Indole acetic acid (IAA) producing endophytic bacteria on direct somatic embryogenesis and plant regeneration of Exacum travancoricum Bedd.

2020 ◽  
Vol 33 (4) ◽  
pp. 690-702
Author(s):  
Chellappan Soundar Raju ◽  
Abubakker Aslam ◽  
Devarajan Thangadurai ◽  
Jeyabalan Sangeetha ◽  
Krishnan Kathiravan ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Direct Somatic Embryogenesis

Isolation, Culture and Plant Regeneration From Protoplasts of Nicotiana debneyi

1980 ◽  
Vol 7 (6) ◽  
pp. 635 ◽  
Author(s):  
WR Scowcroft ◽  
PJ Larkin
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Indole Acetic Acid ◽  
High Efficiency ◽  
Protoplast Culture ◽  
Callus Cultures ◽  
Dna Uptake ◽  
Mesophyll Protoplasts ◽  
Nicotiana Debneyi ◽  
Protoplast Division

Mesophyll protoplasts of two genetically distinct genotypes of N. debneyi were cultured with sustained division following a plating efficiency in excess of 50%. Fully fertile mature plants were regenerated from callus cultures derived from protoplasts. Shoots were induced in medium containing 1 mg/l 6-benzylaminopurine and 0.5 mg/I indole acetic acid. The repeatably high efficiency of protoplast culture was used to evaluate the quantitative effects of two drugs, kanamycin and trimethoprim, which effectively inhibited colony formation at concentrations of 100 and 50 �g/ml, respectively. An enhancer of DNA uptake, poly-L-ornithine, had virtually no effect on sustained protoplast division at a concentration of 7.5 �g/ml or less.

scholarly journals Bakteri Endofit Tanaman Jeruk Nipis (Citrus aurantifolia) Penghasil Asam Indol Asetat (AIA)

2020 ◽  
Vol 13 (2) ◽  
pp. 179-191
Author(s):  
Oktira Roka Aji ◽  
Iva Dita Lestari
Keyword(s):  
Acetic Acid ◽  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Biochemical Analysis ◽  
Crop Productivity ◽  
Bacterial Identification ◽  
Test Results ◽  
Bacterial Isolates ◽  
Characteristic Analysis ◽  
Citrus Aurantifolia

AbstrakBakteri endofit hidup dalam suatu tanaman tanpa menyebabkan gangguan bagi tanaman yang berperan penting dalam menstimulasi pertumbuhan tanaman, yaitu dengan memproduksi fitohormon seperti asam absisat, asam indol asetat, dan sitokinin. Penelitian ini bertujuan untuk mengisolasi, menyeleksi, dan mengidentifikasi bakteri endofit yang terdapat pada daun, batang, dan akar tanaman jeruk nipis (Citrus aurantifolia). Isolat bakteri endofit diseleksi berdasarkan kemampuannya dalam menghasilkan asam indol asetat (AIA). Isolat bakteri endofit ditumbuhkan pada media nutrient broth (NB) yang ditambah dengan L-triptofan. Konsentrasi AIA dihitung dengan penambahan reagen salkowski dan diukur menggunakan spektrofotometer pada panjang gelombang 530 nm. Identifikasi bakteri endofit dilakukan dengan analisis uji biokimia. Isolat bakteri endofit yang berhasil diisolasi sebanyak 12 isolat, yaitu 4 isolat dari daun, 4 isolat dari batang, dan 4 isolat dari akar. Hasil pengamatan pada uji AIA menunjukkan bahwa semua isolat bakteri endofit dapat menghasilkan hormon AIA. Isolat yang menghasilkan konsentrasi hormon AIA tertinggi adalah isolat B2 (6,51 ppm). Isolat bakteri yang berhasil diidentifikasi berasal dari genus Enterobacter, Bacillus, Pseudomonas, dan Staphylococcus. Bakteri endofit yang dapat menghasilkan AIA berpotensi dikembangkan sebagai biofertilizer untuk meningkatkan produktivitas tanaman. Abstract Endophytic bacteria live inside plants without causing disruption to plants and play an important role in stimulating plant growth. This study aims to isolate endophytic bacteria from lime plant (Citrus aurantifolia) and characterize its ability to produce indole acetic acid (IAA). Bacterial isolates were grown on media supplemented with L-tryptophan as IAA precursor. The bacterial supernatant was mixed with salkowski reagents and then measured using a spectrophotometer at 530 nm. Bacterial identification was carried out using biochemical characteristic analysis. A total of 12 endophytic bacterial isolates were successfully isolated from leaves, stem and roots of plants. Quantitative test results showed that all isolates can produce IAA. The highest concentration of IAA was produced by B2 (6.51 ppm). Biochemical analysis indicated that the isolates were from the genus Enterobacter, Bacillus, Pseudomonas and Staphylococcus. Endhophytic bacteria that can produce IAA have the potential to be developed as biofertilizers to increase crop productivity.

Direct somatic embryogenesis and plant regeneration from immature explants of chickpea

2010 ◽  
Vol 54 (1) ◽  
pp. 121-125 ◽  
Author(s):  
S. Ghanti ◽  
K. G. Sujata ◽  
M. Rao ◽  
P. B. Kishor
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Direct Somatic Embryogenesis

Direct somatic embryogenesis and plant regeneration from protoplasts of red clover (Trifolium pratense L.)

Plant Science ◽  
1994 ◽  
Vol 97 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Maxim A. Radionenko ◽  
Nikolay V. Kuchuk ◽  
Olga A. Khvedynich ◽  
Yuri Yu. Gleba
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Direct Somatic Embryogenesis ◽  
Trifolium Pratense L

scholarly journals NAA at a high concentration promotes efficient plant regeneration via direct somatic embryogenesis and SE-mediated transformation system in Ranunculus sceleratus

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ke-dong Xu ◽  
Wei Wang ◽  
De-shui Yu ◽  
Xiao-li Li ◽  
Jia-min Chen ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Marker Gene ◽  
Direct Somatic Embryogenesis ◽  
The Novel ◽  
Transformation System ◽  
High Concentration ◽  
High Frequencies ◽  
Classical Pattern ◽  
Flower Organs

AbstractThe novel methods for efficient plant regeneration via direct somatic embryogenesis (SE) and SE-mediated transformation system under high concentration of NAA in Ranunculus sceleratus were established. On MS media containing a high concentration of NAA (10.0 mg/L) in the dark, all inoculated explants (root, stem and leaf) formed somatic embryos at high frequencies, respectively, 66.03, 126.47 and 213.63 embryoids per explant, and 100% of the embryoids developed into plantlets on 1/2 MS rooting media. Morphological and histological analyses revealed that SE in R. sceleratus followed a classical pattern. All inoculated explants can be used as receptors for genetic transformation in R. sceleratus, through direct SE-mediated method after Agrobacterium infection. RcLEC1-B, as a marker gene, changed the number and morphology of flower organs and the development of cuticle in R. sceleratus, which indicated that the efficient transgenic system of R. sceleratus was established. To our knowledge, this is the first observation that both direct SE and transgenic transformation system, via induction of a single plant growth regulator, have been successfully constructed in R. sceleratus.

scholarly journals Uji Potensi Bakteri Endofit Kitinolitik Tanaman Padi (Oryza sativa L.) Sebagai Penghasil Hormon IAA (Indole Acetic Acid)

2017 ◽  
Vol 19 (1) ◽  
pp. 76
Author(s):  
Ahmad Hanafi ◽  
Susiana Purwantisari ◽  
Budi Raharjo
Keyword(s):  
Acetic Acid ◽  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Oryza Sativa L ◽  
Block Design ◽  
Outcome Data ◽  
Bacterial Isolates ◽  
Treatment Combination ◽  
Measured Variable ◽  
Tryptophan Concentration

IAA (Indole Acetic Acid) is a hormone in plants that was role in the cleavage of roots, inhibits the growth of side shoots, stimulate cell division and the formation of xylem and phloem tissue. This study aimed to test the potential of endophytic bacteria chitinolytic rice crop as hormone-producing IAA. This study uses 9 isolates of endophytic bacteria chitinolytic rice plants in isolation during practical work. The experiment consisted of 15 treatments and 3 replications. This study uses a randomized block design. The treatments tryptophan concentration combined with a variation pH, the endophytic bacteria grown on media chitinolytic tryptophan concentration of 0 mg/L, 102 mg/L, 204 mg/L, 306 mg/L and 408 mg/L are combined with pH 5, 7 and 9. the treatment was observed for 48 hours and observation once every 3 hours. The measured variable is the result of the production of IAA hormone with the treatment combination of tryptophan with pH. IAA hormone outcome data were analyzed using Analysis of Variance Univariates at level of 95%. IAA hormone qualitative test results showed positive results in bacterial isolates KA12, KA11 and KB24. IAA hormone quantitative results of bacterial isolates producing IAA hormone KA12 high of 2,03 mg/L in the combination treatment of tryptophan 408 mg/L at pH 7 at 24 hours incubation. KA12 bacterial isolates are endophytic bacteria chitinolytic potential to produce hormones IAA, yet the results of data analysis showed that each treatment combination with pH tryptophan to IAA production were not significantly different. Keywords: hormone IAA, chitinolytic endophytic bacteria, tryptophan, pH

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