Aggressiveness and genetic diversity of Ralstonia solanacearum strains from tomato in Vietnam

2018 ◽  
Vol 71 (4) ◽  
pp. 599-610
Author(s):  
Hai Thi Hong Truong ◽  
Thanh-Thuy Duong ◽  
Tram Thi Hoai Nguyen ◽  
Thuy Thi Thu Nguyen ◽  
Linh Hoang Khanh Nguyen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ralstonia Solanacearum

Genetic Diversity of Ralstonia solanacearum Race 3 in Western Europe determined by AFLP, RC-PFGE and Rep-PCR

1998 ◽  
pp. 44-49 ◽  
Author(s):  
J. M. Van Der Wolf ◽  
P. J. M. Bonants ◽  
J. J. Smith ◽  
M. Hagenaar ◽  
E. Nijhuis ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ralstonia Solanacearum ◽  
Western Europe

scholarly journals Broad Diversity of Ralstonia solanacearum Strains in Cameroon

Plant Disease ◽  
2009 ◽  
Vol 93 (11) ◽  
pp. 1123-1130 ◽  
Author(s):  
Gabriel Mahbou Somo Toukam ◽  
Gilles Cellier ◽  
Emmanuel Wicker ◽  
Caroline Guilbaud ◽  
Rémi Kahane ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ralstonia Solanacearum ◽  
Central Africa ◽  
Sequence Analyses ◽  
Chain Reaction ◽  
Specific Pcr ◽  
Economic Threat ◽  
The Indian Ocean ◽  
Polymerase Chain ◽  
Reference Strains

In 2005, an extensive survey of bacterial wilt in Cameroon collected 110 strains of Ralstonia solanacearum from wilting tomato, potato, pepper, huckleberry (Solanum scabrum), sesame, and amaranth. The genetic diversity and phylogeny of selected strains from Cameroon were assessed by multiplex–polymerase chain reaction (PCR), race 3/biovar 2–specific PCR, and sequence analyses of the mutS and egl genes. These data were compared with those from 33 reference strains covering the known diversity within the R. solanacearum species complex. Strains isolated in Cameroon clustered into three of the four known phylotypes: I (Asian), II (American), and III (African). Lowland tomato strains belonged to phylotype I and were quite homogeneous. The strains belonging to phylotype II were genetically diverse, and partitioned into subclusters IIA and IIB (sequevar 1, race 3/biovar 2). Cameroon strains in the African phylotype III were distinct from reference strains from Zimbabwe or the Indian Ocean, highlighting the genetic diversity present within this phylotype. Strains from potatoes growing in the highlands of West Cameroon fell into both phylotypes II (race 3/biovar 2) and III. These phylotype II and III highland strains attacked both potato and tomato and could therefore pose an economic threat to potato and tomato crops throughout Central Africa. This is the first comprehensive report on the genetic diversity of R. solanacearum strains in Cameroon.

scholarly journals Genetic Diversity and Aggressiveness of Ralstonia solanacearum Strains Causing Bacterial Wilt of Potato in Uruguay

Plant Disease ◽  
2011 ◽  
Vol 95 (10) ◽  
pp. 1292-1301 ◽  
Author(s):  
M. I. Siri ◽  
A. Sanabria ◽  
M. J. Pianzzola
Keyword(s):  
Genetic Diversity ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Potato Tubers ◽  
Bacterial Strains ◽  
Potato Plants ◽  
Polymerase Chain ◽  
First Time ◽  
Different Levels

Bacterial wilt, caused by Ralstonia solanacearum, is a major disease affecting potato (Solanum tuberosum) production worldwide. Although local reports suggest that the disease is widespread in Uruguay, characterization of prevalent R. solanacearum strains in that country has not been done. In all, 28 strains of R. solanacearum isolated from major potato-growing areas in Uruguay were evaluated, including 26 strains isolated from potato tubers and 2 from soil samples. All strains belonged to phylotype IIB, sequevar 1 (race 3, biovar 2). Genetic diversity of strains was assessed by repetitive-sequence polymerase chain reaction, which showed that the Uruguayan strains constituted a homogeneous group. In contrast, inoculation of the strains on tomato and potato plants showed, for the first time, different levels of aggressiveness among R. solanacearum strains belonging to phylotype IIB, sequevar 1. Aggressiveness assays were also performed on accessions of S. commersonii, a wild species native to Uruguay that is a source of resistance for potato breeding. No significant interactions were found between bacterial strains and potato and S. commersonii genotypes, and differences in aggressiveness among R. solanacearum strains were consistent with previously identified groups based on tomato and potato inoculations. Moreover, variation in responses to R. solanacearum was observed among the S. commersonii accessions tested.

scholarly journals Genetic Diversity of the Ralstonia solanacearum Species Complex in the Southwest Indian Ocean Islands

2017 ◽  
Vol 8 ◽  
Author(s):  
Noura Yahiaoui ◽  
Jean-Jacques Chéron ◽  
Santatra Ravelomanantsoa ◽  
Azali A. Hamza ◽  
Bobb Petrousse ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Indian Ocean ◽  
Ralstonia Solanacearum ◽  
Species Complex ◽  
Southwest Indian Ocean ◽  
Ocean Islands

scholarly journals Genetic Diversity of Zingiberaceae Plant Isolates of Ralstonia solanacearum in the Asia-Pacific Region

2013 ◽  
Vol 47 (3) ◽  
pp. 283-294 ◽  
Author(s):  
Takamitsu WAKI ◽  
Mitsuo HORITA ◽  
Daisuke KUROSE ◽  
Karden MULYA ◽  
Kenichi TSUCHIYA
Keyword(s):  
Genetic Diversity ◽  
Ralstonia Solanacearum ◽  
Asia Pacific ◽  
Pacific Region ◽  
Asia Pacific Region

scholarly journals GENETIC DIVERSITY OF BACTERIAL WILT CAUSED BY RALSTONIA SOLANACEARUM AS ASSESSED BY PCR

2017 ◽  
Vol 29 (1) ◽  
pp. 69
Author(s):  
Muhammad Mansoor ◽  
Muhammad W. Abbas ◽  
J. D V. Elsas ◽  
Muhammad R. Bashir ◽  
Muhammad Atiq
Keyword(s):  
Genetic Diversity ◽  
Molecular Biology ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Wilt Disease ◽  
Multi Locus Sequence Typing ◽  
Chain Reaction ◽  
Bacterial Wilt Disease ◽  
Different Strains ◽  
Modern Tool

The current research was conducted to investigate genetic diversity of Ralstonia solanacearum for comparison of different strains that were collected mainly from Netherlands as well as from Bangladesh, Brazil, Kenya, Egypt, Pakistan and Palma. Forty six strains were included in contemporary studies whereas main biovars for these strains included biovar-2 except GMI1000 that belonged to biovar 3. Genetic diversity of bacterial wilt disease caused by R. solancearum was assessed by focusing mainly on three genes i.e. mutL, cbhA and dps. All the genes seem to be conserved but in case of mutL some strains showed divergence. Multi Locus Sequence Typing (MLST) scheme was used in this contemporary research. It was concluded that polymerized chain reaction (PCR) is the most imperative and appropriate modern tool of molecular biology to find genetic diversity in Ralstonia solanacearum causing bacterial wilt.     

Wide genetic diversity of Ralstonia solanacearum strains affecting tomato in Trinidad, West Indies

2012 ◽  
Vol 61 (5) ◽  
pp. 844-857 ◽  
Author(s):  
A. Ramsubhag ◽  
D. Lawrence ◽  
D. Cassie ◽  
R. Fraser ◽  
P. Umaharan ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ralstonia Solanacearum ◽  
West Indies

Genetic diversity amongst Ralstonia solanacearum isolates of potato in Europe

EPPO Bulletin ◽  
1998 ◽  
Vol 28 (1-2) ◽  
pp. 83-84 ◽  
Author(s):  
J. J. SMITH ◽  
J. M. WOLF ◽  
R. FEUILLADE ◽  
A. TRIGALET ◽  
L. C. OFFORD ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ralstonia Solanacearum
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