scholarly journals In vitro toxin production by Fusarium solani f. sp. piperis

2003 ◽  
Vol 28 (3) ◽  
pp. 229-235 ◽  
Author(s):  
Maria de Lourdes R. Duarte ◽  
Simon A. Archer
Keyword(s):  
Fusarium Solani ◽  
Biological Response ◽  
Black Pepper ◽  
Biologically Active ◽  
Piper Nigrum ◽  
Piper Betle ◽  
Toxic Metabolites ◽  
Culture Filtrates ◽  
Detached Leaves

Fusarium solani f. sp. piperis (teleomorph: Nectria haematococca f. sp. piperis), causal agent of root rot and stem blight on black pepper (Piper nigrum), produces secondary metabolites with toxigenic properties, capable of inducing vein discoloration in detached leaves and wilting in transpiring microcuttings. Production of F. solani f. sp. piperis (Fsp) toxic metabolites reached a peak after 25 days of static incubation on potato sucrose broth at 25 ºC under illumination. Changes in the pH of the culture filtrate did not alter the effect of toxic metabolites. However, when the pH was changed before the medium had been autoclaved, a more intense biological response was observed, with an optimum at pH 6.0. Isolates that produced red pigments in liquid cultures were more efficient in producing biologically active culture filtrates than those which produced pink coloured or clear filtrates suggesting that these pigments could be related to toxigenic activity. Detached leaves of seven black pepper cultivars and Piper betle showed symptoms of vein discoloration after immersion in autoclaved and non-autoclaved Fsp culture filtrates indicating the thermostable nature of these toxic metabolites.

scholarly journals COMPORTAMENTO ESPACIAL DA FUSARIOSE E DOS ATRIBUTOS DO SOLO NO CULTIVO DA PIMENTA-DO-REINO

Nativa ◽  
2018 ◽  
Vol 6 (2) ◽  
pp. 113
Author(s):  
Carla Da Penha Simon ◽  
Ana Carolina Lyra Brumat ◽  
Marcelo Barreto Da Silva ◽  
Bernardo Torres Olmo ◽  
Edney Leandro da Vitória ◽  
...  
Keyword(s):  
Organic Matter ◽  
Soil Texture ◽  
Fusarium Solani ◽  
Nutrient Content ◽  
Black Pepper ◽  
Irregular Sampling ◽  
Piper Nigrum ◽  
Soil Attributes ◽  
Calcium Magnesium ◽  
Chemical Attributes

A pimenta-do-reino é a especiaria mais consumida no mundo e o Brasil destaca-se como um dos maiores produtores. Um dos grandes limitantes no seu cultivo é a fusariose (Fusarium solani f. sp. piperis). Objetivou-se com a realização deste estudo caracterizar a variabilidade espacial da fusariose em pimenta-do-reino, verificando a existência de relação com os atributos físicos e químicos do solo. O estudo foi desenvolvido em uma lavoura localizada no município de São Mateus-ES, na qual foi estabelecida uma malha amostral irregular com 79 pontos, abrangendo uma área de um hectare.  Para a amostragem da fusariose foi realizado um levantamento, onde uma planta por ponto da malha amostral foi classificada em sadia, doente ou morta.  Os atributos do solo amostrados foram: pH em H2O, matéria orgânica, cálcio, magnésio, potássio e textura do solo. Os dados foram analisados através da estatística descritiva e ferramentas da geoestatística. Os semivariogramas ajustados apresentaram uma forte dependência espacial para as variáveis intensidade da fusariose, altimetria, matéria orgânica, textura do solo, pH, cálcio, magnésio potássio (89, 94, 92, 94, 93, 91, 100 e 85 % respectivamente). Os mapas gerados indicam que não há relação da intensidade da fusariose com os atributos do solo estudados no experimento.Palavra-chave: epidemiologia, Fusarium solani f. sp. piperis, geoestatística, Piper nigrum L. SPATIAL ANALYSIS OF FUSARIOSE AND SOIL ATTRIBUTES IN THE BLACK PEPPER CULTIVATION  ABSTRACT:Black pepper is the most consumed spice in the world, Brazil stands out as one of the largest producers. One of the major constraints in cultivation is fusariosis (Fusarium solani f. Piperis). The objective of this study was characterize the spatial variability of fusariosis in black pepper and verify the relationship of chemical attributes of the soil. The study was developed in a tillage located in the municipality of São Mateus - ES, which exist an irregular sampling network was established with 79 points, covering an area of one hectare. For the sampling of fusariosis intensity, the survey was performed, where one plant per point of the sample mesh was classified as healthy, symptomatic or dead. The attributes of the soil sampled were: pH in H2O, organic matter, nutrient content (calcium, magnesium, potassium) and soil texture. Data were analyzed through descriptive statistics and geostatistics tools. The adjusted semivariograms indicated a strong spatial dependence for the variables intensity of fusariosis, altimetry, organic matter, soil texture, pH, calcium, potassium magnesium (89, 94, 92, 94, 93, 91, 100 and 85%, respectively). The generated maps indicate that there is no relation between the incidence of fusariosis and the soil attributes studied in the experiment.Keywords: epidemiology, Fusarium solani f. sp. piperis, geostatistics, Piper nigrum L. DOI:

scholarly journals Comparative Analysis of Intracellular and in vitro Antioxidant Activities of Essential Oil From White and Black Pepper (Piper nigrum L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Ying Wang ◽  
Liang Wang ◽  
Jin Tan ◽  
Rong Li ◽  
Zi-Tao Jiang ◽  
...  
Keyword(s):  
Essential Oils ◽  
Antioxidant Activities ◽  
Cell Model ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Anti Cancer ◽  
Total Antioxidant ◽  
Therapeutic Tools ◽  
Median Effective Concentration

Ethnopharmacological Relevance: Pepper essential oils have potential immunomodulatory, anti-tumor, and anti-cancer activities. Pepper exhibits the potential to prevent or attenuate carcinogenesis as therapeutic tools. However, the related mechanism remains unelucidated.Aim of the Study: The present study aims to provide reasonable information for the explanation of the dissimilarity of the essential oils from white (WPEO) and black pepper (BPEO).Materials and Methods: WPEO, BPEO, and their single active component, as well as synthetic antioxidants, were compared by the cell model methods and chemical methods, including intracellular antioxidant activity (CAA), total antioxidant activities (TAA), superoxide radical (SR), hydroxyl radical (HR), DPPH radical (DR) scavenging activities and inhibition ability of lipoprotein lipid peroxidation (ILLP).Results: The median effective concentration (EC50) values (mg/mL) of the WPEO and BPEO of SR, HR, DR, and ILLP were 0.437 and 0.327, 0.486 and 0.204, 7.332 and 6.348, 0.688, and 0.624 mg/mL, respectively. The CAA units of WPEO and BPEO were 50.644 and 54.806, respectively. CAA, DR, and TAA of BPEO were significantly higher than those of WPEO (p < 0.05). The BPEO and WPEO can be differentiated as the former have higher correlations with 3-carene, α-pinene, β-pinene, and limonene while the latter has a higher caryophyllene correlation. The WPEO and BPEO show a good intracellular scavenging ability of reactive oxygen species in HeLa cells.Conclusion: Generally, pepper oil has stronger activities than single components, indicating that pepper is a broad-spectrum natural antioxidant.

EXTRACTION, ISOLATION, CHEMICAL IDENTIFICATION AND IN VITRO UROLITHIATIC ACTIVITY OF PIPERINE FROM PIPER NIGRUM

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (11) ◽  
pp. 38-41
Author(s):  
Jyoti ◽  
Yogesh Murti ◽  
Krishn K. Agrawal ◽  
Keyword(s):  
Calcium Oxalate ◽  
Black Pepper ◽  
Semipermeable Membrane ◽  
Piper Nigrum ◽  
Chemical Identification ◽  
Calcium Oxalate Crystals ◽  
Chemical Reagents ◽  
Oxalate Crystals ◽  
The Family

Piper nigrum is belongs to the family Piperaceae. The aim of the present study was to extract, isolate and investigate in vitro urolithiatic activity of alkaloid (piperine) from black pepper and identification by chemical reagents. In vitro urolithiatic evaluation of piperine was performed by percentage dissolution and turbidity methods. Calcium oxalate crystals were synthetically prepared and packed in a semipermeable membrane in both methods. Results were compared statistically and it showed significant P​

scholarly journals Secondary Metabolic Profiles of Two Cultivars of Piper nigrum (Black Pepper) Resulting from Infection by Fusarium solani f. sp. piperis

2017 ◽  
Vol 18 (12) ◽  
pp. 2434 ◽  
Author(s):  
Shirlley da Luz ◽  
Lydia Yamaguchi ◽  
Massuo Kato ◽  
Oriel de Lemos ◽  
Luciana Xavier ◽  
...  
Keyword(s):  
Fusarium Solani ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Metabolic Profiles

scholarly journals Prebiotic Potential of Culinary Spices Used to Support Digestion and Bioabsorption

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Christine T. Peterson ◽  
Dmitry A. Rodionov ◽  
Stanislav N. Iablokov ◽  
Meredith A. Pung ◽  
Deepak Chopra ◽  
...  
Keyword(s):  
Human Subjects ◽  
Curcuma Longa ◽  
Glycosyl Hydrolase ◽  
Health Benefits ◽  
Zingiber Officinale ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Sugar Composition ◽  
The Impact

Although the impact of medicinal and culinary herbs on health and disease has been studied to varying extents, scarcely little is known about the impact of these herbs on gut microbiota and how such effects might contribute to their health benefits. We applied in vitro anaerobic cultivation of human fecal microbiota followed by 16S rRNA sequencing to study the modulatory effects of 4 culinary spices: Curcuma longa (turmeric), Zingiber officinale (ginger), Piper longum (pipli or long pepper), and Piper nigrum (black pepper). All herbs analyzed possessed substantial power to modulate fecal bacterial communities to include potential prebiotic and beneficial repressive effects. We additionally analyzed the sugar composition of each herb by mass spectrometry and conducted genome reconstruction of 11 relevant sugar utilization pathways, glycosyl hydrolase gene representation, and both butyrate and propionate biosynthesis potential to facilitate our ability to functionally interpret microbiota profiles. Results indicated that sugar composition is not predictive of the taxa responding to each herb; however, glycosyl hydrolase gene representation is strongly modulated by each herb, suggesting that polysaccharide substrates present in herbs provide selective potential on gut communities. Additionally, we conclude that catabolism of herbs by gut communities primarily involves sugar fermentation at the expense of amino acid metabolism. Among the herbs analyzed, only turmeric induced changes in community composition that are predicted to increase butyrate-producing taxa. Our data suggests that substrates present in culinary spices may drive beneficial alterations in gut communities thereby altering their collective metabolism to contribute to the salubrious effects on digestive efficiency and health. These results support the potential value of further investigations in human subjects to delineate whether the metabolism of these herbs contributes to documented and yet to be discovered health benefits.

scholarly journals Awareness of Medicinal Applications of Piper nigrum among Dental Students

2020 ◽  
Vol 11 (SPL3) ◽  
pp. 927-930
Author(s):  
Nithyanandham Masilamani ◽  
Dhanraj Ganapathy
Keyword(s):  
Side Effects ◽  
Mechanism Of Action ◽  
Black Pepper ◽  
Dental Students ◽  
Biologically Active ◽  
Piper Nigrum ◽  
Medical Applications ◽  
Therapeutic Effects ◽  
White Pepper ◽  
Anti Inflammatory

Black pepper includes the many commonly used ingredients upon this planet, remarkable for some of its potent component piperine. White pepper is made with closely related species, while black pepper is prepared by fast processing and wind drying of underripe natural products, white pepper is made from dried, regular and ready-mixed seeds. Piperine has been the conventional biologically active conjugate of Piper nigrum and Piper longum, having been taken into consideration for therapeutic effects. The purpose of this survey was for assessing the awareness of medicinal applications of Piper nigrum amongst dental students. A cross-sectional survey was done with a self-administered questionnaire with 10 questions circulated among 100 dental students. The questionnaire assessed the awareness about Piper nigrumtherapy in medical applications, their immunomodulatory properties, antipyretic properties, antispasmodic activity, anti-inflammatory activity, and its mechanism of action and side effects. The responses were recorded and analysed. 16% of the respondents were aware of the medical applications of Piper nigrum therapy.11 % were aware of the anti immunomodulatory activity of Piper nigrum therapy .9 % were aware of antipyretic properties of Piper nigrum therapy .13 % were aware of antispasmodic properties of Piper nigrum therapy .10 % were aware of anti-inflammatory properties of Piper nigrum therapy . 6 % were an aware mechanism of action and side effects of Piper nigrum therapy. The awareness about the use of Piper nigrum therapy in medicinal applications is low among dental students. Increased awareness programs and sensitization and continuing dental education programs along with greater importance to the curricular modifications, can further enhance knowledge and awareness about Piper nigrum therapy.

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