Protective effect of the plasticizer di(2-ethylhexyl) phthalate against damage of the mitochondrial membrane induced by calcium: possible participation of the adenine nucleotide translocator

1989 ◽  
Vol 985 (3) ◽  
pp. 286-292 ◽  
Author(s):  
Shinichi Kora ◽  
Mineo Sado ◽  
Haruhiko Koike ◽  
Hiroshi Terada
Keyword(s):  
Protective Effect ◽  
Mitochondrial Membrane ◽  
Adenine Nucleotide ◽  
Adenine Nucleotide Translocator ◽  
Ethylhexyl Phthalate

scholarly journals Control of Mitochondrial Membrane Permeabilization by Adenine Nucleotide Translocator Interacting with HIV-1 Viral Protein R and Bcl-2

2001 ◽  
Vol 193 (4) ◽  
pp. 509-520 ◽  
Author(s):  
Etienne Jacotot ◽  
Karine F. Ferri ◽  
Chahrazed El Hamel ◽  
Catherine Brenner ◽  
Sabine Druillennec ◽  
...  
Keyword(s):  
Viral Protein ◽  
Mitochondrial Membrane ◽  
Adenine Nucleotide ◽  
Specific Interaction ◽  
Membrane Permeabilization ◽  
Adenine Nucleotide Translocator ◽  
Channel Formation ◽  
Viral Protein R ◽  
Mitochondrial Membrane Permeabilization ◽  
Hiv 1

Viral protein R (Vpr), an apoptogenic accessory protein encoded by HIV-1, induces mitochondrial membrane permeabilization (MMP) via a specific interaction with the permeability transition pore complex, which comprises the voltage-dependent anion channel (VDAC) in the outer membrane (OM) and the adenine nucleotide translocator (ANT) in the inner membrane. Here, we demonstrate that a synthetic Vpr-derived peptide (Vpr52-96) specifically binds to the intermembrane face of the ANT with an affinity in the nanomolar range. Taking advantage of this specific interaction, we determined the role of ANT in the control of MMP. In planar lipid bilayers, Vpr52-96 and purified ANT cooperatively form large conductance channels. This cooperative channel formation relies on a direct protein–protein interaction since it is abolished by the addition of a peptide corresponding to the Vpr binding site of ANT. When added to isolated mitochondria, Vpr52-96 uncouples the respiratory chain and induces a rapid inner MMP to protons and NADH. This inner MMP precedes outer MMP to cytochrome c. Vpr52-96–induced matrix swelling and inner MMP both are prevented by preincubation of purified mitochondria with recombinant Bcl-2 protein. In contrast to König's polyanion (PA10), a specific inhibitor of the VDAC, Bcl-2 fails to prevent Vpr52-96 from crossing the mitochondrial OM. Rather, Bcl-2 reduces the ANT–Vpr interaction, as determined by affinity purification and plasmon resonance studies. Concomitantly, Bcl-2 suppresses channel formation by the ANT–Vpr complex in synthetic membranes. In conclusion, both Vpr and Bcl-2 modulate MMP through a direct interaction with ANT.

scholarly journals Adenine nucleotide translocator mediates the mitochondrial membrane permeabilization induced by lonidamine, arsenite and CD437

Oncogene ◽  
2001 ◽  
Vol 20 (52) ◽  
pp. 7579-7587 ◽  
Author(s):  
Anne-Sophie Belzacq ◽  
Chahrazed El Hamel ◽  
Helena L A Vieira ◽  
Isabel Cohen ◽  
Delphine Haouzi ◽  
...  
Keyword(s):  
Mitochondrial Membrane ◽  
Adenine Nucleotide ◽  
Membrane Permeabilization ◽  
Adenine Nucleotide Translocator ◽  
Mitochondrial Membrane Permeabilization

Protective effect of D-psicose against testicular atrophy induced by di(2-ethylhexyl) phthalate

2013 ◽  
Vol 70 (4) ◽  
pp. 560-565 ◽  
Author(s):  
Shigeru Suna ◽  
Masaaki Tokuda ◽  
Tomohiro Hirao ◽  
Fuminori Yamaguchi ◽  
Nobuyuki Miyatake ◽  
...  
Keyword(s):  
Protective Effect ◽  
Testicular Atrophy ◽  
Ethylhexyl Phthalate
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