Fluorometric continuous kinetic assay of α-chymotrypsin using new protease substrates possessing long-wave excitation and emission maxima

1988 ◽  
Vol 171 (2) ◽  
pp. 393-397 ◽  
Author(s):  
John H. Baustert ◽  
Otto S. Wolfbeis ◽  
Robert Moser ◽  
Ernst Koller
2015 ◽  
Vol 3 (5) ◽  
pp. 3057-3075 ◽  
Author(s):  
T. K. Papathanasiou ◽  
A. E. Karperaki ◽  
E. E. Theotokoglou ◽  
K. A. Belibassakis

Abstract. The transient hydroelastic response of an ice shelf under long wave excitation is analysed by means of the finite element method. The simple model, presented in this work, is used for the simulation of the generated kinematic and stress fields in an ice shelf, when the latter interacts with a tsunami wave. The ice shelf, being of large length compared to its thickness, is modelled as an elastic Euler–Bernoulli beam, constrained at the grounding line. The hydrodynamic field is represented by the linearised shallow water equations. The numerical solution is based on the development of a special hydroelastic finite element for the system of governing of equations. Motivated by the 2011 Sulzberger Ice Shelf (SIS) calving event and its correlation with the Honshu Tsunami, the SIS stable configuration is studied. The extreme values of the bending moment distribution in both space and time are examined. Finally, the location of these extrema is investigated for different values of ice shelf thickness and tsunami wave length.


2015 ◽  
Vol 15 (8) ◽  
pp. 1851-1857 ◽  
Author(s):  
T. K. Papathanasiou ◽  
A. E. Karperaki ◽  
E. E. Theotokoglou ◽  
K. A. Belibassakis

Abstract. The transient hydroelastic response of an ice shelf under long wave excitation is analysed by means of the finite element method. The simple model, presented in this work, is used for the simulation of the generated kinematic and stress fields in an ice shelf, when the latter interacts with a tsunami wave. The ice shelf, being of large length compared to its thickness, is modelled as an elastic Euler-Bernoulli beam, constrained at the grounding line. The hydrodynamic field is represented by the linearised shallow water equations. The numerical solution is based on the development of a special hydroelastic finite element for the system of governing of equations. Motivated by the 2011 Sulzberger Ice Shelf (SIS) calving event and its correlation with the Honshu Tsunami, the SIS stable configuration is studied. The extreme values of the bending moment distribution in both space and time are examined. Finally, the location of these extrema is investigated for different values of ice shelf thickness and tsunami wave length.


1985 ◽  
Vol 50 (6) ◽  
pp. 1312-1328 ◽  
Author(s):  
Jan Kovář ◽  
Karel Šimek ◽  
Eva Kožoušková ◽  
Hana Klukanová ◽  
Jiří Slavík

The fluorescence properties of 28 isoquinoline alkaloids have been investigated. In most of them the chromophore responsible for fluorescence was the benzene ring with electron-donor substituents containing oxygen. The long-wave excitation peak practically coincides with the long-wave absorption peak of these substances, covering the region from 284 to 293 nm, the maximum emission being in the range 320-332 nm. With alkaloids having a number of conjugated rings, both excitation and emission were observed at higher wavelengths. Only protoberberine alkaloids behaved as hydrophobic probes, i.e. transfer of these compounds into a less polar medium produced a marked hypsochromic shift and a higher intensity of emission. The effect of polarity on the behaviour of tetrahydroprotoberberines, protopines, pavinanes, aporphines and benzophenanthridines was not so pronounced. Changes of pH manifested themselves most markedly in compounds with dissociable hydroxyl groups; the majority of phenolates did not fluoresce.The phenol group pK values of these compounds in the excited state were lower than in the ground state (which ranged between 8.4 and 10.4). The relations between the apparent pK, determined from fluorescence data, and the pK's of these compounds in the ground and the excited states are discussed.


1986 ◽  
Vol 1 (20) ◽  
pp. 147 ◽  
Author(s):  
J.K. Kostense ◽  
K.L. Meijer ◽  
M.W. Dingemans ◽  
A.E. Mynett ◽  
P. Van den Bosch

A finite element model for combined refraction-diffraction problems of linear water waves has been extended to include the effect of various dissipative mechanisms on wave excitation response in harbours of arbitrary shape and variable depth. Especially, the effects of bottom friction, partial absorption along the harbour, contours, and transmission through permeable breakwaters have been considered. Although, within the mild slope approximation, the model is valid for arbitrary wave lengths, in this paper its effectiveness for harbour design applications is demonstrated for long wave induced resonance phenomena. For this purpose a realistic harbour geometry has been selected. A hydraulic scale-model of this harbour enabled experimental verification of the computational results.


1962 ◽  
Vol 4 (1) ◽  
pp. 97-104 ◽  
Author(s):  
H. Stammreich

2001 ◽  
Vol 11 (PR2) ◽  
pp. Pr2-285-Pr2-288
Author(s):  
R. Tommasini ◽  
E. E. Fill

2009 ◽  
pp. 26-38 ◽  
Author(s):  
S. Glaziev

The article analyzes fundamental reasons for the world economic crisis in the light of global technological shifts. It proves that it is caused by the substitution of technological modes. It is shown that sharp increase and slump in stock indices and prices for energy resources are typical of the process of technological substitution which occurs regularly according to the rhythm of long-wave fluctuations of the world economic activity. The article rationalizes a package of anti-crisis measures aimed at stimulating the new technological mode. Its structure and role of the locomotive factor of the new long wave of economic growth are revealed.


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