The effects of cisapride on elemental content in neonatal mouse small intestine in vivo

1996 ◽  
Vol 303 (3) ◽  
pp. 205-208 ◽  
Author(s):  
Anthony J. Spencer ◽  
Michael P. Osborne ◽  
John Stephen
Keyword(s):  
Small Intestine ◽  
Neonatal Mouse ◽  
Elemental Content ◽  
Mouse Small Intestine

scholarly journals In Vivo Imaging of Enteric Neurogenesis in the Deep Tissue of Mouse Small Intestine

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e54814 ◽  
Author(s):  
Kei Goto ◽  
Go Kato ◽  
Isao Kawahara ◽  
Yi Luo ◽  
Koji Obata ◽  
...  
Keyword(s):  
Small Intestine ◽  
In Vivo Imaging ◽  
Deep Tissue ◽  
Mouse Small Intestine

scholarly journals Gata4 Is Essential for the Maintenance of Jejunal-Ileal Identities in the Adult Mouse Small Intestine

2006 ◽  
Vol 26 (23) ◽  
pp. 9060-9070 ◽  
Author(s):  
Tjalling Bosse ◽  
Christina M. Piaseckyj ◽  
Ellen Burghard ◽  
John J. Fialkovich ◽  
Satish Rajagopal ◽  
...  
Keyword(s):  
Small Intestine ◽  
Cell Fate ◽  
Adult Mouse ◽  
Specific Model ◽  
Gene Encoding ◽  
Fatty Acid Binding ◽  
Adult Mice ◽  
Mouse Small Intestine ◽  
Old Mice

ABSTRACTGata4, a member of the zinc finger family of GATA transcription factors, is highly expressed in duodenum and jejunum but is nearly undetectable in distal ileum of adult mice. We show here that the caudal reduction of Gata4 is conserved in humans. To test the hypothesis that the regional expression of Gata4 is critical for the maintenance of jejunal-ileal homeostasis in the adult small intestine in vivo, we established an inducible, intestine-specific model that results in the synthesis of a transcriptionally inactiveGata4mutant. Synthesis of mutant Gata4 in jejuna of 6- to 8-week-old mice resulted in an attenuation of absorptive enterocyte genes normally expressed in jejunum but not in ileum, including those for the anticipated targets liver fatty acid binding protein (Fabp1) and lactase-phlorizin hydrolase (LPH), and a surprising induction of genes normally silent in jejunum but highly expressed in ileum, specifically those involved in bile acid transport. Inactivation ofGata4resulted in an increase in the goblet cell population and a redistribution of the enteroendocrine subpopulations, all toward an ileal phenotype. The gene encoding Math1, a known activator of the secretory cell fate, was induced ∼75% (P< 0.05). Gata4 is thus an important positional signal required for the maintenance of jejunal-ileal identities in the adult mouse small intestine.

Contribution of Radixin to P-Glycoprotein Expression and Transport Activity in Mouse Small Intestine In Vivo

2013 ◽  
Vol 102 (8) ◽  
pp. 2875-2881 ◽  
Author(s):  
Kentaro Yano ◽  
Takumi Tomono ◽  
Riyo Sakai ◽  
Takashi Kano ◽  
Kaori Morimoto ◽  
...  
Keyword(s):  
Small Intestine ◽  
Transport Activity ◽  
P Glycoprotein ◽  
Mouse Small Intestine

scholarly journals Fibroblast growth factor 10 alters the balance between goblet and Paneth cells in the adult mouse small intestine

2015 ◽  
Vol 308 (8) ◽  
pp. G678-G690 ◽  
Author(s):  
Denise Al Alam ◽  
Soula Danopoulos ◽  
Kathy Schall ◽  
Frederic G. Sala ◽  
Dana Almohazey ◽  
...  
Keyword(s):  
Small Intestine ◽  
Cell Differentiation ◽  
Epithelial Cell ◽  
Paneth Cells ◽  
Intestinal Epithelial ◽  
Mouse Small Intestine ◽  
Fibroblast Growth Factor 10 ◽  
Goblet Cell Differentiation

Intestinal epithelial cell renewal relies on the right balance of epithelial cell migration, proliferation, differentiation, and apoptosis. Intestinal epithelial cells consist of absorptive and secretory lineage. The latter is comprised of goblet, Paneth, and enteroendocrine cells. Fibroblast growth factor 10 (FGF10) plays a central role in epithelial cell proliferation, survival, and differentiation in several organs. The expression pattern of FGF10 and its receptors in both human and mouse intestine and their role in small intestine have yet to be investigated. First, we analyzed the expression of FGF10, FGFR1, and FGFR2, in the human ileum and throughout the adult mouse small intestine. We found that FGF10, FGFR1b, and FGFR2b are expressed in the human ileum as well as in the mouse small intestine. We then used transgenic mouse models to overexpress Fgf10 and a soluble form of Fgfr2b, to study the impact of gain or loss of Fgf signaling in the adult small intestine. We demonstrated that overexpression of Fgf10 in vivo and in vitro induces goblet cell differentiation while decreasing Paneth cells. Moreover, FGF10 decreases stem cell markers such as Lgr5, Lrig1, Hopx, Ascl2, and Sox9. FGF10 inhibited Hes1 expression in vitro, suggesting that FGF10 induces goblet cell differentiation likely through the inhibition of Notch signaling. Interestingly, Fgf10 overexpression for 3 days in vivo and in vitro increased the number of Mmp7/Muc2 double-positive cells, suggesting that goblet cells replace Paneth cells. Further studies are needed to determine the mechanism by which Fgf10 alters cell differentiation in the small intestine.

scholarly journals Real‐time in vivo imaging of ischemia‐reperfusion damage in mouse small intestine

2007 ◽  
Vol 21 (6) ◽  
Author(s):  
Yanfang Guan ◽  
Roger T. Worrell ◽  
Timothy A. Pritts ◽  
Marshall H. Montrose
Keyword(s):  
Small Intestine ◽  
Real Time ◽  
In Vivo Imaging ◽  
Ischemia Reperfusion ◽  
Reperfusion Damage ◽  
Mouse Small Intestine

Preparation of cultured astrocytes for x-ray microanalysis

1989 ◽  
Vol 47 ◽  
pp. 988-989
Author(s):  
N.K.R. Smith ◽  
K.E. Hunter ◽  
P. Mobley ◽  
L.P. Felpel
Keyword(s):  
Cultured Cells ◽  
Elemental Content ◽  
Elemental Distribution ◽  
Sprague Dawley ◽  
X Ray ◽  
Cultured Astrocytes ◽  
Freeze Dried ◽  
Ultimate Objective

Electron probe energy dispersive x-ray microanalysis (XRMA) offers a powerful tool for the determination of intracellular elemental content of biological tissue. However, preparation of the tissue specimen , particularly excitable central nervous system (CNS) tissue , for XRMA is rather difficult, as dissection of a sample from the intact organism frequently results in artefacts in elemental distribution. To circumvent the problems inherent in the in vivo preparation, we turned to an in vitro preparation of astrocytes grown in tissue culture. However, preparations of in vitro samples offer a new and unique set of problems. Generally, cultured cells, growing in monolayer, must be harvested by either mechanical or enzymatic procedures, resulting in variable degrees of damage to the cells and compromised intracel1ular elemental distribution. The ultimate objective is to process and analyze unperturbed cells. With the objective of sparing others from some of the same efforts, we are reporting the considerable difficulties we have encountered in attempting to prepare astrocytes for XRMA.Tissue cultures of astrocytes from newborn C57 mice or Sprague Dawley rats were prepared and cultured by standard techniques, usually in T25 flasks, except as noted differently on Cytodex beads or on gelatin. After different preparative procedures, all samples were frozen on brass pins in liquid propane, stored in liquid nitrogen, cryosectioned (0.1 μm), freeze dried, and microanalyzed as previously reported.

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