Determination of hydroxy acids as their copper(II) complexes by reversed-phase liquid chromatography with UV detection

1992 ◽  
Vol 623 (1) ◽  
pp. 55-62 ◽  
Author(s):  
Da Shi Lu ◽  
Wen Yu Feng ◽  
Da Hui Ling ◽  
Wang Zhog Hua
Keyword(s):  
Liquid Chromatography ◽  
Reversed Phase ◽  
Uv Detection ◽  
Hydroxy Acids ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid

scholarly journals Determination of Sulfonamides in Swine Meat by Immunoaffinity Chromatography

2000 ◽  
Vol 83 (4) ◽  
pp. 830-836 ◽  
Author(s):  
Jun Suo Li ◽  
Xi Wang Li ◽  
Jian Xiang Yuan ◽  
Xin Wang
Keyword(s):  
Liquid Chromatography ◽  
Reversed Phase ◽  
Immunoaffinity Chromatography ◽  
Uv Detection ◽  
Reversed Phase Liquid Chromatography ◽  
Specific Antibodies ◽  
Coefficients Of Variation ◽  
Phase Liquid ◽  
Immunoaffinity Columns

Abstract A procedure was developed for the preparation of anti-sulfonamide (SA) group-specific antibodies and immunosorbents. Sulfonamide haptens and conjugates were synthesized by building spacer arms on an N1 group of 4-aminobenzensulfonamide. The anti-SA group-specific antibodies and immunosorbents were prepared successfully. After extraction with methanol–water (8 + 2), sulfamonomethoxine, sulfadimethoxine, and sulfaquinoxaline were cleaned up on immunoaffinity columns and determined by reversed-phase liquid chromatography with UV detection at 270 nm. The recoveries from fortified swine meat (10–100 μg/kg) ranged from 70.8 to 94.1%, with coefficients of variation of 3.4–12.9%. Limits of detection were 1–2 μg/kg.

Liquid Chromatographic Assay of Dimetridazole, Ipronidazole, and Ronidazole in Feeds and Premixes

1992 ◽  
Vol 75 (4) ◽  
pp. 663-666 ◽  
Author(s):  
B Tollomelli ◽  
R Laffi ◽  
S Rossato ◽  
G Colpo
Keyword(s):  
Liquid Chromatography ◽  
Standard Deviation ◽  
Rapid Method ◽  
Reversed Phase ◽  
Uv Detection ◽  
Reversed Phase Liquid Chromatography ◽  
Alumina Column ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract A rapid method has been developed for the determination of dimetridazole (DMZ), ipronidazole (IPZ), and ronidazole (RNZ) in turkey feeds, swine feeds, and premix. The compounds are extracted from samples with warm methanol, the extract is purified over a short alumina column, and an aliquot of the eluate is analyzed by reversed-phase liquid chromatography and UV detection at 309 nm. Alumina cleanup of premixes is not essential, although the resulting chromatograms are cleaner. Recoveries of DMZ from feed formulations ranged from 97 to 103% at the 10.0,50.0, and 100.0 ppm levels, with a standard deviation (SD) of 0.62-3.2%. Recoveries of IPZ ranged from 94.4 to 101.2% at the 5.0,50.0, and 100.0 ppm levels (SD, 0.42-4.4%). RNZ recoveries ranged from 95 to 100.7% at the 6.0,60.0, and 120.0 ppm levels (SD, 1.2-5.33%).

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