Satellite cells of growing turkeys: Influence of donor age and sex on proliferation and differentiation in vitro

1990 ◽  
Vol 189 (1) ◽  
pp. 81-86 ◽  
Author(s):  
Matthew E. Doumit ◽  
Douglas C. McFarland ◽  
Richard D. Minshall
Keyword(s):  
Satellite Cells ◽  
Donor Age ◽  
Proliferation And Differentiation ◽  
Age And Sex

scholarly journals miR-194-5p negatively regulates the proliferation and differentiation of rabbit skeletal muscle satellite cells

2020 ◽  
Author(s):  
Yu Shi ◽  
Xudong Mao ◽  
Mingcheng Cai ◽  
Shenqiang Hu ◽  
Xiulan Lai ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cells ◽  
Mammalian Species ◽  
Muscle Growth ◽  
Luciferase Reporter ◽  
Stem Cell Population ◽  
Muscle Satellite Cells ◽  
Skeletal Muscle Satellite Cells ◽  
Proliferation And Differentiation

Abstract Skeletal muscle satellite cells (SMSCs), also known as a multipotential stem cell population, play a crucial role during muscle growth and regeneration. In recent years, numerous miRNAs have been associated with the proliferation and differentiation of SMSCs in a number of mammalian species; however, the regulatory mechanisms of miR-194-5p in rabbit SMSCs still remain scarce. In this study, miR-194-5p was first observed to be highly expressed in the rabbit leg muscle. Furthermore, both the mimics and inhibitor of miR-194-5p were used to explore its role in the proliferation and differentiation of rabbit SMSCs cultured in vitro. Results from both EdU and CCK8 assays showed that miR-194-5p inhibited the proliferation of SMSCs. Meanwhile, Mef2c was identified as a target gene of miR-194-5p based on the dual-luciferase reporter assay results. In addition, upregulation of miR-194-5p decreased the expression levels of Mef2c and MyoG during rabbit SMSCs differentiation on Days 3 and 7 of in vitro culture. Taken together, these data demonstrated that miR-194-5p negatively regulates the proliferation and differentiation of rabbit SMSCs by targeting Mef2c.

In vitro characterization of proliferation and differentiation of trout satellite cells

2010 ◽  
Vol 342 (3) ◽  
pp. 471-477 ◽  
Author(s):  
Jean Charles Gabillard ◽  
Nathalie Sabin ◽  
Gilles Paboeuf
Keyword(s):  
Satellite Cells ◽  
In Vitro Characterization ◽  
Proliferation And Differentiation

Using image-based flow cytometry to monitor satellite cells proliferation and differentiation in vitro

Methods ◽  
2017 ◽  
Vol 112 ◽  
pp. 175-181 ◽  
Author(s):  
Hui-Ying Luk ◽  
Brian K. McFarlin ◽  
Jakob L. Vingren
Keyword(s):  
Flow Cytometry ◽  
Satellite Cells ◽  
Proliferation And Differentiation

scholarly journals KDM4A regulates myogenesis by demethylating H3K9me3 of myogenic regulatory factors

2021 ◽  
Vol 12 (6) ◽  
Author(s):  
Qi Zhu ◽  
Feng Liang ◽  
Shufang Cai ◽  
Xiaorong Luo ◽  
Tianqi Duo ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Proliferation ◽  
Satellite Cells ◽  
Muscle Development ◽  
Kinase Inhibitor ◽  
Myogenic Differentiation ◽  
Myoblast Differentiation ◽  
Proliferation And Differentiation ◽  
H3k9me3 Level

AbstractHistone lysine demethylase 4A (KDM4A) plays a crucial role in regulating cell proliferation, cell differentiation, development and tumorigenesis. However, little is known about the function of KDM4A in muscle development and regeneration. Here, we found that the conditional ablation of KDM4A in skeletal muscle caused impairment of embryonic and postnatal muscle formation. The loss of KDM4A in satellite cells led to defective muscle regeneration and blocked the proliferation and differentiation of satellite cells. Myogenic differentiation and myotube formation in KDM4A-deficient myoblasts were inhibited. Chromatin immunoprecipitation assay revealed that KDM4A promoted myogenesis by removing the histone methylation mark H3K9me3 at MyoD, MyoG and Myf5 locus. Furthermore, inactivation of KDM4A in myoblasts suppressed myoblast differentiation and accelerated H3K9me3 level. Knockdown of KDM4A in vitro reduced myoblast proliferation through enhancing the expression of the cyclin-dependent kinase inhibitor P21 and decreasing the expression of cell cycle regulator Cyclin D1. Together, our findings identify KDM4A as an important regulator for skeletal muscle development and regeneration, orchestrating myogenic cell proliferation and differentiation.

scholarly journals IGF-1 colocalizes with muscle satellite cells following acute exercise in humans

2014 ◽  
Vol 39 (4) ◽  
pp. 514-518 ◽  
Author(s):  
Amanda Grubb ◽  
Sophie Joanisse ◽  
Daniel R. Moore ◽  
Leeann M. Bellamy ◽  
Cameron J. Mitchell ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Stem Cell ◽  
Growth Factor ◽  
Satellite Cells ◽  
Acute Exercise ◽  
Stem Cell Proliferation ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Exercise In Humans

Insulin-like growth factor-1 (IGF-1) regulates stem cell proliferation and differentiation in vitro. The aim of this study was to quantify the change in satellite cell (SC) specific IGF-1 colocalization following exercise. We observed a significant increase (p < 0.05) in the percentage of SC with IGF-1 colocalization from baseline to 72 h after a bout of resistance exercise. This strongly supports a role for IGF-1 in human SC function following exercise.

In vitro characterization of proliferation and differentiation of pig satellite cells

2012 ◽  
Vol 84 (4) ◽  
pp. 322-329 ◽  
Author(s):  
Marie-Hélène Perruchot ◽  
Patrick Ecolan ◽  
Inge Lise Sorensen ◽  
Niels Oksbjerg ◽  
Louis Lefaucheur
Keyword(s):  
Satellite Cells ◽  
In Vitro Characterization ◽  
Proliferation And Differentiation

scholarly journals Prolonged underfeeding of sheep increases myostatin and myogenic regulatory factor Myf-5 in skeletal muscle while IGF-I and myogenin are repressed

2003 ◽  
Vol 176 (3) ◽  
pp. 425-437 ◽  
Author(s):  
F Jeanplong ◽  
JJ Bass ◽  
HK Smith ◽  
SP Kirk ◽  
R Kambadur ◽  
...  
Keyword(s):  
Satellite Cells ◽  
Muscle Growth ◽  
Cell Activity ◽  
Nuclear Antigen ◽  
Myogenic Regulatory Factor ◽  
Proliferation And Differentiation ◽  
Igf I ◽  
Muscle Necrosis

The IGF axis is nutritionally sensitive in vivo and IGFs stimulate myoblast proliferation and differentiation in vitro, while myostatin inhibits these processes in vitro. We hypothesised that underfeeding would reversibly inhibit the myogenic activity of satellite cells in vivo together with decreased IGF-I and increased myostatin in muscle. Satellite cell activity was measured indirectly from the expression of proliferating cell nuclear antigen (PCNA) and the myogenic regulatory factors (MRFs), MyoD, Myf-5 and myogenin. Young sheep were underfed (30% of maintenance) and some killed after 1, 4, 12, 17, 21 and 22 weeks. Remaining underfed animals were then re-fed a control ration of pellets and killed after 2 days, and 1, 6 and 30 weeks. Expression of PCNA and MRFs decreased during the first week of underfeeding. This coincided with reduced IGF-I and myostatin mRNA, and processed myostatin. Subsequently, Myf-5, MyoD, myostatin mRNA and processed myostatin increased, suggesting that satellite cells may have become progressively quiescent. Long-term underfeeding caused muscle necrosis in some animals and IGF-I and MRF expression was increased in these, indicating the activation of satellite cells for muscle repair. Re-feeding initiated rapid muscle growth and increased expression of PCNA, IGF-I and the MRFs concurrently with decreased myostatin proteins. In conclusion, these data indicate that IGF-I and myostatin may work in a coordinated manner to regulate the proliferation, differentiation and quiescence of satellite cells in vivo.

Isolation and culture of wapiti (Cervus elaphus) satellite cells

2000 ◽  
Vol 80 (2) ◽  
pp. 303-309
Author(s):  
N.M. Burton ◽  
L. Shipley ◽  
K. M. Byrne ◽  
J. L. Vierck ◽  
M. V. Dodson
Keyword(s):  
Cell Viability ◽  
Satellite Cells ◽  
Cervus Elaphus ◽  
Horse Serum ◽  
Media Effect ◽  
In Vitro Proliferation ◽  
Pig Skin ◽  
Defined Media ◽  
Proliferation And Differentiation

Myogenic satellite cells (SC) were isolated from the sternomandibularis muscles of two, 227 kg, male wapiti (Cervus elaphus) and studied in primary cell culture. Wapiti-derived SC were capable of attaching to culture substrata and following the myogenic program of proliferation and differentiation to form multinucleated myotubes. Wapiti SC attached equally well to pig skin gelatin (PSG), fibronectin (FN), Matrigel® and plastic (P > 00.05), but cell viability measured at 120 h varied depending on initial substratum type. Pig skin gelatin (0.02% wt vol−1) was chosen for the majority of subsequent experimentation for cost efficiency. The greatest amount of wapiti SC proliferation was observed in media containing 10% (vol/vol−1) horse serum (HS), 15% HS and 15% fetal bovine serum (FBS) (P > 0.05). Wapiti SC proliferated more when exposed to HS and FBS than to sheep serum (SS) (P < 0.05). No proliferation, differentiation or decrease in cell viability was observed in Dulbecco's Modified Eagle Medium (DMEM) + 1% HS, DMEM + 2% HS, DMEM + 3% HS or DMEM + 4% HS (P > 0.05) after 120 h in vitro. Proliferation of SC was doubled when insulin was added to both 10% HS- and 2% HS-containing media (P < 0.05). Although insulin alone in serum-containing media did not promote fusion of wapiti SC, two defined media (ITT and ITT-CF) that contain insulin did promote fusion of wapiti SC cultures. ITT-CF induced 3% fusion of wapiti SC into myotubes, and ITT induced 1% (P < 0.05). There was also an increase in total cell numbers in SC exposed to ITT-CF in comparison with ITT, ovine defined media (ODM) or ovine defined media-Modified (ODM-Mod))(P < 0.05). Although defined media differed in their ability to induce proliferation or differentiation (P < 0.05), the substrata on which the SC were plated did not influence the defined media effect on SC activity (P > 0.05). Satellite cells exposed to ITT and ITT-CF differed morphologically from SC exposed to ODM and ODM-Mod, which may suggest that formulation differences are influencing wapiti-derived SC proliferation and differentiation. Key words: Wapiti, satellite cells, primary culture, myotubes

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