scholarly journals Uji Potensi Bakteri Endofit Kitinolitik Tanaman Padi (Oryza sativa L.) Sebagai Penghasil Hormon IAA (Indole Acetic Acid)

2017 ◽  
Vol 19 (1) ◽  
pp. 76
Author(s):  
Ahmad Hanafi ◽  
Susiana Purwantisari ◽  
Budi Raharjo
Keyword(s):  
Acetic Acid ◽  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Oryza Sativa L ◽  
Block Design ◽  
Outcome Data ◽  
Bacterial Isolates ◽  
Treatment Combination ◽  
Measured Variable ◽  
Tryptophan Concentration

IAA (Indole Acetic Acid) is a hormone in plants that was role in the cleavage of roots, inhibits the growth of side shoots, stimulate cell division and the formation of xylem and phloem tissue. This study aimed to test the potential of endophytic bacteria chitinolytic rice crop as hormone-producing IAA. This study uses 9 isolates of endophytic bacteria chitinolytic rice plants in isolation during practical work. The experiment consisted of 15 treatments and 3 replications. This study uses a randomized block design. The treatments tryptophan concentration combined with a variation pH, the endophytic bacteria grown on media chitinolytic tryptophan concentration of 0 mg/L, 102 mg/L, 204 mg/L, 306 mg/L and 408 mg/L are combined with pH 5, 7 and 9. the treatment was observed for 48 hours and observation once every 3 hours. The measured variable is the result of the production of IAA hormone with the treatment combination of tryptophan with pH. IAA hormone outcome data were analyzed using Analysis of Variance Univariates at level of 95%. IAA hormone qualitative test results showed positive results in bacterial isolates KA12, KA11 and KB24. IAA hormone quantitative results of bacterial isolates producing IAA hormone KA12 high of 2,03 mg/L in the combination treatment of tryptophan 408 mg/L at pH 7 at 24 hours incubation. KA12 bacterial isolates are endophytic bacteria chitinolytic potential to produce hormones IAA, yet the results of data analysis showed that each treatment combination with pH tryptophan to IAA production were not significantly different. Keywords: hormone IAA, chitinolytic endophytic bacteria, tryptophan, pH

scholarly journals Bakteri Endofit Tanaman Jeruk Nipis (Citrus aurantifolia) Penghasil Asam Indol Asetat (AIA)

2020 ◽  
Vol 13 (2) ◽  
pp. 179-191
Author(s):  
Oktira Roka Aji ◽  
Iva Dita Lestari
Keyword(s):  
Acetic Acid ◽  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Biochemical Analysis ◽  
Crop Productivity ◽  
Bacterial Identification ◽  
Test Results ◽  
Bacterial Isolates ◽  
Characteristic Analysis ◽  
Citrus Aurantifolia

AbstrakBakteri endofit hidup dalam suatu tanaman tanpa menyebabkan gangguan bagi tanaman yang berperan penting dalam menstimulasi pertumbuhan tanaman, yaitu dengan memproduksi fitohormon seperti asam absisat, asam indol asetat, dan sitokinin. Penelitian ini bertujuan untuk mengisolasi, menyeleksi, dan mengidentifikasi bakteri endofit yang terdapat pada daun, batang, dan akar tanaman jeruk nipis (Citrus aurantifolia). Isolat bakteri endofit diseleksi berdasarkan kemampuannya dalam menghasilkan asam indol asetat (AIA). Isolat bakteri endofit ditumbuhkan pada media nutrient broth (NB) yang ditambah dengan L-triptofan. Konsentrasi AIA dihitung dengan penambahan reagen salkowski dan diukur menggunakan spektrofotometer pada panjang gelombang 530 nm. Identifikasi bakteri endofit dilakukan dengan analisis uji biokimia. Isolat bakteri endofit yang berhasil diisolasi sebanyak 12 isolat, yaitu 4 isolat dari daun, 4 isolat dari batang, dan 4 isolat dari akar. Hasil pengamatan pada uji AIA menunjukkan bahwa semua isolat bakteri endofit dapat menghasilkan hormon AIA. Isolat yang menghasilkan konsentrasi hormon AIA tertinggi adalah isolat B2 (6,51 ppm). Isolat bakteri yang berhasil diidentifikasi berasal dari genus Enterobacter, Bacillus, Pseudomonas, dan Staphylococcus. Bakteri endofit yang dapat menghasilkan AIA berpotensi dikembangkan sebagai biofertilizer untuk meningkatkan produktivitas tanaman. Abstract Endophytic bacteria live inside plants without causing disruption to plants and play an important role in stimulating plant growth. This study aims to isolate endophytic bacteria from lime plant (Citrus aurantifolia) and characterize its ability to produce indole acetic acid (IAA). Bacterial isolates were grown on media supplemented with L-tryptophan as IAA precursor. The bacterial supernatant was mixed with salkowski reagents and then measured using a spectrophotometer at 530 nm. Bacterial identification was carried out using biochemical characteristic analysis. A total of 12 endophytic bacterial isolates were successfully isolated from leaves, stem and roots of plants. Quantitative test results showed that all isolates can produce IAA. The highest concentration of IAA was produced by B2 (6.51 ppm). Biochemical analysis indicated that the isolates were from the genus Enterobacter, Bacillus, Pseudomonas and Staphylococcus. Endhophytic bacteria that can produce IAA have the potential to be developed as biofertilizers to increase crop productivity.

scholarly journals GROWTH IMPROVEMENT OF TOMATO WITH THE APPLICATION OF BACTERIAL ISOLATES PRODUCING INDOLE ACETIC ACID (IAA) AND PHOSPHATE SOLUBILIZER

2019 ◽  
Vol 20 (1) ◽  
pp. 35
Author(s):  
Eny Ida Riyanti ◽  
Dwi Ningsih Susilowati ◽  
Karden Mulya ◽  
Edy Listanto
Keyword(s):  
Acetic Acid ◽  
Root Length ◽  
Crop Production ◽  
Indole Acetic Acid ◽  
Dry Weight ◽  
Fruit Yield ◽  
Bacterial Isolates ◽  
Phenotypic Properties ◽  
Root Dry Weight ◽  
Tomato Growth

<p class="keyword">Soil bacteria have important roles in biogeochemical cycle for soil fertility and have been manipulated for ecologically-friendly crop production.  The search for beneficial association between microbes and plants for promoting growth and health should be studied for tomato growth improvement. The study aimed to  evaluate 19 microbial isolates which produced indole acetic acid (IAA) affecting growth and development of tomato (Palupi variety), and  molecularly identify the most effective isolates in improving tomato growth based on 16s rDNA sequences. The experiment was conducted in pots using a complete randomized design with three replications. The parameters observed included plant height, plant dry weight, root length, root dry weight, and fruit fresh weight.  The isolates that significantly improved tomato growth were molecularly identified using 16s rRNA sequence. The phenotypic properties such as IAA content and phosphate solubilizing index (PI) of the superior isolates were determined. Results showed that the application of bacterial isolates on tomato significantly increased plant dry weight and fruit yield. From 19 isolates tested, Aj 3.7.1.14 significantly increased plant dry weight, root length, and fruit yield. This isolate produced IAA of about 14.77 ppm and PI of 1.86.  Molecular analysis on Aj 3.7.1.14 demonstrated that the isolate had 89% similarity to <em>Pseudomonas fragi</em>. The identified <em>P. fragi</em> was found to be the most effective isolate for improving tomato growth and fruit yield. Another isolate, <em>Bacillus amyloliquefaciens</em> was found to promote root length, root dry weight, and fruit yield. These isolates are potential to be further investigated for field trials</p>

scholarly journals Pengaruh Pupuk Hayati Endofitik Dengan Azolla Pinnata Terhadap Serapan N , N-Total Tanah, Dan Bobot Kering Tanaman Padi (Oryza Sativa L.) Pada Tanah Salin

Agrologia ◽  
2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Alin Kusumah Dewi ◽  
Mieke Rochimi Setiawati
Keyword(s):  
Nitrogen Uptake ◽  
Endophytic Bacteria ◽  
Saline Soil ◽  
Oryza Sativa L ◽  
Block Design ◽  
Nitrogen Deficiency ◽  
Dry Weight ◽  
Biological Properties ◽  
Azolla Pinnata ◽  
Total N

Saline soil contain excessive NaCl content which inhibit plant growth and lead to nitrogen deficiency. Endophytic bacteria live inside the plant tissue and has the ability to fix nitrogen directly from the atmosphere, meanwhile Azolla pinnata can be used as organic ameliorant that able to increase the number of availability nitrogen for plant in soil, and improve the physical, chemical, and biological properties of soil. The aims of this experiment were to find out the treatment that cause highest nitrogen uptake by plant, total-N of soil, and dry weight of rice in saline soil. The experimental design was randomized block design with eight treatments and four repetitions. The results showed that the application of biofertilizer and ameliorant increased nitrogen uptake by plant and dry weight of rice in saline soil, but there was no significant effect on  total-N of soil. This experiment suggested that endophytic bacteria biofertilizer and Azolla pinnata were effective to increase dry weight and nitrogen uptake of rice grown in soil with EC between  2 dS m-1 - 4 dS m-1.

Indole acetic acid (IAA) producing endophytic bacteria on direct somatic embryogenesis and plant regeneration of Exacum travancoricum Bedd.

2020 ◽  
Vol 33 (4) ◽  
pp. 690-702
Author(s):  
Chellappan Soundar Raju ◽  
Abubakker Aslam ◽  
Devarajan Thangadurai ◽  
Jeyabalan Sangeetha ◽  
Krishnan Kathiravan ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Direct Somatic Embryogenesis

scholarly journals Quantitative assay of Indole Acetic Acid-producing bacteria isolated from several lakes in East Java, Indonesia

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Sulistya Ika Ramadhani Sulistya ◽  
Sitoresmi Prabaningtyas Sitoresmi ◽  
Agung Witjoro Agung ◽  
Rina Triturani Saptawati Rina ◽  
Achmad Rodiansyah Achmad
Keyword(s):  
Acetic Acid ◽  
Indole Acetic Acid ◽  
Fossil Fuels ◽  
Morphological Characterization ◽  
Biofuel Production ◽  
Quantitative Assay ◽  
Phenotypic Characterization ◽  
Bacterial Isolates ◽  
Acid Producing Bacteria

Abstract. Ramadhani SI, Prabaningtyas S, Witjoro A, Saptawati TR, Rodiansyah A. 2020. Quantitative assay of Indole Acetic Acid-producing bacteria isolated from several lakes in East Java, Indonesia. Biodiversitas 21: 5448-5454. Biofuel is an alternative to fossil fuels that are environmentally friendly with low emissions. Biofuel from biomass microalgae, especially Chlorella vulgaris, has an essential role in biofuel production. Increasing biomass microalgae was done by co-culture between microalgae and bacteria. This research aims to determine the potential of bacterial isolates to produce the IAA hormone and identify the highest isolate with the ability to synthesis IAA from four lakes in East Java. This research was conducted by culturing bacterial isolates in the Tryptic Soy Broth (TSB) to add tryptophan media in various periods of incubation. The absorbance was measured with UV-Vis spectrophotometry at a wavelength of 530 nm for determining IAA-production from bacterial isolates. The results showed that the "12" code bacterial isolate from Ranu Grati produced the highest IAA hormone concentration, with an average of 30.23 ppm. The morphological characterization of the highest IAA-producing bacteria showed that isolate included the Enterobacteriaceae group and phenotypic characterization include Enterobacter cloacae complex (ECC).

scholarly journals Indole acetic acid and ACC deaminase from endophytic bacteria improves the growth of Solanum lycopersicum

2016 ◽  
Vol 21 ◽  
pp. 58-64 ◽  
Author(s):  
Abdul Latif Khan ◽  
Boshra Ahmed Halo ◽  
Ali Elyassi ◽  
Sajid Ali ◽  
Khadija Al-Hosni ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Solanum Lycopersicum ◽  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Acc Deaminase

scholarly journals Effect of naphthalene acetic acid on sprouting and rooting of stem cutting in Mussaenda species

2019 ◽  
Vol 30 (1) ◽  
pp. 48-54
Author(s):  
MHA Rashid ◽  
A Nahar
Keyword(s):  
Acetic Acid ◽  
Root Formation ◽  
Block Design ◽  
Stem Cutting ◽  
Naphthalene Acetic Acid ◽  
Combined Effects ◽  
Stem Cuttings ◽  
Treatment Combination ◽  
Red Flag ◽  
Number Of Leaves

Mussaendas are increasingly popular for the showy colour they provide during much of the year in garden landscapes. However, root formation in stem cuttings is a major challenge for cultivation of Mussaenda species. Therefore, the experiment was carried out at the Landscaping section of the Department of Horticulture, Bangladesh Agricultural University, Mymensingh during the period from August to November 2018 to study the effect of phytohormone naphthalene acetic acid (NAA) on sprouting and rooting of stem cuttings in Mussaenda species. The two-factor experiment consisted of three Mussaenda species viz., Mussaenda frondosa (White flag bush), Mussaenda philippica (Pink flag bush) and Mussaenda erythrophylla (Red flag bush), and five concentrations of NAA viz., 0 (control), 0.1%, 0.2%, 0.3% and 0.4%. The experiment was laid out in randomised complete block design (RCBD) with three replications. The results revealed that all the traits related to sprouting and rooting potential of stem cuttings were significantly influenced by combined effects of Mussaenda species and various NAA concentrations. The maximum sprouted bud per cutting (3.57), highest number of roots per cutting (5.88) and the longest root length (4.32 cm) were recorded from M. philippica except number of leaves per stem cutting (4.42) in M. erythrophylla. The increasing concentrations of NAA application responded positively on sprouting and rooting characters in all three Mussaenda species used for this experiment. The best results obtained from the treatment where NAA was applied at the concentration of 0.3% with 3.92 sprouted buds per cutting, 4.77 leaves per cutting, 7.15 roots per cutting and 5.26 cm length of roots. The treatment combination of M. philippica (Pink flag bush) and 0.3% NAA, followed by NAA at 03% with M. erythrophylla (Red flag bush) was found to be best in terms of sprouting and rooting of stem cuttings in Mussaenda. Progressive Agriculture 30 (1): 48-54, 2019

scholarly journals Potensi Bakteri Endofit Ubi Jalar (Ipomoea batatas L.) dalam Menghasilkan Hormon Indole Acetic Acid (IAA) dengan Penambahan L-triptofan

2020 ◽  
Vol 10 (1) ◽  
pp. 21
Author(s):  
Agustina Monalisa Tangapo
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Sweet Potato ◽  
Growth Regulators ◽  
Endophytic Bacteria ◽  
Ipomoea Batatas ◽  
Indole Acetic Acid ◽  
Iaa Production ◽  
Promote Plant Growth ◽  
Indole 3 Acetic Acid

Potensi Bakteri Endofit Ubi Jalar (Ipomoea batatas L.) dalam Menghasilkan Hormon Indole Acetic Acid (IAA) dengan Penambahan L-triptofan(Potential of endophytic bacteria of sweet potato (Ipomoea batatas L.) in producing Indole Acetic Acid (IAA) with the addition of L-tryptophan) Agustina Monalisa TangapoProgram Studi Biologi FMIPA Universitas Sam RatulangiJl. Kampus Unsrat, Manado 95115*Email korespondensi:[email protected] (Article History: Received 5-01-2019; Revised 15-01-2020; Accepted 05-02-2020) ABSTRAKAsosiasi bakteri-tanaman, dapat mempengaruhi produktivitas tanaman secara langsung dan tidak langsung. Secara langsung, salah satunya yaitu bakteri dapat memproduksi dan menyekresikan zat pengatur tumbuh indole-3-acetic acid (IAA, auksin). Penelitian ini bertujuan untuk menguji kemampuan bakteri endofit ubi jalar dalam menghasilkan IAA. Metode analisis IAA dilakukan dengan metode kolorimetri. Analisis produksi IAA dilakukan dengan penambahan dan tanpa penambahan L-triptofan. Hasil penelitian menunjukkan bahwa tanpa penambahan L-triptofan, diperoleh sejumlah 19 jenis yang menghasilkan IAA dengan kisaran konsentrasi 0,29-7,21 mg/L. Dengan penambahan L-triptofan, jumlah jenis positif dan konsentrasi IAA yang dihasilkan meningkat signifikan. Jumlah jenis positif 20 jenis (91%) dan konsentrasi IAA yang dihasilkan mencapai kisaran 0,96-115,63 mg/L.Kata kunci: bakteri endofit; IAA; ubi jalar; L-triptofan ABSTRACTPlant-bacteria associations, can promote plant growth by both direct and indirect mechanisms. One of direct mechanisms is that bacteria can produce and secrete indole-3-acetic acid (IAA, auxin) growth regulators. This study aims to examine the ability of sweet potato endophytic bacteria to produce IAA. The detection of IAA production was conducted by colorimetric technique. IAA production analysis was carried out with addition and without addition of L-tryptophan. Without the addition of L-tryptophan, a total of 19 species produced IAA with a concentration range of 0.29-7.21 mg/L. With the addition of L-tryptophan, the number of positive species and the concentration of IAA produced increased significantly. The number of positive species was 20 species (91%) and the concentration of IAA produced reached a range of 0.96-115.63 mg/L.Keywords: endophytes bacterial; IAA; sweet potato; L-tryptophan

Sign in / Sign up

Export Citation Format

Share Document