Schistosoma mansoni: Adult worm chemoattraction with barriers of specific molecular weight exclusions

1983 ◽  
Vol 56 (2) ◽  
pp. 255-258 ◽  
Author(s):  
L.K. Eveland ◽  
B. Fried ◽  
L.M. Cohen
Keyword(s):  
Molecular Weight ◽  
Schistosoma Mansoni ◽  
Adult Worm

scholarly journals Immunoelectrophoretic study on common antigens of São Lourenço da Mata and Belo Horizonte strains of Schistosoma mansoni adult worms and Biomphalaria snails

1992 ◽  
Vol 34 (1) ◽  
pp. 49-54
Author(s):  
José Valfrido de Santana ◽  
Yuzuru Iwanaga ◽  
Adriana Maria da Silva Telles ◽  
Maria Risoleta da Silva ◽  
José Felipe Gonçalves ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Schistosoma Mansoni ◽  
Adult Worm ◽  
Biomphalaria Glabrata ◽  
The Other ◽  
Infection Rates ◽  
Crude Extracts ◽  
The Common ◽  
Belo Horizonte ◽  
Common Antigens

Immunoelectrophoretic studies on common antigens were carried out by using rabbits sera immunized against São Lourenço da Mata and Belo Horizonte strains of Schistosoma mansoni adult worms and antigens of Biomphalaria glabrata pigmented (Jaboatão - PE); B. glabrata albino (Belo Horizonte - MG) and B. straminea (São Lourenço da Mata, PE). Furthermore, the reverse approach was proceeded, namely, sera anti Biomphalaria snails produced in rabbits were tested against both strains of Schistosoma adult worm antigens. The analysis of the common antigens between the SLM strains of S. mansoni adult worm and B. glabrata pigmented showed 8 to 9 precipitin bands, 3 bands with B. glabrata albino and only 1 band with B. straminea crude extracts. On the other hand, the BH strain of S. mansoni adult worm antisera produced 6 to 7 bands with B. glabrata pigmented, 5 bands with B. glabrata albino and 1 band with B. straminea antigenic extract. Biomphalaria snails crude extracts were fractionated by Sephadex G-100 column and three fractions were collected from each snail strain. The fractions were tested with anti SLM and BH strains of S. mansoni adult worm sera by immunoelectrophoresis. The common antigens fractionated from Biomphalaria snails crude extracts and those found for both strains of S. mansoni adult worm mostly existed in the first fraction and they were estimated to have molecular weight over 158,000 daltons. In our laboratory, it was found a relationship between the antigenic similarities and experimental infection rates of S. mansoni towards Biomphalaria snails so that more bands were seen with increasing infection rates of S. mansoni.

scholarly journals Schistosoma mansoni: identification of a 46KDa antigen of the schistosomular surface by monoclonal antibody

1994 ◽  
Vol 36 (3) ◽  
pp. 199-204
Author(s):  
V. P. C. P. Toledo ◽  
C. A. P. Tavares ◽  
S. B. Henriques ◽  
E. Nascimento ◽  
M. Resende
Keyword(s):  
Molecular Weight ◽  
Monoclonal Antibody ◽  
Schistosoma Mansoni ◽  
Adult Worm ◽  
Indirect Immunofluorescence ◽  
Newly Transformed Schistosomula

An IgG2a subclass monoclonal antibody, C6G9, was obtained by immunization of BALB/c mice with Schistosoma mansoni egg antigens. With this monoclonal antibody, it was possible to identify a schistosomular antigen with a molecular weight of 46 kilodaltons (KDa), and its expression being evaluated by means of indirect immunofluorescence. The antigen persisted in the integument of the developing schistosomulum, for at least 96 hours post-transformation. The monoclonal antibody also reacted with the cercaria surface, but not with that of adult worm. The C6G9 was also able to mediate significant levels of cytotoxicity in the presence of complement for newly transformed schistosomula.

Immunoprecipitins in human schistosomiasis detected with adult worm antigens released by 3M KC1

1977 ◽  
Vol 51 (1) ◽  
pp. 71-72 ◽  
Author(s):  
Mauro Scapin ◽  
Miriam Tendler
Keyword(s):  
Schistosoma Mansoni ◽  
Adult Worm ◽  
Calcium Chloride ◽  
Surface Proteins ◽  
Immunochemical Characterization ◽  
Human Schistosomiasis

Several methods are being applied for solubilizing adult Schistosoma mansoni antigens which may allow their immunochemical characterization and purification. Studies on antigens and immunoprecipitins in schistosome infections have been carried out with homogenized extracts of adult worms and/or larval forms of Schistosoma mansoni in saline (Biguet et al., 1962; Kagan and Norman, 1963; Silva and Ferri, 1965), or water (Kent, 1963). Kusel (1972) studied surface proteins in S. mansoni membrane, treating the worms with 0·5% saponin in 3% calcium chloride. Murrel et al. (1974) compared by immunodiffusion a crude culture antigen with extracts obtained by freeze-thawing of adult worms and by treatment in 3M KCl.

scholarly journals In vitro effects of artesunate on the survival of worm pairs and egg production of Schistosoma mansoni

2009 ◽  
Vol 83 (1) ◽  
pp. 7-11 ◽  
Author(s):  
Y. Mitsui ◽  
M. Miura ◽  
Y. Aoki
Keyword(s):  
Schistosoma Mansoni ◽  
Adult Worm ◽  
Survival Time ◽  
Egg Production ◽  
Inhibitory Effect ◽  
Male And Female ◽  
Paired Male ◽  
In Vitro Effects ◽  
Paired Female

AbstractThe effect of artesunate (ART) on the survival time of adult worm pairs of Schistosoma mansoni and on their egg output during in vitro culture was assessed. ART significantly decreased the survival time of both paired male and female worms at concentrations of 5, 10, 20 and 40 mg l− 1 during in vitro cultivation. An inhibitory effect of ART on the daily egg output of paired female worms during in vitro cultivation was also observed.

scholarly journals Preliminary analysis of Sm14 in distinct fractions of Schistosoma mansoni adult worm extract

2001 ◽  
Vol 96 (suppl) ◽  
pp. 79-83 ◽  
Author(s):  
Nilton Thaumaturgo ◽  
Mônica Magno Vilar ◽  
Catia Maria Diogo ◽  
Ricardo Edelenyi ◽  
Miriam Tendler
Keyword(s):  
Schistosoma Mansoni ◽  
Adult Worm ◽  
Preliminary Analysis

Effect of praziquantel and oxamniquine treatment on human isotype responses to Schistosoma mansoni : elevated IgE to adult worm

1997 ◽  
Vol 19 (7) ◽  
pp. 333-335 ◽  
Author(s):  
M. WEBSTER ◽  
P.G. FALLON ◽  
A.J.C. FULFORD ◽  
A.E. BUTTERWORTH ◽  
J.H. OUMA ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Adult Worm

scholarly journals Sm14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm14 protein within the adult worm

2002 ◽  
Vol 35 (3) ◽  
pp. 377-381 ◽  
Author(s):  
C.F.A. Brito ◽  
G.C. Oliveira ◽  
S.C. Oliveira ◽  
M. Street ◽  
S. Riengrojpitak ◽  
...  
Keyword(s):  
Gene Expression ◽  
Life Cycle ◽  
Schistosoma Mansoni ◽  
Adult Worm

scholarly journals Schistosoma mansoni: host cell adhesion to the different stages of the parasite, in vivo

1992 ◽  
Vol 34 (3) ◽  
pp. 205-209 ◽  
Author(s):  
Alan L. Melo ◽  
Leógenes H. Pereira ◽  
Conceição R. S. Machado
Keyword(s):  
Cell Adhesion ◽  
Schistosoma Mansoni ◽  
Adult Worm ◽  
Host Cell ◽  
Peritoneal Cavity ◽  
Adherent Cells ◽  
Laboratory Mice ◽  
Free Swimming ◽  
Daughter Sporocyst

The peritoneal cavity of laboratory mice was used to study the phenomenon of host cell adhesion to different evolutive stages of the Schistosoma mansoni (cercaria, adult worm, developing and mature eggs, miracidium, young and mature daughter sporocysts). Material recovered from the peritoneal cavity 30 and 180 min after the inoculation of each evolutive form was examined with the help of a stereomicroscope. The free swimming larvae (cercaria and miracidium), and the evolutive forms producing such larvae (mature egg and mature daughter sporocyst) elicited the host cell adhesion phenomenon. In all forms but cercariae the adherent cells remained as so till 180 minutes after inoculation

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