scholarly journals Targeted Sub-Attomole Cancer Biomarker Detection Based on Phase Singularity 2D Nanomaterial-Enhanced Plasmonic Biosensor

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Yuye Wang ◽  
Shuwen Zeng ◽  
Aurelian Crunteanu ◽  
Zhenming Xie ◽  
Georges Humbert ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Detection Limit ◽  
Biomedical Application ◽  
Lateral Position ◽  
Clinical Diagnostics ◽  
Low Molecular Weight ◽  
List Type ◽  
Two Dimensional ◽  
Phase Singularity ◽  
Position Shift

Highlights A zero-reflection-induced phase singularity is achieved through precisely controlling the resonance characteristics using two-dimensional nanomaterials. An atomically thin nano-layer having a high absorption coefficient is exploited to enhance the zero-reflection dip, which has led to the subsequent phase singularity and thus a giant lateral position shift. We have improved the detection limit of low molecular weight molecules by more than three orders of magnitude compared to current state-of-art nanomaterial-enhanced plasmonic sensors. Abstract Detection of small cancer biomarkers with low molecular weight and a low concentration range has always been challenging yet urgent in many clinical applications such as diagnosing early-stage cancer, monitoring treatment and detecting relapse. Here, a highly enhanced plasmonic biosensor that can overcome this challenge is developed using atomically thin two-dimensional phase change nanomaterial. By precisely engineering the configuration with atomically thin materials, the phase singularity has been successfully achieved with a significantly enhanced lateral position shift effect. Based on our knowledge, it is the first experimental demonstration of a lateral position signal change > 340 μm at a sensing interface from all optical techniques. With this enhanced plasmonic effect, the detection limit has been experimentally demonstrated to be 10–15 mol L−1 for TNF-α cancer marker, which has been found in various human diseases including inflammatory diseases and different kinds of cancer. The as-reported novel integration of atomically thin Ge2Sb2Te5 with plasmonic substrate, which results in a phase singularity and thus a giant lateral position shift, enables the detection of cancer markers with low molecular weight at femtomolar level. These results will definitely hold promising potential in biomedical application and clinical diagnostics.

METHODS FOR REDUCING THE DETECTION LIMIT OF IMMUNOCHROMATOGRAPHIC TEST SYSTEMS FOR ANTIBIOTIC CONTROL IN HONEY

2021 ◽  
Vol 1 (19) ◽  
pp. 219-220
Author(s):  
L. Barshevskaya ◽  
D. Sotnikov ◽  
A. Zherdev ◽  
B. Dzantiev
Keyword(s):  
Molecular Weight ◽  
Detection Limit ◽  
Low Molecular Weight ◽  
Immunochromatographic Test ◽  
Test Systems ◽  
Antibiotic Control

Methods for increasing the sensitivity of immunochromatographic test systems for the determination of low molecular weight analytes are proposed. The developed analysis schemes were tested in the determination of sulfonylamide and chloramphenicol in honey.

Systematic analysis of enoxaparins from different sources with online one- and two-dimensional chromatography

The Analyst ◽  
2019 ◽  
Vol 144 (12) ◽  
pp. 3746-3755
Author(s):  
Yilan Ouyang ◽  
Meng Zhu ◽  
Xin Wang ◽  
Lin Yi ◽  
Jawed Fareed ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Two Dimensional ◽  
Low Molecular Weight Heparins ◽  
Systematic Analysis ◽  
Different Sources

Enoxaparin, one of the most important low-molecular-weight heparins (LMWHs), is widely used as a clinical anticoagulant.

Detection of low-molecular weight allergens resolved on two-dimensional electrophoresis with acid–urea polyacrylamide gel

2006 ◽  
Vol 351 (2) ◽  
pp. 290-297 ◽  
Author(s):  
Kazumi Kitta ◽  
Mayumi Ohnishi-Kameyama ◽  
Tatsuya Moriyama ◽  
Tadashi Ogawa ◽  
Shinichi Kawamoto
Keyword(s):  
Molecular Weight ◽  
Polyacrylamide Gel ◽  
Low Molecular Weight ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

scholarly journals The microsporidian spore invasion tube. The ultrastructure, isolation, and characterization of the protein comprising the tube.

1976 ◽  
Vol 71 (1) ◽  
pp. 23-34 ◽  
Author(s):  
E Weidner
Keyword(s):  
Molecular Weight ◽  
Sodium Dodecyl ◽  
Low Molecular Weight ◽  
Two Dimensional ◽  
Polar Tube ◽  
Isolation And Characterization ◽  
Disulfide Linkages ◽  
Microsporidian Spore ◽  
Ph Range

The extrusion apparatus of the microsporidian parasitic protozoan Nosema michaelis discharges an invasion (or polar) tube with a velocity suitalbe for piercing cells and injecting infective sporoplasm. The tube is composed of a polar tube protein (PTP) which consists of a single, low molecular weight polypeptide slightly smaller than chymotrypsinogen-A. Assembled PTP tubes resist dissociation in sodium dodecyl sulfate and brief exposures in media at extreme ends of the pH range; however, the tubes are reduced by mercaptoethanol and dithiothreitol. When acidified, mercaptoethanol-reduced PTP self-assembles into plastic, two-dimensional monolayers. Dithiothreitol-reduced PTP will not reassemble when acidified. Evidence is presented which indicates that PTP is assembled as a tube within the spore; that the ejected tube has plasticity during sporoplasm passage; and, finally, that the subunits within the tube polymer are bound together, in part, by interprotein disulfide linkages.

Analysis of low molecular weight plasma proteins using ultrafiltration and large gel two-dimensional electrophoresis

PROTEOMICS ◽  
2009 ◽  
Vol 9 (7) ◽  
pp. 1827-1840 ◽  
Author(s):  
Woon-Won Jung ◽  
Sohee Phark ◽  
Sangnam Oh ◽  
Jin-Young Khim ◽  
Juneyoung Lee ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Plasma Proteins ◽  
Low Molecular Weight ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Comprehensive two-dimensional GC for the analysis of low-molecular-weight oxygenates in three different matrices from a petrochemical pilot plant using a single calibration

2014 ◽  
Vol 37 (5) ◽  
pp. 566-572 ◽  
Author(s):  
Wonga Hexana ◽  
Piet De Coning ◽  
Samuel Jali ◽  
Rina Van der Westhuizen ◽  
Bryan Brack ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Pilot Plant ◽  
Low Molecular Weight ◽  
Two Dimensional ◽  
Single Calibration

The RNA Fractions of Chromatin from L-Cells

1974 ◽  
Vol 52 (10) ◽  
pp. 922-935 ◽  
Author(s):  
John J. Monahan ◽  
Ross H. Hall
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Weight Fraction ◽  
Polyacrylamide Gel ◽  
Low Molecular Weight ◽  
Two Dimensional ◽  
Cell Nuclei ◽  
L Cell

Chromatin obtained from L-cell nuclei has been shown to contain three distinct RNA fractions. One, a low molecular weight RNA fraction (3–7 S), has been isolated and purified. Two-dimensional polyacrylamide gel electrophoresis indicates that this fraction contains at least 28 diverse RNA components. Both the rate of incorporation of [32P]H3PO4 into this fraction and the loss of [3H]uridine from the fraction after a 1 h pulse suggest that these RNA's are slowly synthesized and are very stable. Not all the species within the low molecular weight fraction have the same turnover rates.A high molecular weight RNA fraction (15–30 S) has also been isolated from L-cell chromatin. From the rate of [32P]H3PO4 incorporation and the loss of [3H]uridine from this fraction after a 1 h pulse, it appears that this RNA fraction is rapidly synthesized and is either unstable or is rapidly removed from chromatin once synthesized.A third RNA fraction also present in L-cell chromatin appears to be tightly bound to the DNA. It has been isolated and shown to be of low molecular weight (3–5 S). This RNA also appears to be rapidly synthesized and to be metabolically unstable.

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