Development of a PCR protocol for the identification and detection of Fusarium solani f. sp. piperis from soil and roots of black pepper (Piper nigrum)

2017 ◽  
Vol 42 (1) ◽  
pp. 55-59 ◽  
Author(s):  
Sarah S. Costa ◽  
Gláucia M. Moreira ◽  
Ludwig H. Pfenning
Keyword(s):  
Fusarium Solani ◽  
Black Pepper ◽  
Piper Nigrum

scholarly journals In vitro toxin production by Fusarium solani f. sp. piperis

2003 ◽  
Vol 28 (3) ◽  
pp. 229-235 ◽  
Author(s):  
Maria de Lourdes R. Duarte ◽  
Simon A. Archer
Keyword(s):  
Fusarium Solani ◽  
Biological Response ◽  
Black Pepper ◽  
Biologically Active ◽  
Piper Nigrum ◽  
Piper Betle ◽  
Toxic Metabolites ◽  
Culture Filtrates ◽  
Detached Leaves

Fusarium solani f. sp. piperis (teleomorph: Nectria haematococca f. sp. piperis), causal agent of root rot and stem blight on black pepper (Piper nigrum), produces secondary metabolites with toxigenic properties, capable of inducing vein discoloration in detached leaves and wilting in transpiring microcuttings. Production of F. solani f. sp. piperis (Fsp) toxic metabolites reached a peak after 25 days of static incubation on potato sucrose broth at 25 ºC under illumination. Changes in the pH of the culture filtrate did not alter the effect of toxic metabolites. However, when the pH was changed before the medium had been autoclaved, a more intense biological response was observed, with an optimum at pH 6.0. Isolates that produced red pigments in liquid cultures were more efficient in producing biologically active culture filtrates than those which produced pink coloured or clear filtrates suggesting that these pigments could be related to toxigenic activity. Detached leaves of seven black pepper cultivars and Piper betle showed symptoms of vein discoloration after immersion in autoclaved and non-autoclaved Fsp culture filtrates indicating the thermostable nature of these toxic metabolites.

scholarly journals COMPORTAMENTO ESPACIAL DA FUSARIOSE E DOS ATRIBUTOS DO SOLO NO CULTIVO DA PIMENTA-DO-REINO

Nativa ◽  
2018 ◽  
Vol 6 (2) ◽  
pp. 113
Author(s):  
Carla Da Penha Simon ◽  
Ana Carolina Lyra Brumat ◽  
Marcelo Barreto Da Silva ◽  
Bernardo Torres Olmo ◽  
Edney Leandro da Vitória ◽  
...  
Keyword(s):  
Organic Matter ◽  
Soil Texture ◽  
Fusarium Solani ◽  
Nutrient Content ◽  
Black Pepper ◽  
Irregular Sampling ◽  
Piper Nigrum ◽  
Soil Attributes ◽  
Calcium Magnesium ◽  
Chemical Attributes

A pimenta-do-reino é a especiaria mais consumida no mundo e o Brasil destaca-se como um dos maiores produtores. Um dos grandes limitantes no seu cultivo é a fusariose (Fusarium solani f. sp. piperis). Objetivou-se com a realização deste estudo caracterizar a variabilidade espacial da fusariose em pimenta-do-reino, verificando a existência de relação com os atributos físicos e químicos do solo. O estudo foi desenvolvido em uma lavoura localizada no município de São Mateus-ES, na qual foi estabelecida uma malha amostral irregular com 79 pontos, abrangendo uma área de um hectare.  Para a amostragem da fusariose foi realizado um levantamento, onde uma planta por ponto da malha amostral foi classificada em sadia, doente ou morta.  Os atributos do solo amostrados foram: pH em H2O, matéria orgânica, cálcio, magnésio, potássio e textura do solo. Os dados foram analisados através da estatística descritiva e ferramentas da geoestatística. Os semivariogramas ajustados apresentaram uma forte dependência espacial para as variáveis intensidade da fusariose, altimetria, matéria orgânica, textura do solo, pH, cálcio, magnésio potássio (89, 94, 92, 94, 93, 91, 100 e 85 % respectivamente). Os mapas gerados indicam que não há relação da intensidade da fusariose com os atributos do solo estudados no experimento.Palavra-chave: epidemiologia, Fusarium solani f. sp. piperis, geoestatística, Piper nigrum L. SPATIAL ANALYSIS OF FUSARIOSE AND SOIL ATTRIBUTES IN THE BLACK PEPPER CULTIVATION  ABSTRACT:Black pepper is the most consumed spice in the world, Brazil stands out as one of the largest producers. One of the major constraints in cultivation is fusariosis (Fusarium solani f. Piperis). The objective of this study was characterize the spatial variability of fusariosis in black pepper and verify the relationship of chemical attributes of the soil. The study was developed in a tillage located in the municipality of São Mateus - ES, which exist an irregular sampling network was established with 79 points, covering an area of one hectare. For the sampling of fusariosis intensity, the survey was performed, where one plant per point of the sample mesh was classified as healthy, symptomatic or dead. The attributes of the soil sampled were: pH in H2O, organic matter, nutrient content (calcium, magnesium, potassium) and soil texture. Data were analyzed through descriptive statistics and geostatistics tools. The adjusted semivariograms indicated a strong spatial dependence for the variables intensity of fusariosis, altimetry, organic matter, soil texture, pH, calcium, potassium magnesium (89, 94, 92, 94, 93, 91, 100 and 85%, respectively). The generated maps indicate that there is no relation between the incidence of fusariosis and the soil attributes studied in the experiment.Keywords: epidemiology, Fusarium solani f. sp. piperis, geostatistics, Piper nigrum L. DOI:

scholarly journals Secondary Metabolic Profiles of Two Cultivars of Piper nigrum (Black Pepper) Resulting from Infection by Fusarium solani f. sp. piperis

2017 ◽  
Vol 18 (12) ◽  
pp. 2434 ◽  
Author(s):  
Shirlley da Luz ◽  
Lydia Yamaguchi ◽  
Massuo Kato ◽  
Oriel de Lemos ◽  
Luciana Xavier ◽  
...  
Keyword(s):  
Fusarium Solani ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Metabolic Profiles

scholarly journals COMPORTAMENTO ESPAÇO-TEMPORAL DA FUSARIOSE EM PIMENTA-DO-REINO

Nativa ◽  
2019 ◽  
Vol 7 (5) ◽  
pp. 484
Author(s):  
Ana Carolina Lyra Brumat ◽  
Marcelo Silva ◽  
Antonio Fernando de Souza ◽  
Ivoney Gontijo ◽  
Edney Leandro da Vitória ◽  
...  
Keyword(s):  
Fusarium Solani ◽  
Temporal Dynamics ◽  
Temporal Distribution ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Progress Curve ◽  
Taylor’S Law ◽  
Spatio Temporal ◽  
The One ◽  
Best Fit

Objetivou-se com o trabalho estudar a dinâmica de distribuição espaço-temporal da fusariose em pimenta-do-reino. Foram monitoradas quatro áreas de plantio, no município de São Mateus-ES, no período de dez/2015 a dez/2016. Realizaram-se seis avaliações da doença. As plantas foram avaliadas visualmente em busca dos sintomas típicos da doença. A presença ou ausência de sintomas, e a posição de cada planta, foram anotadas em planilhas obtendo-se o mapeamento da evolução da doença dentro das áreas. Com base nos mapas criados fez-se o estudo da distribuição espaço-temporal. A partir dos dados de incidência calculou-se a área abaixo da curva de progresso da doença (AACPD) e realizou-se o ajuste de modelos epidemiológicos.  Para o estudo espacial utilizou-se a Lei de Taylor modificada, o Índice de Dispersão (ID) e a análise de dinâmica e estrutura de focos (ADEF). O modelo monomolecular foi o que melhor se ajustou à curva de progresso da fusariose.  Observou-se que os resultados de ID variaram em cada área. A Lei de Taylor modificada indicou distribuição agregada somente em uma área.  A ADEF demonstrou que com a evolução da doença ao longo do tempo os focos se tornavam maiores e em menor número.Palavras-chave: Piper nigrum; Fusarium solani f. sp. piperis; epidemiologia. SPATIO-TEMPORAL BEHAVIOR OF FUSARIOSIS IN BLACK PEPPER ABSTRACT: The objective of this study was to study the spatio-temporal dynamics of fusariosis in black pepper. Four planting areas were monitored in the municipality of São Mateus-ES, in the period from 10/2015 to 10 / 2016. Six classifieds of the disease were carried out. The plants were visually evaluated for the typical symptoms of the disease. The presence or absence of symptoms, and the position of each plant, were recorded on planets and the mapping of the evolution of the disease within the areas was obtained. Based on the sites visited, he studied the spatial-temporal distribution. From the calculation data, an area under the disease progress curve (AACPD) is calculated and adjusted for epidemiological models. For the spatial study we used a modified Taylor's Law, the Dispersion Index (ID) and a dynamics and foci structure analysis (ADEF). The monomolecular model was the one that best fit the progression curve of fusariosis. It was observed that the ID results varied in each area. The modified Taylor Law indicated the distribution of content in an area. ADEF demonstrated an evolution of the disease over time as the outbreaks became larger and smaller.Keywords: Piper nigrum; Fusarium solani f. sp. piperis; epidemiology.

scholarly journals Transcriptional profiling by RNA sequencing of black pepper (Piper nigrum L.) roots infected by Fusarium solani f. sp. piperis

2017 ◽  
Vol 39 (10) ◽  
Author(s):  
Edith C. O. Moreira ◽  
Daniel G. Pinheiro ◽  
Sheila M. C. Gordo ◽  
Simone M. Rodrigues ◽  
Elaine Pessoa ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Fusarium Solani ◽  
Transcriptional Profiling ◽  
Black Pepper ◽  
Piper Nigrum

scholarly journals First Report of Sexual Reproduction of Fusarium solani f. sp. piperis in Bahia, Brazil

Plant Disease ◽  
2012 ◽  
Vol 96 (10) ◽  
pp. 1581-1581 ◽  
Author(s):  
A. B. Vaz ◽  
V. G. Elizei ◽  
S. S. Costa ◽  
L. H. Pfenning ◽  
J. A. Ventura
Keyword(s):  
Fusarium Solani ◽  
Early Death ◽  
Distinct Species ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Stem Cuttings ◽  
First Report ◽  
Sterile Culture ◽  
Leaf Chlorosis ◽  
Initial Symptoms

Black pepper (Piper nigrum L.) is a popular spice native of India, and Brazil is one of its most important producing countries. The main disease of black pepper in Brazil is fusariosis, caused by Fusarium solani f. sp. piperis. Symptoms include leaf chlorosis and defoliation, blight of stems or stem cuttings, and root and foot decay. During surveys conducted in the south of the state of Bahia, municipalities of Taperoá (13°34'S, 39°10'W) and Valencia (13°20'S, 39°14'W), stems of diseased plants covered with red or salmon-colored perithecia were observed, while twigs showed leaf chlorosis, leading to early death of the plants. Ascomata were solitary or in groups, mostly superficial or surrounded by mycelia, globose, subglobose, ovoid, and 122 to 400 μm diameter. Microscopic examination revealed unitunicate, cylindric asci, 60 to 90 × 8.5 to 16 μm, thin-walled, containing eight ascospores arranged obliquely in two rows. Ascospores are hyaline, elliptical to oblong, one-septate, constricted at the central septum, 10 to 16 × 4 to 6.5 μm (means ± S.D.: 13.1 ± 1.4 × 5.1 ± 0.6 μm), length/width (L/W) 1.9 to 3.7. Single-spored cultures were transferred to SNA medium (incubated at 20°C for 7 days with 12-h photoperiod) and on potato dextrose agar (25°C in dark) for characterization. The anamorph is characterized by the presence of chlamydospores, canoe-shaped sporodochial macroconidia with three to four septae, and microconidia formed on long monophialidic conidiophores. Based on morphological markers, isolates were identified as F. solani. The partial fragment of the TEF-1α gene of single-spored isolates (CML 2186, 2187, 2188, 2189, 2190, and 2191) were sequenced. BLAST analysis of the sequence resulted in 94 to 99% identity with a reference strain of F. solani f. sp. piperis (NRRL 22570, CML 1888). For pathogenicity tests, cv. Bragantina was used and two isolates were inoculated as 5-mm diameter mycelial plugs on the stem of four plants each. Four control plants were treated only with sterile culture medium. Plants were maintained in the greenhouse at 25°C and 75 to 85% relative humidity under 70% shade. All inoculated plants showed initial symptoms of stem necrosis in inoculated branches 7 days after inoculation. Symptoms were not observed on stems of control plants. Isolates were successfully reisolated and identified as F. solani f. sp. piperis, fulfilling Koch's postulates. Representative isolates were deposited at the Coleção Micológica de Lavras (CML) at Universidade Federal de Lavras, Brazil. Production of perithecia of the pathogen has been previously reported only in Pará and Espírito Santo States (1,3). It is not yet confirmed if this taxon is homothallic or heterothallic. To our knowledge, this is the first report of the associated teleomorph of F. solani f. sp. piperis infecting and causing black pepper fusariosis in Bahia, Brazil. The results suggest that the spread of ascospores from perithecia is likely to be one of the main inoculum sources of the disease on adjacent vines. There is evidence that this special form of F. solani actually represents a distinct species pathogenic to black pepper (2). References: (1) F. C. Albuquerque and S. Ferraz. Experientiae 22:133, 1976. (2) K. O'Donnell. Mycologia 92:919, 2000. (3) J. A. Ventura et al. Fitopatol. Bras. 11:361, 1986.

Antileishmanial Alkaloids from Black Pepper (Piper nigrum)

Planta Medica ◽  
2011 ◽  
Vol 77 (05) ◽  
Author(s):  
HRW Dharmaratne ◽  
BL Tekwani ◽  
NPD Nanayakkara
Keyword(s):  
Black Pepper ◽  
Piper Nigrum

DETERMINATION OF THE EFFICIENCY AND EFFICACY OF BIO-CHEMICAL FERTILIZER ON THE SOIL, YIELD AND GROWTH PERFORMANCE OF BLACK PEPPER (Piper nigrum L.)

2015 ◽  
Vol 4 (3) ◽  
pp. 460-468
Author(s):  
Yap Chin Ann
Keyword(s):  
Growth Performance ◽  
Nutrient Management ◽  
High Growth ◽  
Black Pepper ◽  
Chemical Fertilizer ◽  
Piper Nigrum ◽  
High Fertility ◽  
Fertility Level ◽  
Area Index ◽  
K 12

The last nutrient management review of black pepper was done in 1968. There is, therefore, a need to develop new technology to improve pepper production and transfer that technology to production site. This experiment was carried out to study the effect of newly developed biochemical fertilizer on some physiological characteristics, yield and soil fertility of pepper. The treatment consisted of T1 (BS): chemical fertilizer (N:12%, P:12%, K:17%); T2 (BK1): biochemical fertilizer F1 N:15%, P:5%, K:14) and T3 (BK2): biochemical fertilizer F2 (N:13%, P:4%, K:12). The biochemical fertilizer F1 out-yielded chemical and biochemical fertilizer F2 by 75.38% and 16.45% respectively with the higher yield being associated with various phonotypical alterations, which are reported here. Significant measureable changes were observed in physiological processes and plant characteristics, such as large leaf area index, more chlorophyll content and high photosynthesis rate coupled with lower transpiration rate in biochemical fertilizer F1(BK1) treatment compared with other treatment. The high fertility level in biochemical fertilizer F1 and biochemical fertilizer F2 (BK2) reflected the important of organic material in improving soil quality. In conclusion, the achieve high growth performance and yield in pepper, chemical fertilizer alone is insufficient whilst combination of organic and inorganic fertilizer with balance nutrient content gave a significant increase in yield and growth of pepper. 

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