Scanning electron microscopy of myogenesis in monolayer culture: A preliminary study

1972 ◽  
Vol 29 (2) ◽  
pp. 227-233 ◽  
Author(s):  
Yutaka Shimada
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Monolayer Culture ◽  
Preliminary Study ◽  
Scanning Electron

Asbestos fiber identification in liver from cholangiocarcinoma patients living in an asbestos polluted area: a preliminary study

2019 ◽  
Vol 105 (5) ◽  
pp. 404-410 ◽  
Author(s):  
Federica Grosso ◽  
Alessandro Croce ◽  
Roberta Libener ◽  
Narciso Mariani ◽  
Massimo Pastormerlo ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Asbestos Exposure ◽  
Optical Microscope ◽  
Variable Pressure ◽  
Chrysotile Asbestos ◽  
Neoplastic Tissue ◽  
Asbestos Fibers ◽  
Preliminary Study ◽  
Scanning Electron

Purpose: To assess whether asbestos fibers may be observed in liver tissue of patients with cholangiocarcinoma (CC) with environmental or working asbestos exposure. Methods: Detection of fibers was performed directly on histologic sections of liver from 7 patients with CC using optical microscope and variable pressure scanning electron microscopy equipped with energy-dispersive spectroscopy (VP-SEM/EDS). All patients were from Casale Monferrato, Italy, a highly asbestos-polluted town. Due to ethical constraints, observers were blinded to patients’ clinical features. Results: Fibers/bundles of fibers of chrysotile were detected in 5 out of 7 patients (71%). The boundary between healthy and neoplastic tissue or the fibrocollagen tissue produced by the neoplasia were identified as areas of fiber incorporation. Conclusions: This study is the first report about the detection of chrysotile asbestos fibers in the liver of patients with CC. Further studies on larger cohorts are needed to corroborate our preliminary findings.

A Preliminary Study on the Anti-Mosquito Nanocapsule

2014 ◽  
Vol 487 ◽  
pp. 98-101 ◽  
Author(s):  
Jun Hua Wang ◽  
Gang Huang ◽  
Yong Tang Jia
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Particle Size ◽  
Emulsion Polymerization ◽  
Ethyl Acrylate ◽  
Particle Size Analyzer ◽  
Micro Emulsion ◽  
The Mean ◽  
Preliminary Study ◽  
Scanning Electron

Anti-mosquito nanocapsules were prepared using the copolymer of styrene (ST) and ethyl acrylate (EA) as wall and deet as core by micro-emulsion polymerization. The mean particle size of nanocapsules was about 424nm and the loading of deet was about 73% under the conditions of 2% SDS as emulsifier and the ratio of core/wall 1:1. Scanning electron microscopy and laser particle size analyzer were employed to characterize the nanocapsules.

scholarly journals Physical Characterisation of a Natural Zeolite using a Scanning Electron Microscopy: A Preliminary Study

2021 ◽  
Vol 1 (1) ◽  
pp. 510-513
Author(s):  
Ali Munawar ◽  
Djoko Mulyanto ◽  
R. R. Dina Asrifah
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Natural Zeolite ◽  
Mine Drainage ◽  
The Other ◽  
Muffle Furnace ◽  
Dissolved Metals ◽  
Metallic Mineral ◽  
Preliminary Study ◽  
Scanning Electron

Zeolite is one of important non-metallic mineral deposites in Indonesia. It has been used for various purposes, including as an adsobent in environmental protection, industry, and agriculture. The most important characteristics of zeolit to be an adsorbent is its surface area and crystal structure. To optimally use zeolite as an adsorbent, it is necessary to understand its physical characteristics. This preliminary study was aimed to characterize physical properties of a natural zeolite obtained from Tasikmalaya, West Java, Indonesia. The zeolite material was crushed into <0.5 mm diameter and divided into two sets of samples. One set of samples was heated in a muffle furnace at 250 oC for two hours and the other set was left untreated. All samples were then observed using a Scanning Electron Microscopy (SEM). The SEM micrographs showed rough and porous structure and defined crystallinity of the zeolite. Thermal treatment at 250 oC increased zeolite crystallinity. These results confirm that this natural zeolite is potential to be used as an adsorbent to remove dissolved metals from acid mine drainage.

Growth of DLD-1 Colon Cancer Cells on Variotis™ Scaffolds of Controlled Porosity: A Preliminary Study

2010 ◽  
Vol 8 ◽  
pp. 79-89 ◽  
Author(s):  
Yu Jia Ma ◽  
Nicole S. Bryce ◽  
Renee M. Whan ◽  
Lucy Xiao ◽  
Kai Li ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Colon Cancer ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
3D Scaffolds ◽  
Interconnected Porosity ◽  
Preliminary Study ◽  
Hoechst Staining ◽  
Scanning Electron

Tissue engineering will play an increasingly vital role in cancer research. Provision of biomimetic microenvironment systems for in vitro cancer models can be addressed in part by utilizing thick 3D scaffolds with high interconnective porosity . This approach gives rise to new analytical challenges and opportunities. In this preliminary study, Variotis™ synthetic scaffolds of high interconnected porosity and hierarchical structure were used. An effective macroscopic porosity of 94.3 ±1.74 vol% was attained by using microCT and finite element methods. The actual porosity was determined to be 94.6±0.29 vol%. Scaffolds were compressed in a customized jig to thicknesses of 99.5 mm, 74.6 mm, 46.3 mm (±0.5% tolerance) and then annealed to set respective porosities of 94.3 vol%, 93.2 vol%, 89.5 vol% (±1.5% tolerance). Scaffolds were then sectioned to 2mm thickness. DLD-1 colon cancer cells were grown on 3D scaffolds of three specified porosities for varying periods of time then imaged using confocal and scanning electron microscopy methods. Hoechst staining resulted with minimal scaffold autofluoresence while autofluoresence exceeded useful limits when used in conjunction with Alexa488-phalloidin under argon laser excitation in confocal microscopy. Using Hoechst staining, DLD-1 cells (nuclei) were observed to readily attach and proliferate on Variotis™ scaffolds. Normal DLD-1 cell morphologies were evident using scanning electron microscopy. The high interconnected porosity of the scaffolds allowed cells to be observed deep within scaffolds. Scaffolds remained structurally stable and unified throughout all culture experiments and provided ease of handling during cell culture and microscopy.

scholarly journals Preliminary Study on Scanning Electron Microscopy as an Additional Mean to Assess the Effectiveness of Sterilization of Endoscopes Used in Digestive Tract Examination

2020 ◽  
Vol 71 (2) ◽  
pp. 280-287
Author(s):  
Delia Eugenia Bercean (Vitan) ◽  
Rahela Carpa ◽  
Doina Prodan ◽  
Marioara Moldovan ◽  
Cristina Borzan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Gastrointestinal Endoscopy ◽  
Sem Analysis ◽  
Sterilization Method ◽  
Preliminary Study ◽  
High Level ◽  
Microbiological Analyses ◽  
Scanning Electron

Microbiological analyses are performed to check the quality of reprocessing procedures and represent a regular instrument of quality control in gastrointestinal endoscopy. They help detecting and redressing the possible flaws of the reprocessing procedure, thus preventing the transmission of germs through endoscopy. In this paper the effectiveness of sterilization is characterized and compared by scanning electron microscopy and by classical microbiological analyses, for three types of medical devices, with two sterilization methods. The high level manual sterilization method is superior to the automatic reprocessing sterilization method. The sterilization time is important because after changing the immersion in disinfectant period, from 15 minutes to 20 minutes, no more microbial forms were detected, neither at microbiological examination nor at SEM analysis.

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