scholarly journals 1H NMR spectroscopy reveals that mouse Hsp25 has a flexible C-terminal extension of 18 amino acids

FEBS Letters ◽  
1995 ◽  
Vol 369 (2-3) ◽  
pp. 305-310 ◽  
Author(s):  
John A. Carver ◽  
Gennaro Esposito ◽  
Gabriele Schwedersky ◽  
Matthias Gaestel
Keyword(s):  
Amino Acids ◽  
Nmr Spectroscopy ◽  
1H Nmr ◽  
1H Nmr Spectroscopy

Discrimination of Enantiomers of Amides with Two Stereogenic Centers Enabled by Chiral Bisthiourea Derivatives Using 1H NMR Spectroscopy

2021 ◽  
Author(s):  
Hanchang Zhang ◽  
Hongmei Zhao ◽  
Jie Wen ◽  
Zhanbin Zhang ◽  
Pericles Stavropoulos ◽  
...  
Keyword(s):  
Amino Acids ◽  
Nmr Spectroscopy ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Chiral Recognition ◽  
Stereogenic Centers

Enantiomers of a few new amides containing two stereogenic centers have been derived from D- and L-α-amino acids as guests for chiral recognition by 1H NMR spectroscopy. A variety of...

scholarly journals Aromatic amino acids play a harmonizing role in prostate cancer: A metabolomics-based cross-sectional study

2021 ◽  
pp. 741-750
Author(s):  
Ziba Akbari ◽  
Roghayeh Taghipour Dijojin ◽  
Zahra Zamani ◽  
Reza Haji Hosseini ◽  
Mohammad Arjmand
Keyword(s):  
Prostate Cancer ◽  
Amino Acids ◽  
Nmr Spectroscopy ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Aromatic Amino Acids ◽  
Proton Nuclear Magnetic Resonance ◽  
Least Squares Regression ◽  
Cross Sectional ◽  
Metabolomics Data

Background: Prostate cancer (PCa) is a common health problem worldwide. The rate of this disease is likely to grow by 2021. PCa is a heterogeneous disorder, and various biochemical factors contribute to the development of this disease. The metabolome is the complete set of metabolites in a cell or biological sample and represents the downstream end product of the omics. Hence, to model PCa by computational systems biology, a preliminary metabolomics-based study was used to compare the metabolome profile pattern between healthy and PCa men. Objective: This study was carried out to highlight energy metabolism modification and assist the prognosis and treatment of disease with unique biomarkers. Materials and Methods: In this cross-sectional research, 26 men diagnosed with stage-III PCa and 26 healthy men with normal PSA levels were enrolled. Urine was analyzed with proton nuclear magnetic resonance (1H-NMR) spectroscopy, accompanied by the MetaboAnalyst web-based platform tool for metabolomics data analysis. Partial least squares regression discriminant analysis was applied to clarify the separation between the two groups. Outliers were documented and metabolites determined, followed by identifying biochemical pathways. Results: Our findings reveal that modifications in aromatic amino acid metabolism and some of their metabolites have a high potential for use as urinary PCa biomarkers. Tryptophan metabolism (p < 0.001), tyrosine metabolism (p < 0.001), phenylalanine, tyrosine and tryptophan biosynthesis (p < 0.001), phenylalanine metabolism (p = 0.01), ubiquinone and other terpenoid-quinone biosynthesis (p = 0.19), nitrogen metabolism (p = 0.21), and thiamine metabolism (p = 0.41) with Q2 (0.198) and R2 (0.583) were significantly altered. Conclusion: The discriminated metabolites and their pathways play an essential role in PCa causes and harmony. Key words: Metabolomics, Prostate cancer, Aromatic amino acids, 1H-NMR spectroscopy.

scholarly journals Branched-Chain Amino Acids Can Predict Mortality in ICU Sepsis Patients

Nutrients ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 3106
Author(s):  
Alexander Christian Reisinger ◽  
Florian Posch ◽  
Gerald Hackl ◽  
Gunther Marsche ◽  
Harald Sourij ◽  
...  
Keyword(s):  
Amino Acids ◽  
Septic Shock ◽  
Nmr Spectroscopy ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Outcome Prediction ◽  
Branched Chain Amino Acids ◽  
Proton Nuclear Magnetic Resonance ◽  
Branched Chain ◽  
And Control

Sepsis biomarkers and potential therapeutic targets are urgently needed. With proton nuclear magnetic resonance (1H NMR) spectroscopy, several metabolites can be assessed simultaneously. Fifty-three adult medical ICU sepsis patients and 25 ICU controls without sepsis were prospectively enrolled. 1H NMR differences between groups and associations with 28-day and ICU mortality were investigated. In multivariate metabolomic analyses, we found separate clustering of ICU controls and sepsis patients, as well as septic shock survivors and non-survivors. Lipoproteins were significantly different between sepsis and control patients. Levels of the branched-chain amino acids (BCAA) valine (median 43.3 [29.0–53.7] vs. 64.3 [47.7–72.3] normalized signal intensity units; p = 0.005), leucine (57.0 [38.4–71.0] vs. 73.0 [54.3–86.3]; p = 0.034) and isoleucine (15.2 [10.9–21.6] vs. 17.9 [16.1–24.4]; p = 0.048) were lower in patients with septic shock compared to those without. Similarly, BCAA were lower in ICU non-survivors compared to survivors, and BCAA were good discriminators for ICU and 28-day mortality. In uni- and multivariable logistic regression analyses, higher BCAA levels were associated with decreased ICU- and 28-day mortality. In conclusion, metabolomics using 1H NMR spectroscopy showed encouraging potential for personalized medicine in sepsis. BCAA was significantly lower in sepsis non-survivors and may be used as early biomarkers for outcome prediction.

scholarly journals Chiral discrimination of α-hydroxy acids and N-Ts-α-amino acids induced by tetraaza macrocyclic chiral solvating agents by using 1H NMR spectroscopy

2017 ◽  
Vol 15 (7) ◽  
pp. 1642-1650 ◽  
Author(s):  
Caixia Lv ◽  
Lei Feng ◽  
Hongmei Zhao ◽  
Guo Wang ◽  
Pericles Stavropoulos ◽  
...  
Keyword(s):  
Amino Acids ◽  
Nmr Spectroscopy ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Chiral Discrimination ◽  
Hydroxy Acids ◽  
H Nmr

Several α-hydroxy acids and N-Ts-α-amino acids were effectively discriminated in the presence of tetraaza macrocyclic chiral solvating agents (TAMCSAs) 1a–1d by 1H NMR spectroscopy.

Asymetrical Dimer - allyl-palladiu Complexes II. Data from Infrared Spectra and Chemical Behaviour

2008 ◽  
Vol 59 (7) ◽  
Author(s):  
Maria Maganu ◽  
Filip Chiraleu ◽  
Constantin Draghici ◽  
Gheorghe Mihai
Keyword(s):  
Carbon Monoxide ◽  
Nmr Spectroscopy ◽  
1H Nmr ◽  
Infrared Spectra ◽  
Analytical Methods ◽  
1H Nmr Spectroscopy ◽  
Chemical Behaviour ◽  
The Asymmetry ◽  
Pd Complexes

The previous data obtained by 1H-NMR spectroscopy established the existence of an asymmetry of the bond between Pd and p-allylic groups, even in the p-allyl-Pd complexes dimers which are considered usually symmetric dimers. The asymmetry of the bond depends by the substitutes of the allylic group. Other analytical methods were investigated for additional proof of the obtained results. Thus, this paper discusses how this asymmetry would be reflected in the infrared spectra and in the reaction of the complexes with carbon monoxide.

Diastereoselective Reduction of Selected α-substituted β-keto Esters and the Assignment of the Relative Configuration by 1H-NMR Spectroscopy

2020 ◽  
Vol 07 ◽  
Author(s):  
Christian Trapp ◽  
Corinna Schuster ◽  
Chris Drewniok ◽  
Dieter Greif ◽  
Martin Hofrichter
Keyword(s):  
Nmr Spectroscopy ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Selective Reduction ◽  
Reducing Agents ◽  
Relative Configuration ◽  
Curtius Rearrangement ◽  
Keto Esters ◽  
Hydroxy Esters ◽  
Diastereoselective Reduction

Background:: Chiral β-hydroxy esters and α-substituted β-hydroxy esters represent versatile building blocks for pheromones, β-lactam antibiotics and 1,2- or 1,3-aminoalcohols. Objective:: Synthesis of versatile α-substituted β-keto esters and their diastereoselective reduction to the corresponding syn- or anti-α-substituted β-hydroxy esters. Assignment of the relative configuration by NMR-spectroscopy after a CURTIUS rearrangement of α-substituted β-keto esters to 4-substituted 5-methyloxazolidin-2-ones. Method:: Diastereoselective reduction was achieved by using different LEWIS acids (zinc, titanium and cerium) in combination with complex borohydrides as reducing agents. Assignment of the relative configuration was verified by 1H-NMR spectroscopy after CURTIUS-rearrangement of α-substituted β-hydroxy esters to 4-substituted 5-methyloxazolidin-2-ones. Results:: For the syn-selective reduction, titanium tetrachloride (TiCl4) in combination with a pyridine-borane complex (py BH3) led to diastereoselectivities up to 99% dr. High anti-selective reduction was achieved by using cerium trichloride (CeCl3) and steric hindered reducing agents such as lithium triethylborohydride (LiEt3BH). After CURTIUS-rearrangement of each α-substituted β-hydroxy ester to the corresponding 4-substituted 5-methyloxazolidin-2-one, the relative configuration was confirmed by 1H NMR-spectroscopy. Conclusion:: We have expanded the procedure of LEWIS acid-mediated diastereoselective reduction to bulky α-substituents such as the isopropyl group and the electron withdrawing phenyl ring.

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