Chemiluminescence determination of catecholamines in human blood plasma and urine using 1,2-diphenylethylenediamine as pre-column derivatization reagent in liquid chromatography

1994 ◽  
Vol 298 (3) ◽  
pp. 431-438 ◽  
Author(s):  
Gamal H. Ragab ◽  
Hitoshi Nohta ◽  
Masaaki Kai ◽  
Yosuke Ohkura
Keyword(s):  
Liquid Chromatography ◽  
Blood Plasma ◽  
Human Blood ◽  
Human Blood Plasma

scholarly journals Development and validation of an improved HPLC-UV method for simultaneous determination of lamotrigine and oxcarbazepine and its active metabolite 10,11-dihydro-10-hydroxycarbazepine in human blood plasma and comparison with an UHPLC-MS/MS method

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Siyao Jin ◽  
Qing Zhao ◽  
Dongjie Zhang ◽  
Zhigang Zhao ◽  
Shenghui Mei
Keyword(s):  
Liquid Chromatography ◽  
Blood Plasma ◽  
Simultaneous Determination ◽  
Human Blood ◽  
High Performance ◽  
Internal Standard ◽  
Ultra Performance Liquid Chromatography ◽  
Human Blood Plasma ◽  
Elution Time

AbstractLamotrigine (LTG) and oxcarbazepine (OXC) are first-line drugs for epilepsy treatment. Their large pharmacokinetics variabilities and relations between efficacy and toxicity and blood plasma concentration require routine monitoring for dose adjustment. In this study, we developed and validated a simple, accurate, and reliable method for simultaneous determination of LTG, OXC and 10,11-dihydro-10-hydroxycarbazepine (MHD) in human blood plasma by high-performance liquid chromatography-ultraviolet detection (HPLC-UV) with a simple one-step protein precipitation using methanol (1% acetic acid) and 15 min elution time under isocratic elution at 1 mL/min. Calibration range was 2.4 to 120 mg/L for LTG, OXC, and MHD. The intra-day and inter-day bias were − 8.84 to 4.18%, and the imprecision was less than 8.08% for all analytes. The internal standard (fluconazole) normalized recovery was 96.30 to 107.69% for LTG, 98.51 to 111.04% for MHD, and 95.04 to 109.86% for OXC. A total of 186 LTG samples and 25 MHD samples were used to evaluate the agreement between HPLC-UV and ultra-performance liquid chromatography-mass spectrometry (UHPLC-MS/MS) by Passing-Bablok regression and Bland-Altman plot. The mean bias and the 95% limits of agreement (95% LOA) of the two measurements were 0.575 mg/L and − 1.238 to 2.387 mg/L for LTG (n = 186) and − 1.222 mg/L and − 8.271 to 5.827 mg/L for MHD (n = 25), which indicated the UV method was comparable with the MS method for LTG and MHD analysis.

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