Muscarinic M1 receptor agonist actions of muscarinic receptor agonists in rabbit vas deferens

1993 ◽  
Vol 232 (1) ◽  
pp. 47-57 ◽  
Author(s):  
Harlan E. Shannon ◽  
Barry D. Sawyer ◽  
Kerry G. Bemis ◽  
Frank P. Bymaster ◽  
Ian Heath ◽  
...  
Keyword(s):  
Muscarinic Receptor ◽  
Receptor Agonist ◽  
Vas Deferens ◽  
Receptor Agonists ◽  
M1 Receptor ◽  
Rabbit Vas Deferens

Rabbit vas deferens: A specific bioassay for opioid κ-receptor agonists

1981 ◽  
Vol 73 (2-3) ◽  
pp. 235-236 ◽  
Author(s):  
Tetsuo Oka ◽  
Kazuko Negishi ◽  
Mitsuaki Suda ◽  
Teruhiko Matsumiya ◽  
Toshiyuki Inazu ◽  
...  
Keyword(s):  
Vas Deferens ◽  
Receptor Agonists ◽  
Rabbit Vas Deferens ◽  
Κ Receptor

scholarly journals Protein kinase C is involved in desensitization of muscarinic receptors induced by phorbol esters but not by receptor agonists

1990 ◽  
Vol 267 (1) ◽  
pp. 23-29 ◽  
Author(s):  
W S Lai ◽  
T B Rogers ◽  
E E el-Fakahany
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Cell Surface ◽  
Muscarinic Receptor ◽  
Muscarinic Receptors ◽  
Receptor Agonist ◽  
Phorbol Esters ◽  
Down Regulation ◽  
Receptor Agonists ◽  
Kinase C

Preincubation with receptor agonists or phorbol esters desensitized muscarinic-receptor-mediated [3H]cyclic GMP responses in mouse neuroblastoma N1E-115 cells. However, desensitization mediated by phorbol esters was heterologous, whereas that effected by receptor agonist was specific towards the muscarinic receptors. In addition, there was no loss of cell surface muscarinic receptors, as measured by the binding of the hydrophilic ligand [3H]N-methylscopolamine, when cells were treated with phorbol esters, but receptor-agonist-induced desensitization was accompanied by a decrease in cell surface receptor density. We examined the role of protein kinase C (PKC) in the desensitization of muscarinic receptors by employing a kinase inhibitor and by down-regulation of PKC by long-term incubation of cells with phorbol esters. Whereas these manoeuvres had marked effects on phorbol-ester-induced desensitization of muscarinic responses, they did not block agonist-induced down-regulation and desensitization of muscarinic receptors. In addition, when phosphoinositide hydrolysis was suppressed, the muscarinic agonist was still capable of mediating receptor sequestration and desensitization. These results suggest that the mechanisms for regulating muscarinic receptor sensitivity could be both PKC-dependent and PKC-independent, being mediated by phorbol esters and receptor agonists respectively.

Differences between rat dorsal and ventral hippocampus in muscarinic receptor agonist binding and interaction with phospholipase C

1993 ◽  
Vol 244 (2) ◽  
pp. 125-131 ◽  
Author(s):  
Antonio J. Garcia Ruiz ◽  
Matilde Zambelli ◽  
Caterina La Porta ◽  
Herbert Ladinsky ◽  
Silvana Consolo
Keyword(s):  
Phospholipase C ◽  
Muscarinic Receptor ◽  
Receptor Agonist ◽  
Ventral Hippocampus ◽  
Agonist Binding

Pre- and postjunctional muscarinic receptor subtypes in the vas deferens of rat

1994 ◽  
Vol 25 (8) ◽  
pp. 1643-1647 ◽  
Author(s):  
H.F. Miranda ◽  
E. Duran ◽  
D. Bustamante ◽  
C. Paeile ◽  
G. Pinardi
Keyword(s):  
Muscarinic Receptor ◽  
Vas Deferens ◽  
Receptor Subtypes ◽  
Muscarinic Receptor Subtypes
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