Influence of the off line heating treatment parameter on the microstructure of a high strength steel plate was studied system previously. However, the information about the effect of on-line heating treatment process on ultra-high steel plate, especially on the plate with a tensile strength 980MPa or above, is limited due to the lack of effective method to simulation for the on-line heating treatment process. A method, which is preformed with a thermo-mechanical simulator, simulation the parameter of on-line heating treatment on the microstructure evolution a high strength ULCB steel plate after the accelerated cooling. By means of observation the original microstructure and microstructure morphologies varied with the parameter of the on-line heating treatment process, the present results demonstrate the microstructure characterizations before and after heating treatment process, while also the distribution of M(C, N) particles on lath boundaries or lath interior and the volume fraction and the average size of M/A island.