Effect of various short and long chain fatty acids on the dextran sulfate-low density lipoprotein interaction

1969 ◽  
Vol 187 (3) ◽  
pp. 444-446 ◽  
Author(s):  
Uri Cogan ◽  
Toshiro Nishida
Keyword(s):  
Fatty Acids ◽  
Dextran Sulfate ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Long Chain Fatty Acids ◽  
Long Chain ◽  
Chain Fatty Acids

Insufficient accuracy and specificity of polyanion precipitation methods for quantifying low-density lipoproteins

1990 ◽  
Vol 36 (12) ◽  
pp. 2109-2113 ◽  
Author(s):  
R Siekmeier ◽  
W März ◽  
W Gross
Keyword(s):  
Fatty Acids ◽  
Dextran Sulfate ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density Lipoproteins ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Nonesterified Fatty Acids ◽  
High Concentrations

Abstract Recently, polyanion precipitation assays for low-density lipoprotein (LDL)-cholesterol have been found to underestimate their analyte in normolipidemic samples (Siekmeier et al., Clin Chim Acta 1988;177:221-30). Therefore, accuracy, specificity, and interference by nonesterified fatty acids have been studied for three precipitants (obtained by heparin, dextran sulfate, or polyvinyl sulfate precipitation). At normal concentrations of LDL, precipitation is incomplete, whereas it is nearly quantitative at high concentrations of LDL. The polyvinyl sulfate reagent markedly responds to variations in the amount of non-LDL protein present in the precipitation mixture. In the dextran sulfate and the polyvinyl sulfate method, but not in the heparin method, the percentages of LDL precipitated notably increase as the concentration of the polyanion compound is decreased. In either assay, very-low-density lipoproteins, but not high-density lipoproteins, are significantly coprecipitated (dextran sulfate 28%, polyvinyl sulfate and heparin 66%) in a concentration-independent fashion. Increased concentrations of nonesterified fatty acids markedly interfere with the dextran sulfate and polyvinyl sulfate assay, but do not much affect results with the heparin reagent.

Unsaturated long-chain fatty acids inhibit the binding of oxidized low-density lipoproteins to a model CD36

2014 ◽  
Vol 78 (2) ◽  
pp. 238-244 ◽  
Author(s):  
Marie Takai ◽  
Yuki Kozai ◽  
Satoshi Tsuzuki ◽  
Yukari Matsuno ◽  
Maiko Fujioka ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Low Density Lipoproteins ◽  
Low Density ◽  
Long Chain Fatty Acids ◽  
Oxidized Low Density Lipoproteins ◽  
Long Chain ◽  
Chain Fatty Acids
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