Origins and three-dimensional distribution of substance P-containing structures on the rat stomach using whole-mount tissue

1984 ◽  
Vol 86 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Hideo Minagawa ◽  
Sadao Shiosaka ◽  
Hiroyoshi Inoue ◽  
Norio Hayashi ◽  
Akinori Kasahara ◽  
...  
Keyword(s):  
Substance P ◽  
Three Dimensional ◽  
Rat Stomach ◽  
Whole Mount ◽  
Dimensional Distribution

scholarly journals The Distribution of Polycomb-Group Proteins During Cell Division and Development in Drosophila Embryos: Impact on Models for Silencing

1998 ◽  
Vol 141 (2) ◽  
pp. 469-481 ◽  
Author(s):  
Peter Buchenau ◽  
Jacob Hodgson ◽  
Helen Strutt ◽  
Donna J. Arndt-Jovin
Keyword(s):  
Target Genes ◽  
Three Dimensional ◽  
Polycomb Group ◽  
Gene Repression ◽  
Sex Combs ◽  
Whole Mount ◽  
Confocal Fluorescence ◽  
Dimensional Distribution ◽  
High Concentrations ◽  
Drosophila Embryos

The subcellular three-dimensional distribution of three polycomb-group (PcG) proteins—polycomb, polyhomeotic and posterior sex combs—in fixed whole-mount Drosophila embryos was analyzed by multicolor confocal fluorescence microscopy. All three proteins are localized in complex patterns of 100 or more loci throughout most of the interphase nuclear volume. The rather narrow distribution of the protein intensities in the vast majority of loci argues against a PcG-mediated sequestration of repressed target genes by aggregation into subnuclear domains. In contrast to the case for PEV repression (Csink, A.K., and S. Henikoff. 1996. Nature. 381:529–531), there is a lack of correlation between the occurrence of PcG proteins and high concentrations of DNA, demonstrating that the silenced genes are not targeted to heterochromatic regions within the nucleus. There is a clear distinction between sites of transcription in the nucleus and sites of PcG binding, supporting the assumption that most PcG binding loci are sites of repressive complexes. Although the PcG proteins maintain tissue-specific repression for up to 14 cell generations, the proteins studied here visibly dissociate from the chromatin during mitosis, and disperse into the cytoplasm in a differential manner. Quantitation of the fluorescence intensities in the whole mount embryos demonstrate that the dissociated proteins are present in the cytoplasm. We determined that <2% of PH remains attached to late metaphase and anaphase chromosomes. Each of the three proteins that were studied has a different rate and extent of dissociation at prophase and reassociation at telophase. These observations have important implications for models of the mechanism and maintenance of PcG- mediated gene repression.

Three-dimensional distribution of substance P-like immunoreactivity in the urinary bladder of rat

1985 ◽  
Vol 63 (3-4) ◽  
pp. 209-222 ◽  
Author(s):  
K. Yokokawa ◽  
M. Sakanaka ◽  
S. Shiosaka ◽  
M. Tohyama ◽  
Y. Shiotani ◽  
...  
Keyword(s):  
Substance P ◽  
Urinary Bladder ◽  
Three Dimensional ◽  
Dimensional Distribution

Applications of Scanning Microscopy in Biomedical Research

1968 ◽  
Vol 26 ◽  
pp. 354-355
Author(s):  
T. L. Hayes
Keyword(s):  
Information Content ◽  
Biomedical Research ◽  
Biomedical Applications ◽  
Three Dimensional ◽  
Dental Enamel ◽  
Resolving Power ◽  
Whole Mount ◽  
Two Parameters ◽  
Content Information ◽  
Sectioned Tissue

Biomedical applications of the scanning electron microscope (SEM) have increased in number quite rapidly over the last several years. Studies have been made of cells, whole mount tissue, sectioned tissue, particles, human chromosomes, microorganisms, dental enamel and skeletal material. Many of the advantages of using this instrument for such investigations come from its ability to produce images that are high in information content. Information about the chemical make-up of the specimen, its electrical properties and its three dimensional architecture all may be represented in such images. Since the biological system is distinctive in its chemistry and often spatially scaled to the resolving power of the SEM, these images are particularly useful in biomedical research.In any form of microscopy there are two parameters that together determine the usefulness of the image. One parameter is the size of the volume being studied or resolving power of the instrument and the other is the amount of information about this volume that is displayed in the image. Both parameters are important in describing the performance of a microscope. The light microscope image, for example, is rich in information content (chemical, spatial, living specimen, etc.) but is very limited in resolving power.

Three-dimensional distribution of ribulose-1, 5-bisphosphate carboxylase/oxygemase during the early development of proplastids in dark-grown Euglena cells transferred to an inorganic medium

1990 ◽  
Vol 48 (3) ◽  
pp. 906-907
Author(s):  
Tomoko Ehara ◽  
Shuji Sumida ◽  
Tetsuaki Osafune ◽  
Eiji Hase
Keyword(s):  
Cell Suspension ◽  
Euglena Gracilis ◽  
Carbon Materials ◽  
Three Dimensional ◽  
Peripheral Region ◽  
Plastid Development ◽  
Bisphosphate Carboxylase ◽  
Inorganic Medium ◽  
Ribulose 1 ◽  
Dimensional Distribution

As shown previously, Euglena cells grown in Hutner’s medium in the dark without agitation accumulate wax as well as paramylum, and contain proplastids showing no internal structure except for a single prothylakoid existing close to the envelope. When the cells are transferred to an inorganic medium containing ammonium salt and the cell suspension is aerated in the dark, the wax was oxidatively metabolized, providing carbon materials and energy 23 for some dark processes of plastid development. Under these conditions, pyrenoid-like structures (called “pro-pyrenoids”) are formed at the sites adjacent to the prolamel larbodies (PLB) localized in the peripheral region of the proplastid. The single prothylakoid becomes paired with a newly formed prothylakoid, and a part of the paired prothylakoids is extended, with foldings, in to the “propyrenoid”. In this study, we observed a concentration of RuBisCO in the “propyrenoid” of Euglena gracilis strain Z using immunoelectron microscopy.

scholarly journals Dip angles of active faults from the surface to the seismogenic zone inferred from a 2D numerical analysis of visco-elasto-plastic models: a case study for the Osaka Plain

2021 ◽  
Vol 73 (1) ◽  
Author(s):  
Hayami Nishiwaki ◽  
Takamoto Okudaira ◽  
Kazuhiko Ishii ◽  
Muneki Mitamura
Keyword(s):  
Urban Areas ◽  
Active Faults ◽  
Three Dimensional ◽  
Seismogenic Zone ◽  
Earthquake Ground Motion ◽  
Convex Curve ◽  
Seismic Reflection Profile ◽  
Geological Structures ◽  
Dimensional Distribution ◽  
Dip Angle

AbstractThe geometries (i.e., dip angles) of active faults from the surface to the seismogenic zone are the most important factors used to evaluate earthquake ground motion, which is crucial for seismic hazard assessments in urban areas. In Osaka, a metropolitan city in Japan, there are several active faults (e.g., the Uemachi and Ikoma faults), which are inferred from the topography, the attitude of active faults in surface trenches, the seismic reflection profile at shallow depths (less than 2 km), and the three-dimensional distribution of the Quaternary sedimentary layers. The Uemachi and Ikoma faults are N–S-striking fault systems with total lengths of 42 km and 38 km, respectively, with the former being located ~ 12 km west of the latter; however, the geometries of each of the active faults within the seismogenic zone are not clear. In this study, to examine the geometries of the Uemachi and Ikoma faults from the surface to the seismogenic zone, we analyze the development of the geological structures of sedimentary layers based on numerical simulations of a two-dimensional visco-elasto-plastic body under a horizontal compressive stress field, including preexisting high-strained weak zones (i.e., faults) and surface sedimentation processes, and evaluate the relationship between the observed geological structures of the Quaternary sediments (i.e., the Osaka Group) in the Osaka Plain and the model results. As a result, we propose geometries of the Uemachi and Ikoma faults from the surface to the seismogenic zone. When the friction coefficient of the faults is ~ 0.5, the dip angles of the Uemachi and Ikoma faults near the surface are ~ 30°–40° and the Uemachi fault has a downward convex curve at the bottom of the seismogenic zone, but does not converge to the Ikoma fault. Based on the analysis in this study, the dip angle of the Uemachi fault zone is estimated to be approximately 30°–40°, which is lower than that estimated in the previous studies. If the active fault has a low angle, the width of the fault plane is long, and thus the estimated seismic moment will be large.

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