed homology with other serotype conversion genes, was identified and shown to confer serotype 1a O-antigen modification. The serotype conversion genes were flanked on either side by phage DNA. Multiple insertion sequence (IS) elements were located within and upstream of the phage DNA in a composite transposon-like structure. Host DNA homologous to the dsdC and the thrW proA genes was located upstream of the IS elements and downstream of the phage DNA, respectively. The sequence analysis indicates that the organization of the 10.6-kb region of the Y53 chromosome is unique and suggests that the serotype conversion genes were originally brought into the host by a bacteriophage. Several features of this region are also characteristic of pathogenicity islands.

1446: Molecular Characterization of Cyclooxygenase-2 in a Stimulated Prostate Stroma Cells

2006 ◽  
Vol 175 (4S) ◽  
pp. 467-467
Author(s):  
Victor K. Lin ◽  
Shih-Ya Wang ◽  
Claus G. Roehrbom
Keyword(s):  
Molecular Characterization ◽  
Cyclooxygenase 2 ◽  
Prostate Stroma
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