scholarly journals Inhibition of the filamentation of Candida albicans by Borojoa patinoi silver nanoparticles

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Marcela Gómez-Garzón ◽  
Luz D. Gutiérrez-Castañeda ◽  
Camilo Gil ◽  
Carlos H. Escobar ◽  
Ana P. Rozo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Candida Albicans ◽  
Green Synthesis ◽  
Cell Line ◽  
Opportunistic Pathogen ◽  
Inhibitory Effect ◽  
Cell Toxicity ◽  
Toxicity Assay ◽  
Filamentous Form ◽  
Shape And Size

AbstractCandida albicans is fungus capable of changing from yeast to filamentous form when it’s transformed from a normal commensal to an opportunistic pathogen. The development of alternatives that interfere with this transition could be an effective way to reduce candidiasis. In this regard, evaluate the inhibitory effect of two Borojoa patinoi silver nanoparticles (AgNPs) produced by green synthesis at 5 °C and 25 °C on the process of filamentation of Candida albicans. The percentage of inhibition of filamentous forms of C. albicans ATCC10231 and C. albicans SC5314 with AgNPs was determined. Results showed that temperature of synthesis affected both the shape and size of silver nanoparticles synthesized using Borojoa patinoi extracts. The inhibition percentage of filamentous forms of Candida albicans ATCC10231 when treated with silver nanoparticles synthesized at 5 °C was 85.9% and at 25 °C it was 40%. C. albicans SC5314 when treated with AgNP synthesized at 5 °C was 97.2% and at 25 °C it was 64%. Cell toxicity assay showed that at 100ng/ml, AgNPs synthesized at 25 °C were safe in MES-OV CRL-3272 cell line. Our results showed that the silver nanoparticles obtained from Borojoa patinoi are inhibitors of the filamentous process of C. albicans.

Green synthesis, characterization and antibacterial activity of silver nanoparticles by Malus domestica and its cytotoxic effect on (MCF-7) cell line

2019 ◽  
Vol 135 ◽  
pp. 103609 ◽  
Author(s):  
Arokia Vijaya Anand Mariadoss ◽  
Vinayagam Ramachandran ◽  
Vijayakumar Shalini ◽  
Balupillai Agilan ◽  
Jebaraj Herbert Franklin ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Cell Line ◽  
Malus Domestica ◽  
Cytotoxic Effect ◽  
Mcf 7

Green Synthesis and Characterization of Silver Nanoparticles for Antimicrobial Activity Against Burn Wounds Contaminating Bacteria

2014 ◽  
Vol 13 (02) ◽  
pp. 1450010
Author(s):  
Anandini Rout ◽  
Padan K. Jena ◽  
Debasish Sahoo ◽  
Umesh K. Parida ◽  
Birendra K. Bindhani
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Inhibitory Effect ◽  
Atmospheric Conditions ◽  
Burn Wound ◽  
X Ray ◽  
Mueller Hinton ◽  
Liquid Systems ◽  
Mueller Hinton Agar

Silver nanoparticles ( AgNPs ) were prepared from the plant extract of N. arbor-tristis under atmospheric conditions through green synthesis and characterized by various physicochemical techniques like UV-Visible spectroscopy, IR Spectra, energy dispersive X-ray spectrometry (EDS), X-ray diffraction and transmission electron microscopy (TEM) and the results confirmed the synthesis of homogeneous and stable AgNPs by the plant extracts. The antimicrobial activity of AgNPs was investigated against most common bacteria found in burn wound Staphylococcus epidermidis and Pseudomonas aeruginosa. In these tests, Mueller Hinton agar plates were used with AgNPs of various concentrations, supplemented in liquid systems. P. aeruginosa was inhibited at the low concentration of AgNPs , whereas the growth-inhibitory effect on S. epidermidis was mild. These results suggest that AgNPs can be used as effective growth inhibitors of various microorganisms, making them applicable to diverse medical devices and antimicrobial control systems.

Rapid recruitment of late endosomes and lysosomes in mouse macrophages ingesting Candida albicans

1999 ◽  
Vol 112 (19) ◽  
pp. 3237-3248 ◽  
Author(s):  
R. Kaposzta ◽  
L. Marodi ◽  
M. Hollinshead ◽  
S. Gordon ◽  
R.P. da Silva
Keyword(s):  
Candida Albicans ◽  
Mannose Receptor ◽  
Opportunistic Pathogen ◽  
Tube Formation ◽  
Heat Inactivation ◽  
Filamentous Form ◽  
Late Endosomes ◽  
Latex Beads ◽  
Confocal Immunofluorescence ◽  
Mouse Macrophages

Candida albicans is an important opportunistic pathogen, whose interaction with cells of the immune system, in particular macrophages (MO), is poorly understood. In order to learn more about the nature of the infectious mechanism, internalisation of Candida albicans was studied in mouse MO by confocal immunofluorescence and electron microscopy in comparison with latex beads of similar size, which were coated with mannosyl-lipoarabinomannan (ManLAM) to target the MO mannose receptor (MR). Uptake of Candida yeasts had characteristics of phagocytosis, required intact actin filaments, and depended on the activity of protein kinase C (PKC). Candida phagosomes rapidly attracted lysosome-associated membrane protein (Lamp)-rich vacuoles, indicative of fusion with late endosomes and lysosomes. Rapid recruitment of late endosomes and lysosomes could be observed regardless of heat-inactivation or serum-opsonisation of Candida, but did not follow binding of the mannosylated-beads to MO, which suggest that this phenotype is not MR-specific. The yeasts developed germ tubes within phagolysosomes, distended their membranes and escaped, destroying the non-activated MO. The filamentous form of Candida could penetrate intact MO even when phagocytosis was blocked, and also attracted Lamp-rich organelles. Inhibition of lysosomal acidification and associated lysosomal fusion reduced germ tube formation of Candida within the phagolysosomes. These data suggest that rapid recruitment of late endocytic/lysosomal compartments by internalizing C. albicans favours survival and virulence of this pathogen.

scholarly journals Green Synthesis of Gold and Silver Nanoparticles by Using Amorphophallus paeoniifolius Tuber Extract and Evaluation of Their Antibacterial Activity

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4773
Author(s):  
S. M. Abu Nayem ◽  
Nasrin Sultana ◽  
Md. Aminul Haque ◽  
Billal Miah ◽  
Md. Mahmodul Hasan ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Inhibitory Effect ◽  
Reducing Agent ◽  
Gram Negative Bacteria ◽  
Gold And Silver Nanoparticles ◽  
Gram Negative ◽  
X Ray ◽  
Tuber Extract

In this report, we discussed rapid, facile one-pot green synthesis of gold and silver nanoparticles (AuNPs and AgNPs) by using tuber extract of Amorphophallus paeoniifolius, and evaluated their antibacterial activity. AuNPs and AgNPs were synthesized by mixing their respective precursors (AgNO3 and HAuCl4) with tuber extract of Amorphophallus paeoniifolius as the bio-reducing agent. Characterization of AuNPs and AgNPs were confirmed by applying UV-vis spectroscopy, field-emission scanning electron microscopy (FESEM), X-ray diffraction (XRD) analysis, Fourier transform infrared spectroscopy (FTIR), and energy dispersive X-ray spectroscopy (EDS). From UV-vis characterization, surface plasmon resonance spectra were found at 530 nm for AuNPs and 446 nm for AgNPs. XRD data confirmed that both synthesized nanoparticles were face-centered cubic in crystalline nature, and the average crystallite sizes for the assign peaks were 13.3 nm for AuNPs and 22.48 nm for AgNPs. FTIR data evaluated the characteristic peaks of different phytochemical components of tuber extract, which acted as the reducing agent, and possibly as stabilizing agents. The antibacterial activity of synthesized AuNPs and AgNPs were examined in Muller Hinton agar, against two Gram-positive and four Gram-negative bacteria through the disc diffusion method. AuNPs did not show any inhibitory effect, while AgNPs showed good inhibitory effect against both Gram-positive and Gram-negative bacteria.

Green Synthesis of Silver Nanoparticles Using Aqueous Extract of Lamium album and their Antifungal Properties

2021 ◽  
Vol 67 ◽  
pp. 55-67
Author(s):  
Zahra Zareshahrabadi ◽  
Fatemeh Karami ◽  
Saeed Taghizadeh ◽  
Aida Iraji ◽  
Ali Mohammad Amani ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Candida Albicans ◽  
Green Synthesis ◽  
Aqueous Extract ◽  
Inhibitory Activity ◽  
Biofilm Formation ◽  
Dependent Manner ◽  
Face Centered Cubic ◽  
Antifungal Activities ◽  
Lamium Album

Biosynthesis of novel metal nanoparticles (especially silver) using plant derivatives has received increasing attention due to their eco-friendly and potential applications in pharmaceutical and medical fields. In this study, silver nanoparticles were synthesized by using aqueous extract of Lamium album as a biocompatible and green method. The synthesized silver nanoparticles were characterized by Ultraviolet-visible (UV–vis) Spectroscopy, Transmission Electron Microscopy (TEM), X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), and Dynamic Light Scattering (DLS) Analysis. Inhibitory activity of silver nanoparticles on fungal growth was evaluated and inhibition of Candida albicans biofilms formation was measured using XTT assay. Their antioxidant and cytotoxic properties have also been evaluated.The synthesized AgNPs were mostly spherical in shape with an average size of nearly 25.2 nm. The AgNPs were crystalline in nature and have a face-centered cubic structure. The results of zeta potential value for AgNPs were −30 mV indicating the normal stability of the synthesized nanoparticles in colloidal systems. The result of antifungal activities showed that the biosynthesized silver nanoparticles had inhibitory activity against Candida albicans, C. tropicalis, C. krusei, C. glabrata, C. dubliniensis, C. parapsilosis, Cryptococcus neoformance, Aspergillus flavus, A. clavatus, Aspergillus fumigatus, Pseudallescheria boydii and Exophiala dermatitidis. The nanoparticles inhibited the Candida albicans biofilm formation in a dose-dependent manner. The cytotoxicity study of silver nanoparticles was revealed IC50 of 110.75 μg/mL against liver cell lines. The recognized bioactivity confirmed by the synthesized silver nanoparticles directs towards the potential for using as an antioxidant, antifungal, and cytotoxic agent.Keywords: Green synthesis, Antifungal activities, Silver nanoparticles, Lamium album, Biofilm formation.

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