Identification and characterization of Schistosoma mansoni antigens recognized by T and B lymphocytes of humans with early active intestinal and/or urinary schistosomiasis

1995 ◽  
Vol 25 (1) ◽  
pp. 113-121 ◽  
Author(s):  
Maged Al-Sherbiny ◽  
Rashika El Ridi ◽  
Nabil I. Guirguis ◽  
David A. Dean
1989 ◽  
Vol 69 (2) ◽  
pp. 249-262 ◽  
Author(s):  
Shohreh Miller ◽  
David M. Rekosh ◽  
Philip T. LoVerde

1996 ◽  
Vol 76 (1-2) ◽  
pp. 73-82 ◽  
Author(s):  
D. Kiang ◽  
N.E. El Ghazalie ◽  
A.M. Medhat ◽  
M. Abdel-Fattah ◽  
A.M. Karim ◽  
...  

BMC Genomics ◽  
2013 ◽  
Vol 14 (1) ◽  
pp. 804 ◽  
Author(s):  
Haiyan Lei ◽  
Tianwei Li ◽  
Guo-Chiuan Hung ◽  
Bingjie Li ◽  
Shien Tsai ◽  
...  

Parasitology ◽  
1987 ◽  
Vol 94 (2) ◽  
pp. 255-268 ◽  
Author(s):  
D. W. Dunne ◽  
Q. D. Bickle

SUMMARYAntisera were produced by immunizing rabbits with either a trichloroacetic acid-soluble fraction, or a high molecular weight (Mr) fraction ofSchistosoma mansoniSEA (a salinesoluble fraction of homogenized egg). Both of these sera reacted monospecifically in immunoelectrophoresis against unfractionated SEA, recognizing a cathodally migrating antigen. This antigen had been identified previously as being responded to byS. mansoni-infected mouse sera, and has been designated K3(Kappa 3). The rabbit antisera were used to partially characterize antigen K3as havingMrin the range > 750 – 70 K, and being resistant to boiling, resistant to the action of proteases, but sensitive to periodate. It partially binds to Concanavalin A. In addition to SEA, the antigen was present in homogenized cercariae and schistosomula, but not adult worms, and it was also present in detergent extracts of intact cercariae and schistosomula. Using an antibody-dependent cell adherence assay, anti-K3serum was found to react with the surface of live cercariae and with the surface of schistosomula recovered from the skin of mice infected up to 48 h previously. Anti-K3serum also reacted with the surface ofS. bovis, S. haematobiumand to a lesser extentS. japonicumschistosomula.


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