Identification and characterization of a polysaccharide-containing antigen fromSchistosoma mansonieggs which cross-reacts with the surface of schistosomula

Parasitology ◽  
1987 ◽  
Vol 94 (2) ◽  
pp. 255-268 ◽  
Author(s):  
D. W. Dunne ◽  
Q. D. Bickle
Keyword(s):  
Molecular Weight ◽  
Schistosoma Mansoni ◽  
High Molecular Weight ◽  
Concanavalin A ◽  
Trichloroacetic Acid ◽  
Soluble Fraction ◽  
Cell Adherence ◽  
Dependent Cell ◽  
Identification And Characterization

SUMMARYAntisera were produced by immunizing rabbits with either a trichloroacetic acid-soluble fraction, or a high molecular weight (Mr) fraction ofSchistosoma mansoniSEA (a salinesoluble fraction of homogenized egg). Both of these sera reacted monospecifically in immunoelectrophoresis against unfractionated SEA, recognizing a cathodally migrating antigen. This antigen had been identified previously as being responded to byS. mansoni-infected mouse sera, and has been designated K3(Kappa 3). The rabbit antisera were used to partially characterize antigen K3as havingMrin the range > 750 – 70 K, and being resistant to boiling, resistant to the action of proteases, but sensitive to periodate. It partially binds to Concanavalin A. In addition to SEA, the antigen was present in homogenized cercariae and schistosomula, but not adult worms, and it was also present in detergent extracts of intact cercariae and schistosomula. Using an antibody-dependent cell adherence assay, anti-K3serum was found to react with the surface of live cercariae and with the surface of schistosomula recovered from the skin of mice infected up to 48 h previously. Anti-K3serum also reacted with the surface ofS. bovis, S. haematobiumand to a lesser extentS. japonicumschistosomula.

Partial characterization of the carbohydrates of the eggs (oncospheres) of the tapeworm, Hymenolepis diminuta

Parasitology ◽  
1995 ◽  
Vol 110 (2) ◽  
pp. 231-239 ◽  
Author(s):  
P. W. Pappas ◽  
G. M. Durka
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Soluble Fraction ◽  
Hymenolepis Diminuta ◽  
Soluble Carbohydrate ◽  
Total Carbohydrate ◽  
Carbohydrate Analysis ◽  
Egg Shells ◽  
Glucose Analysis

SUMMARYThe carbohydrate constituents of whole eggs and fractions derived from the eggs of Hymenolepis diminuta were partly characterized. Whole eggs contained 9·6 ng of carbohydrate (phenol-sulphuric acid-positive material) per egg, and 3·6 ng of glucose. Analysis of hydrolysed eggs by HPLC demonstrated the presence of glucose, galactose, glycerol, N-acetylgalactosamine, and 2-deoxyribose. Isolated egg shells and the KOH-stable, ethanol-precipitable fraction (putative glycogen) of eggs contained 26 and 76%, respectively, of the total carbohydrate associated with eggs. Glucose and galactose were present in both of these samples, but only glucose was present in the KOH-stable, ethanol-precipitable fraction isolated from chemically deshelled eggs; thus, the source of the galactose in the KOH-stable, ethanol-precipitable fraction isolated from whole eggs was the egg-shells. These data confirm earlier reports that the shells contain carbohydrate; the data demonstrate further that only two monosaccharides (glucose and galactose) are present in the shells, and that they are present as high molecular weight polymers. The ethanol-soluble fraction of eggs contained 2·5% of the total carbohydrate in eggs, and glucose accounted for < 1% of the ethanol-soluble carbohydrate. Analysis of the ethanol-soluble fraction by HPLC demonstrated that the predominant monosaccharides were glycerol and mannose, with smaller quantities of 2-deoxyribose and 2-deoxyglucose. The absence of a ‘free pool’ of glucose in eggs, and the presence of large amounts of glycerol and mannose, suggest that the pathways of intermediary carbohydrate metabolism in eggs might be very different from those in adult tapeworms.

Synthesis and characterization of high molecular weight phthalocyanine-PPV copolymers through post-polymerization functionalization

2011 ◽  
Vol 15 (07n08) ◽  
pp. 659-666 ◽  
Author(s):  
Juan-José Cid ◽  
Jan Duchateau ◽  
Ineke Van Severen ◽  
Carolina R. Ganivet ◽  
Gema de la Torre ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Soluble Fraction ◽  
Synthesis And Characterization ◽  
H Nmr ◽  
Ft Ir ◽  
Copolymer 1 ◽  
High Molecular Weight Poly

High molecular weight poly(p-phenylenenevinylene) PPV copolymers laterally substituted with zinc (II) phthalocyanines ( Zn(II)Pc -PPV 1 and 2) have been synthesized by means of post-polymerization functionalization reactions through DCC-mediated esterifications between hydroxy-phthalocyanines 4 and 6 and carboxy-bearer PPV copolymers, comprising 2-methoxy-5-(3,7-dimethyloctyloxy)-1,4-phenylenevinylene (MDMO-PPV) and 1,4-(2-(5-carboxypentyloxy)-5-methoxyphenylenevinylene (CPM-PPV) units in a 9:1 (3) and 1:1 (5) ratio, respectively. The resulting copolymer 1 contains a 7 mol % of Pc molecules, while copolymer 2, which is isolated as the soluble fraction of the reaction with the starting 1:1 copolymer (namely having around 50% of COOH -containing monomeric units), holds a 9 mol % of zinc(II) phthalocyanines. 1 and 2 were fully characterized by 1H NMR, UV-vis and FT-IR spectroscopies.

scholarly journals Identification and Characterization of High-Molecular-Weight Glutenin Subunits from Agropyron intermedium

PLoS ONE ◽  
2014 ◽  
Vol 9 (2) ◽  
pp. e87477 ◽  
Author(s):  
Shuanghe Cao ◽  
Zhixin Li ◽  
Caiyan Gong ◽  
Hong Xu ◽  
Ran Yang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Glutenin Subunits ◽  
Agropyron Intermedium ◽  
Identification And Characterization
Sign in / Sign up

Export Citation Format

Share Document