Identification of proteins in response to terminal drought stress in sorghum (Sorghum bicolor (L.) Moench) using two-dimensional gel-electrophoresis and MALDI-TOF-TOF MS/MS

2018 ◽  
Vol 23 (1) ◽  
pp. 24-39 ◽  
Author(s):  
Adugna Abdi Woldesemayat ◽  
David M. Modise ◽  
Bongani K. Ndimba
Keyword(s):  
Drought Stress ◽  
Sorghum Bicolor ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Maldi Tof ◽  
Terminal Drought ◽  
Terminal Drought Stress ◽  
Sorghum Bicolor L ◽  
Tof Ms

Proteomic analysis of human very low-density lipoprotein by two-dimensional gel electrophoresis and MALDI-TOF/TOF

PROTEOMICS ◽  
2007 ◽  
Vol 7 (1) ◽  
pp. 143-154 ◽  
Author(s):  
Carmine Mancone ◽  
Laura Amicone ◽  
Gian Maria Fimia ◽  
Elena Bravo ◽  
Mauro Piacentini ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Gel Electrophoresis ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Maldi Tof

Diversity of Glycoprotein Deficiencies in Glanzmann’s Thrombasthenia

1985 ◽  
Vol 54 (03) ◽  
pp. 626-629 ◽  
Author(s):  
M Meyer ◽  
F H Herrmann
Keyword(s):  
High Resolution ◽  
Gel Electrophoresis ◽  
Type Ii ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Structural Variant ◽  
Glanzmann’S Thrombasthenia ◽  
Crossed Immunoelectrophoresis ◽  
Glanzmann's Thrombasthenia ◽  
Platelet Proteins

SummaryThe platelet proteins of 9 thrombasthenic patients from 7 families were analysed by high resolution two-dimensional gel electrophoresis (HR-2DE) and crossed immunoelectrophoresis (CIE). In 7 patients both glycoproteins (GPs) IIb and Ilia were absent or reduced to roughly the same extent. In two related patients only a trace of GP Ilb-IIIa complex was detected in CIE, but HR-2DE revealed a glycopeptide in the position of GP Ilia in an amount comparable to type II thrombasthenia. This GP Ilia-like component was neither recognized normally by anti-GP Ilb-IIIa antibodies nor labeled by surface iodination. In unreduced-reduced two-dimensional gel electrophoresis two components were observed in the region of GP Ilia. The assumption of a structural variant of GP Ilia in the two related patients is discussed.

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