A new continuous-flow solar water disinfection system inactivating cysts of Acanthamoeba castellanii, and bacteria

SUMMARYSpecies belonging to the generaCryptosporidiumare recognized as waterborne pathogens. Solar water disinfection (SODIS) is a simple method that involves the use of solar radiation to destroy pathogenic microorganisms that cause waterborne diseases. A notable increase in water temperature and the existence of a large number of empty or partially excysted (i.e. unviable) oocysts have been observed in previous SODIS studies with water experimentally contaminated withCryptosporidium parvumoocysts under field conditions. The aim of the present study was to evaluate the effect of the temperatures that can be reached during exposure of water samples to natural sunlight (37–50°C), on the excystation ofC. parvumin the absence of other stimuli. In samples exposed to 40–48°C, a gradual increase in the percentage of excystation was observed as the time of exposure increased and a maximum of 53·81% of excystation was obtained on exposure of the water to a temperature of 46°C for 12 h (versus8·80% initial isolate). Under such conditions, the oocyst infectivity evaluated in a neonatal murine model decreased statistically with respect to the initial isolate (19·38%versus100%). The results demonstrate the important effect of the temperature on the excystation ofC. parvumand therefore on its viability and infectivity.

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In vitro toxicity studies of novel solar water disinfection reactors using the E-screen bioassay and the Ames test

H2Open Journal ◽

10.2166/h2oj.2021.108 ◽

2021 ◽

Author(s):

Paloma Ozores Diez ◽

M. Inmaculada Polo-López ◽

Azahara Martínez-García ◽

Monique Waso ◽

Brandon Reyneke ◽

...

Keyword(s):

Ames Test ◽

Daily Intake ◽

Cost Effective ◽

Water Disinfection ◽

In Vitro Toxicity ◽

Toxic Substances ◽

Solar Water ◽

Point Of Use ◽

Solar Water Disinfection

Abstract Solar water disinfection (SODIS) is a cost-effective point of use method for disinfecting water, usually in a 2 L polyethylene terephthalate (PET) plastic bottle. To increase the volume of water disinfected, three novel transparent reactors were developed using PET in 25 L transparent jerrycans, polymethyl methacrylate (PMMA) in tubular solar reactors capable of delivering >20 L of water and polypropylene (PP) in 20 L buckets. In vitro bioassays were used to investigate any toxic substances leached from the plastic reactors into disinfected water as a result of exposure to sunshine for up to 9 months. The Ames test was used to test for mutagenicity and the E-screen bioassay to test for estrogenicity. No mutagenicity was detected in any sample and no estrogenicity was found in the SODIS treated water produced by the PMMA reactors or the PP buckets. While water disinfected using the PET reactors showed no estrogenicity following exposure to the sun for 3 and 6 months, estrogenicity was detected following 9 months' exposure to sunlight; however levels detected were within the acceptable daily intake for 17β-estradiol (E2) of up to 50 ng/kg body weight/day.

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