Prevalence, Serogroups, Shiga-toxin Genes and Pulsed Field Gel Electrophoresis Analyses of Escherichia coli Isolated from Bovine Milk

2013 ◽  
Vol 83 (3) ◽  
pp. 423-429 ◽  
Author(s):  
Dilecta D’Costa ◽  
Saroj N. Bhosle ◽  
R. B. Dhuri ◽  
S. P. Doijad ◽  
K. V. Poharkar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Gel Electrophoresis ◽  
Bovine Milk ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Toxin Genes ◽  
Shiga Toxin Genes

scholarly journals Produce Isolates of the Escherichia coli Ont:H52 Serotype That Carry both Shiga Toxin 1 and Stable Toxin Genes

2006 ◽  
Vol 72 (4) ◽  
pp. 3062-3065 ◽  
Author(s):  
Steven R. Monday ◽  
Christina Keys ◽  
Patricia Hanson ◽  
Yuelian Shen ◽  
Thomas S. Whittam ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Single Nucleotide Polymorphism ◽  
Shiga Toxin ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Pulsed Field ◽  
Clonal Group ◽  
Toxin Genes

ABSTRACT Produce isolates of the Escherichia coli Ont:H52 serotype carried Shiga toxin 1 and stable toxin genes but only expressed Stx1. These strains had pulsed-field gel electrophoresis profiles that were 90% homologous to clinical Ont:H52 strains that had identical phenotypes and genotypes. All Ont:H52 strains had identical single nucleotide polymorphism profiles that are suggestive of a unique clonal group.

scholarly journals Longitudinal Study of Shiga Toxin-Producing Escherichia coli Shedding in Sheep Feces: Persistence of Specific Clones in Sheep Flocks

2009 ◽  
Vol 75 (6) ◽  
pp. 1769-1773 ◽  
Author(s):  
Sergio Sánchez ◽  
Remigio Martínez ◽  
Alfredo García ◽  
Jorge Blanco ◽  
Jesús E. Blanco ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Longitudinal Study ◽  
Shiga Toxin ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Individual Animal ◽  
Pulsed Field ◽  
The Individual ◽  
Sheep Feces

ABSTRACT To provide information on the persistence and maintenance of colonization with Shiga toxin-producing Escherichia coli (STEC) in sheep, pulsed-field gel electrophoresis analysis of STEC isolates (n = 145) belonging to serogroups O5, O91, and O146 from 39 healthy animals was performed in a 12-month longitudinal study carried out with four sheep flocks. At the flock level as well as the individual-animal level, the same clones were obtained on sampling occasions separated by as much as 11 months.

Pulsed-Field Gel Electrophoresis Characterization of Shiga Toxin–Producing Escherichia coli O157 from Hides of Cattle at Slaughter

2002 ◽  
Vol 65 (7) ◽  
pp. 1172-1176 ◽  
Author(s):  
S. M. AVERY ◽  
A. SMALL ◽  
C.-A. REID ◽  
S. BUNCIC
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Gel Electrophoresis ◽  
Immunomagnetic Separation ◽  
Pulsed Field Gel Electrophoresis ◽  
Escherichia Coli O157 ◽  
Adult Cattle ◽  
Pulsed Field ◽  
E Coli ◽  
High Prevalence

Contamination of the brisket areas of the hides of healthy adult cattle with Shiga toxin–producing Escherichia coli O157 at slaughter in England was studied. In total, 73 cattle consignments comprising 584 animals delivered to one abattoir over 3 days during 1 week in July 2001 were studied: 26 cattle consignments arriving on Monday, 32 consignments arriving on Wednesday, and 15 consignments arriving on Friday. Consignment sizes ranged from 1 to 23 animals, with a mean consignment size of 8. The hide of the first animal to be slaughtered in each consignment was sampled by using a sponge swab moistened with 0.85% saline to rub an unmeasured brisket (ventral) area (ca. 30 by 30 cm). The process of isolating E. coli O157 from the swabs consisted of enrichment, screening with immunoprecipitation assay kits, and immunomagnetic separation. E. coli O157 was found on 24 of 73 (32.9%) cattle hides examined, and 21 of these 24 isolates produced Shiga toxins. The 24 E. coli O157 isolates produced six different pulsed-field gel electrophoresis profiles, and 18 (75%) of the isolates were of one prevalent clone. The high prevalence of one E. coli O157 clone on the hides of cattle at slaughter could be due to a high prevalence of that clone on the 18 farms involved (not investigated in the current study), in the postfarm transport or lairage environments, or both. Since the lairage environment, but not the farm of origin or the postfarm transport vehicle, was a factor common to all 18 cattle consignments, it could have played an important role in spreading the prevalent E. coli O157 clone to the cattle hides. Lairage pen floors and the stunning box floor were identified as the most probable sites along the unloading-to-slaughter route at which the brisket areas of cattle hides could become contaminated.

Prevalence and Characterization of Escherichia coli O157:H7 on Pork Carcasses and in Swine Colon Contents from Provincially Licensed Abattoirs in Alberta, Canada

2020 ◽  
Vol 83 (11) ◽  
pp. 1909-1917
Author(s):  
SAIDA ESSENDOUBI ◽  
XIANQIN YANG ◽  
ROBIN KING ◽  
JULIA KEENLISIDE ◽  
JAVIER BAHAMON ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Gel Electrophoresis ◽  
Mean Value ◽  
Pulsed Field Gel Electrophoresis ◽  
Escherichia Coli O157 ◽  
Pulsed Field ◽  
E Coli ◽  
Swine Farms

ABSTRACT The objective of this study was to determine the prevalence of Shiga toxin–producing Escherichia coli (STEC) O157:H7 in colon contents and on carcasses from pigs slaughtered at provincially licensed abattoirs (PLAs) in Alberta, Canada. In 2017, carcass sponge samples and colon content samples were collected from 504 healthy market hogs at 39 PLAs and analyzed for E. coli O157:H7. Carcass samples were also analyzed for E. coli and aerobic colony count (ACC). Nine (1.8%) of 504 carcass samples were confirmed positive for E. coli O157:H7. Seven (1.4%) of 504 colon content samples were confirmed positive for E. coli O157:H7. These positives were found in 5 (12.8%) of 39 PLAs from hogs originating from eight farms. The E. coli O157:H7 isolates recovered from the positive samples (n = 1 isolate per sample) were clonal, as determined by pulsed-field gel electrophoresis. Six E. coli O157:H7 isolates obtained over 8 months from one PLA that only processed hogs and sourced hogs from one farm had indistinguishable pulsed-field gel electrophoresis patterns. All 16 E. coli O157:H7 isolates harbored eae and ehxA and were of stx2a subtype, suggesting that swine can carry E. coli O157:H7 of importance to human health. All carcass sponge swabs (100%) were positive for ACC. E. coli was present in 72% of carcass swabs. Carcasses from PLAs slaughtering both beef and hogs had a numerically higher ACC mean value but not statistically different compared with the carcasses from PLAs slaughtering only swine (2,799 and 610 CFU/cm2, respectively). E. coli showed a similar trend with a mean value of 0.88 CFU/cm2 in PLAs slaughtering both species and 0.26 CFU/cm2 in PLAs slaughtering only swine (P ≤ 0.05). This study provides evidence that healthy market hogs from different producers and farms in Alberta can carry E. coli O157:H7, and some strains of the organism may be able to establish persistence on some swine farms. HIGHLIGHTS

Effects of Lysogeny of Shiga Toxin 2-Encoding Bacteriophages on Pulsed-Field Gel Electrophoresis Fragment Pattern of Escherichia coli K-12

2003 ◽  
Vol 46 (3) ◽  
pp. 224-227 ◽  
Author(s):  
Atsushi Iguchi ◽  
Ro Osawa ◽  
Junichi Kawano ◽  
Akira Shimizu ◽  
Jun Terajima ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Fragment Pattern ◽  
Shiga Toxin 2 ◽  
K 12
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