Genetic Diversity of Ralstonia solanacearum Race 3 in Western Europe determined by AFLP, RC-PFGE and Rep-PCR

1998 ◽  
pp. 44-49 ◽  
Author(s):  
J. M. Van Der Wolf ◽  
P. J. M. Bonants ◽  
J. J. Smith ◽  
M. Hagenaar ◽  
E. Nijhuis ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ralstonia Solanacearum ◽  
Western Europe

scholarly journals Mitochondrial DNA Variation of Feral Honey Bees (Apis mellifera L.) from Utah (USA)

2018 ◽  
Vol 62 (2) ◽  
pp. 223-232
Author(s):  
Dylan Cleary ◽  
Allen L. Szalanski ◽  
Clinton Trammel ◽  
Mary-Kate Williams ◽  
Amber Tripodi ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Apis Mellifera ◽  
Honey Bee ◽  
Honey Bees ◽  
Western Europe ◽  
Dna Variation ◽  
Bee Colony ◽  
The Us ◽  
Coii Gene

Abstract A study was conducted on the mitochondrial DNA genetic diversity of feral colonies and swarms of Apis mellifera from ten counties in Utah by sequencing the intergenic region of the cytochrome oxidase (COI-COII) gene region. A total of 20 haplotypes were found from 174 honey bee colony samples collected from 2008 to 2017. Samples belonged to the A (African) (48%); C (Eastern Europe) (43%); M (Western Europe) (4%); and O (Oriental) lineages (5%). Ten African A lineage haplotypes were observed with two unique to Utah among A lineage haplotypes recorded in the US. Haplotypes belonging to the A lineage were observed from six Utah counties located in the southern portion of the State, from elevations as high as 1357 m. All five C lineage haplotypes that were found have been observed from queen breeders in the US. Three haplotypes of the M lineage (n=7) and two of the O lineage (n=9) were also observed. This study provides evidence that honey bees of African descent are both common and diverse in wild populations of honey bees in southern Utah. The high levels of genetic diversity of A lineage honey bee colonies in Utah provide evidence that the lineage may have been established in Utah before the introduction of A lineage honey bees from Brazil to Texas in 1990.

Aggressiveness and genetic diversity of Ralstonia solanacearum strains from tomato in Vietnam

2018 ◽  
Vol 71 (4) ◽  
pp. 599-610
Author(s):  
Hai Thi Hong Truong ◽  
Thanh-Thuy Duong ◽  
Tram Thi Hoai Nguyen ◽  
Thuy Thi Thu Nguyen ◽  
Linh Hoang Khanh Nguyen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ralstonia Solanacearum

scholarly journals Genetic diversity in portuguese native Vitis vinifera L. ssp. vinifera and ssp. sylvestris

2010 ◽  
Vol 46 (Special Issue) ◽  
pp. S54-S56 ◽  
Author(s):  
J. Cunha ◽  
M. Teixeira Santos ◽  
J. Brazăo ◽  
L.C. Carneiro ◽  
M. Veloso ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Genetic Structure ◽  
Vitis Vinifera ◽  
Gene Pool ◽  
Western Europe ◽  
Eastern European ◽  
Starting Point ◽  
Probable Origin ◽  
Different Origins

To assess the different origins of Portuguese grapevine varieties, we used six nuclear and four chloroplastidal microsatellites as molecular markers, in order to compare the genetic structure of native wild-vines with native grapevine varieties. Both native subspecies have a great diversity, and a high interrelationship across the six nuclear microsatellites. Although identical numbers of alleles were found in each population, their distribution was different in the <I>vinifera </I>and <I>sylvestris </I>subspecies. Portuguese wild-vines have only chlorotypes A and B; A being the most frequent. The fifty-seven analysed Portuguese varieties have chlorotypes A, B, C, and D. The most frequent was the chlorotype A (75%), followed by D (21%). The results obtained reinforced the idea of Western Europe as having been one of the domestication centres for the grapevine, with contributions from the Eastern European gene pool. The observed genetic structure is a starting point from which to clarify the high number of native cultivars found in Portugal, and reinforces their probable origin in the Iberian Peninsula.

scholarly journals Broad Diversity of Ralstonia solanacearum Strains in Cameroon

Plant Disease ◽  
2009 ◽  
Vol 93 (11) ◽  
pp. 1123-1130 ◽  
Author(s):  
Gabriel Mahbou Somo Toukam ◽  
Gilles Cellier ◽  
Emmanuel Wicker ◽  
Caroline Guilbaud ◽  
Rémi Kahane ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ralstonia Solanacearum ◽  
Central Africa ◽  
Sequence Analyses ◽  
Chain Reaction ◽  
Specific Pcr ◽  
Economic Threat ◽  
The Indian Ocean ◽  
Polymerase Chain ◽  
Reference Strains

In 2005, an extensive survey of bacterial wilt in Cameroon collected 110 strains of Ralstonia solanacearum from wilting tomato, potato, pepper, huckleberry (Solanum scabrum), sesame, and amaranth. The genetic diversity and phylogeny of selected strains from Cameroon were assessed by multiplex–polymerase chain reaction (PCR), race 3/biovar 2–specific PCR, and sequence analyses of the mutS and egl genes. These data were compared with those from 33 reference strains covering the known diversity within the R. solanacearum species complex. Strains isolated in Cameroon clustered into three of the four known phylotypes: I (Asian), II (American), and III (African). Lowland tomato strains belonged to phylotype I and were quite homogeneous. The strains belonging to phylotype II were genetically diverse, and partitioned into subclusters IIA and IIB (sequevar 1, race 3/biovar 2). Cameroon strains in the African phylotype III were distinct from reference strains from Zimbabwe or the Indian Ocean, highlighting the genetic diversity present within this phylotype. Strains from potatoes growing in the highlands of West Cameroon fell into both phylotypes II (race 3/biovar 2) and III. These phylotype II and III highland strains attacked both potato and tomato and could therefore pose an economic threat to potato and tomato crops throughout Central Africa. This is the first comprehensive report on the genetic diversity of R. solanacearum strains in Cameroon.

scholarly journals Genetic Diversity and Aggressiveness of Ralstonia solanacearum Strains Causing Bacterial Wilt of Potato in Uruguay

Plant Disease ◽  
2011 ◽  
Vol 95 (10) ◽  
pp. 1292-1301 ◽  
Author(s):  
M. I. Siri ◽  
A. Sanabria ◽  
M. J. Pianzzola
Keyword(s):  
Genetic Diversity ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Potato Tubers ◽  
Bacterial Strains ◽  
Potato Plants ◽  
Polymerase Chain ◽  
First Time ◽  
Different Levels

Bacterial wilt, caused by Ralstonia solanacearum, is a major disease affecting potato (Solanum tuberosum) production worldwide. Although local reports suggest that the disease is widespread in Uruguay, characterization of prevalent R. solanacearum strains in that country has not been done. In all, 28 strains of R. solanacearum isolated from major potato-growing areas in Uruguay were evaluated, including 26 strains isolated from potato tubers and 2 from soil samples. All strains belonged to phylotype IIB, sequevar 1 (race 3, biovar 2). Genetic diversity of strains was assessed by repetitive-sequence polymerase chain reaction, which showed that the Uruguayan strains constituted a homogeneous group. In contrast, inoculation of the strains on tomato and potato plants showed, for the first time, different levels of aggressiveness among R. solanacearum strains belonging to phylotype IIB, sequevar 1. Aggressiveness assays were also performed on accessions of S. commersonii, a wild species native to Uruguay that is a source of resistance for potato breeding. No significant interactions were found between bacterial strains and potato and S. commersonii genotypes, and differences in aggressiveness among R. solanacearum strains were consistent with previously identified groups based on tomato and potato inoculations. Moreover, variation in responses to R. solanacearum was observed among the S. commersonii accessions tested.

scholarly journals Genetic Diversity of the Ralstonia solanacearum Species Complex in the Southwest Indian Ocean Islands

2017 ◽  
Vol 8 ◽  
Author(s):  
Noura Yahiaoui ◽  
Jean-Jacques Chéron ◽  
Santatra Ravelomanantsoa ◽  
Azali A. Hamza ◽  
Bobb Petrousse ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Indian Ocean ◽  
Ralstonia Solanacearum ◽  
Species Complex ◽  
Southwest Indian Ocean ◽  
Ocean Islands

scholarly journals Genetic Diversity of Zingiberaceae Plant Isolates of Ralstonia solanacearum in the Asia-Pacific Region

2013 ◽  
Vol 47 (3) ◽  
pp. 283-294 ◽  
Author(s):  
Takamitsu WAKI ◽  
Mitsuo HORITA ◽  
Daisuke KUROSE ◽  
Karden MULYA ◽  
Kenichi TSUCHIYA
Keyword(s):  
Genetic Diversity ◽  
Ralstonia Solanacearum ◽  
Asia Pacific ◽  
Pacific Region ◽  
Asia Pacific Region

scholarly journals Drivers of genetic differentiation and recent evolutionary history of an Eurasian wild pea

2021 ◽  
Author(s):  
Timo Hellwig ◽  
Shahal Abbo ◽  
Ron Ophir
Keyword(s):  
Genetic Diversity ◽  
Land Use ◽  
Pisum Sativum ◽  
Genetic Differentiation ◽  
Range Expansion ◽  
Evolutionary History ◽  
Western Europe ◽  
Genotyping By Sequencing ◽  
Population Recovery ◽  
Environmental Predictors

Genetic diversity a major determinant for the capacity of species to persist and adapt to their environments. Unraveling the factors affecting genetic differentiation is crucial to understand how genetic diversity is shaped and species may react to changing environments. We employed genotyping by sequencing to test the influence of climate, space, latitude, altitude and land cover on genetic differentiation in a collection of 81 wild pea samples (Pisum sativum ssp. elatius) from across its distribution range from western Europe to central Asia. We also attempted to elucidate the species recent evolutionary history and its effect on the current distribution of genetic diversity. Association of single SNPs with climate variables were analyses to test for signatures of local adaptation. Genetic variation was geographically structured into six distinct genetic cluster. Two of which were associated with a taxonomic group (Pisum sativum ssp. humile) that according to some researchers does not qualify for a sub-species rank due to its alleged lack of genetic distinctness from other conspecific groups. The effect of the tested factors influencing genetic differentiation were rather variable among genetic clusters. The climate predictors were most important in all clusters. Land use was more important in clusters from areas strongly influenced by human land use, especially by agriculture. We found a statistically significant association of 3,623 SNPs (2.4 % of all SNPs) with one of the environmental predictors. Most of them were correlated with latitude followed by temperature, precipitation and altitude. Estimation of SNP effects of the candidates resulted in a missense to silent ratio of 0.45, suggesting many of the observed candidates SNPs may alter the encoded amino acid sequence. Wild peas went through a genetic bottleneck during the last glacial period followed by population recovery. Probably associated with this population recovery, we detected a range expansion, which may have led to an eastward range expansion of the European cluster to Turkey and thereof southwards and eastwards. Overall, the interplay of several environmental factors and the recent evolutionary history affected the distribution of genetic diversity in wild peas where each subpopulations were differently affected by those factors and processes.

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