A Preliminary Study on the Peritubular Structure of Humau Dentinal Tubules by Scanning Electron Microscopy

This in vitro study evaluated the effects of dentifrices containing nano-hydroxyapatite (n-HAp) on dentinal tubule occlusion and on mineral deposition. Dentin specimens of ten human teeth were submersed for 30 s in 40% citric acid and then randomly divided into four groups (three study groups and one control group). In the study groups, the dentin samples were exposed to three different n-HAp toothpastes: Karex (Dr. Kurt Wolff GmbH & Co. KG, Bielefeld, Germany), Biorepair Plus Sensitive (Coswell SpA, Bologna, Italy), and Dr. Wolff’s Biorepair (Dr. Kurt Wolff GmbH & Co. KG, Bielefeld, Germany); in the control group no toothpaste was applied. All of the samples were evaluated using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) analysis. In the control group all of the samples showed a frank and wide opening of the dentinal tubules, whereas in the study groups different degrees of tubule closure by mineral depositions were observed. Toothpastes containing n-HAp determined a significant occlusion of dentinal tubules and a significant increase of mineral deposition on the dentin surface. All three tested toothpastes showed similar results regarding the degree of dentinal tubule closure. Varying degrees of differences in calcium, phosphate, carbon, and oxygen ion concentrations among the three tested toothpastes were obtained.

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Asbestos fiber identification in liver from cholangiocarcinoma patients living in an asbestos polluted area: a preliminary study

Tumori Journal ◽

10.1177/0300891619839305 ◽

2019 ◽

Vol 105 (5) ◽

pp. 404-410 ◽

Cited By ~ 2

Author(s):

Federica Grosso ◽

Alessandro Croce ◽

Roberta Libener ◽

Narciso Mariani ◽

Massimo Pastormerlo ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Asbestos Exposure ◽

Optical Microscope ◽

Variable Pressure ◽

Chrysotile Asbestos ◽

Neoplastic Tissue ◽

Asbestos Fibers ◽

Preliminary Study ◽

Scanning Electron

Purpose: To assess whether asbestos fibers may be observed in liver tissue of patients with cholangiocarcinoma (CC) with environmental or working asbestos exposure. Methods: Detection of fibers was performed directly on histologic sections of liver from 7 patients with CC using optical microscope and variable pressure scanning electron microscopy equipped with energy-dispersive spectroscopy (VP-SEM/EDS). All patients were from Casale Monferrato, Italy, a highly asbestos-polluted town. Due to ethical constraints, observers were blinded to patients’ clinical features. Results: Fibers/bundles of fibers of chrysotile were detected in 5 out of 7 patients (71%). The boundary between healthy and neoplastic tissue or the fibrocollagen tissue produced by the neoplasia were identified as areas of fiber incorporation. Conclusions: This study is the first report about the detection of chrysotile asbestos fibers in the liver of patients with CC. Further studies on larger cohorts are needed to corroborate our preliminary findings.

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Penetration of Different Impression Materials into Exposed Dentinal Tubules during the Impression Procedure

Materials ◽

10.3390/ma13061321 ◽

2020 ◽

Vol 13 (6) ◽

pp. 1321 ◽

Cited By ~ 2

Author(s):

Bruna Sinjari ◽

Gianmaria D’Addazio ◽

Edit Xhajanka ◽

Sergio Caputi ◽

Giuseppe Varvara ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Fractal Dimensions ◽

Excellent Performance ◽

Dentinal Tubules ◽

Impression Materials ◽

Electron Microscopy Analysis ◽

Microscopy Analysis ◽

The Mean ◽

Scanning Electron

Adhesive restorations have been shown to guarantee excellent performance and longevity, although this comes with some disadvantages. Among these, the vulnerability of dentine to different agents has been widely evaluated. The aim of this study was to evaluate the possible penetration of impression materials into freshly cut dentine. Dentine from 27 teeth was impressed with polyether (Impregum Penta L) (nine teeth) and with polyvinyl siloxane (Aquasil Ultra LV) (nine teeth). The surface of nine teeth after the impressions were used as the control. Specifically, the extroflections caused by the imprinting of the dentinal tubules on the impression material, the so-called impression tags, were measured. Furthermore, the presence of the material inside the tubules was examined. Scanning electron microscopy analysis showed material tags for all of the experimental groups. The mean lengths (±SD) were 22.6 (±11.0) µm for polyether, 21.8 (±12.8) µm for polyvinyl siloxane and 11.3 (±7.0) µm for the tooth control, with mean diameters (±SD) of 2.8 (±0.5), 2.4 (±0.7) and 3.1 (±0.7) µm, respectively. Fractal analysis showed fractal dimensions of 1.78 (±0.03), 1.77 (±0.03) and 1.71 (±0.03), respectively. These data demonstrated that the impression materials can remain inside the dentinal tubules, which can adversely affect the adhesive procedures.

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Evaluation of Residual Dentin after Conventional and Chemomechanical Caries Removal Using SEM

Journal of Clinical Pediatric Dentistry ◽

10.17796/jcpd.32.2.44n2787118133880 ◽

2007 ◽

Vol 32 (2) ◽

pp. 115-120 ◽

Cited By ~ 11

Author(s):

Fernanda Nahás Pires Corrêa ◽

Leonardo Eloy Rodrigues Filho ◽

Célia Regina Martins Delgado Rodrigues

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

In Vitro Study ◽

Adhesive System ◽

Smear Layer ◽

Dentinal Tubules ◽

Removal Method ◽

Caries Removal ◽

Scanning Electron

The purpose of this in vitro study was to analyze the residual dentinal surfaces following caries removal using rotatory instruments and two chemomechanical methods (Papacárie® and Carisolv®), by scanning electron microscopy (SEM). Thirty primary incisors were divided into three groups, according to the caries removal method used, and their residual dentin was examined under SEM (15). After caries removal, 15 of these teeth were restored with Single Bond (3M) adhesive system and Z100 Filtek composite resin (3M). The tags of the replicas were observed under SEM. The chemomechanical caries removal methods (Papacárie®and Carisolv®) formed an amorphous layer, similar to the smear layer and few exposed dentinal tubules;the conventional caries removal method produced a smooth and regular dentinal surface, with typical smear layer and exposed dentinal tubules. All groups showed abundant tag formation. Scanning electron microscopy analysis revealed a difference between dentin treated with rotatory instruments and that treated with chemomechanical methods in spite of the occurrence of a similar tag formation in both groups.

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Residual Filling Material in Dentinal Tubules after Gutta-percha Removal Observed with Scanning Electron Microscopy

Journal of Endodontics ◽

10.1016/j.joen.2011.11.016 ◽

2012 ◽

Vol 38 (3) ◽

pp. 293-296 ◽

Cited By ~ 10

Author(s):

Li-li Xu ◽

Lan Zhang ◽

Xue-dong Zhou ◽

Rui Wang ◽

Yan-han Deng ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Filling Material ◽

Dentinal Tubules ◽

Gutta Percha ◽

Scanning Electron

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A quantitative and diametral analysis of human dentinal tubules at pulp chamber ceiling and floor under scanning electron microscopy

Australian Endodontic Journal ◽

10.1111/aej.12068 ◽

2014 ◽

Vol 41 (1) ◽

pp. 29-34 ◽

Cited By ~ 3

Author(s):

Evangelos G. Kontakiotis ◽

Ioannis N. Tsatsoulis ◽

Christos G. Filippatos ◽

Anastasia Agrafioti

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Pulp Chamber ◽

Dentinal Tubules ◽

Scanning Electron

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Structure and microstructure of coronary dentin in non-erupted human deciduous incisor teeth

Brazilian Dental Journal ◽

10.1590/s0103-64402002000300005 ◽

2002 ◽

Vol 13 (3) ◽

pp. 170-174 ◽

Cited By ~ 2

Author(s):

Luciane R.R S. Costa ◽

Ii-Sei Watanabe ◽

Márcia C. Kronka ◽

Marcelo C.P. Silva

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Nitric Acid ◽

Light Microscopy ◽

Three Dimensional ◽

Dentinal Tubules ◽

Sem Study ◽

Structure And Microstructure ◽

Hematoxylin Eosin ◽

Scanning Electron

The dentin structure of non-erupted human deciduous mandibular and maxillary central and lateral incisor teeth was studied employing light and scanning electron microscopy. For light microscopy, nitric-acid-demineralized and ground sections were used. The sections were stained by hematoxylin-eosin, picrosirius and azo-carmim methods, and ground specimens were prepared using a carborundum disk mounted in a handpiece. For SEM study, teeth were frozen in liquid nitrogen and fractured at longitudinal and transversal directions. Structurally, demineralization and ground methods revealed tubules with primary and secondary curvatures, canaliculi, giant tubules, interglobular dentin, predentin, and intertubular dentin. Scanning electron microscopy showed three-dimensional aspects of dentinal tubules, canaliculi, peritubular dentin, intertubular dentin, and predentin. This study contributes to knowledge about dentin morphology showing characteristics of teeth not yet submitted to mastication stress.

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