Cellular defense reactions of insect hemocytes in vitro: Phagocytosis in a new suspension culture system

SUMMARYAn automated suspension culture system for the cultivation of Plasmodium falciparum is described which retains a degree of flexibility which is absent in other automated culture apparatuses. Not only does this system of cultivation promote rapid multiplication of asexual parasites but also permits the development and maturation of gametocytes. Using a combination of gelatin flotation and N-acetyl glucosamine treatment synchronous development of gametocytes was achieved. The total time for gametocyte maturation in vitro under the conditions provided was 7 days. Stages II and V required 48 h for development whilst I, III and IV needed 24 h each. Mature microgametocytes were relatively long lived in comparison with macrogametocytes. Electron microscopic study of the synchronized stages confirmed the observations of Sinden (1982) but, in addition, we noted the presence of Garnham bodies, a cytostome in all stages and dense spherules in stages I–III similar to the fenestrated buttons in sporozoites and exoerythrocytic forms. The relationship between the number of osmiophilic bodies in the mature gametocytes and their ability to escape from the red cell is reaffirmed.

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In-vitro development of early preantral ovarian follicles from postpuberal mice in a simplified culture system

Reproductive Immunology and Biology ◽

10.3192/jsirib2003.20.1_1 ◽

2005 ◽

Vol 20 (1) ◽

pp. 1-4

Author(s):

Hidemi Kada

Keyword(s):

Culture System ◽

Ovarian Follicles ◽

In Vitro Development ◽

Vitro Development

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Development and Characterization of Explant Tissue Culture System as an In Vitro Model for Uterine Leiomyoma (Fibroid) To Screen Natural Compounds

Journal of scientific research ◽

10.37398/jsr.2020.640131 ◽

2020 ◽

Vol 64 (01) ◽

pp. 117-121

Author(s):

Vivek Pandey ◽

Anjali Rani ◽

Pawan K. Dubey

Keyword(s):

Tissue Culture ◽

Culture System ◽

Uterine Leiomyoma ◽

In Vitro Model ◽

Natural Compounds ◽

Tissue Culture System ◽

Vitro Model

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Supplementation of c-type natriuretic peptide during in vitro growth period benefits the development of murine preantral follicles

Zygote ◽

10.1017/s096719942000060x ◽

2020 ◽

pp. 1-5

Author(s):

Li Ang ◽

Cao Haixia ◽

Li Hongxia ◽

Li Ruijiao ◽

Guo Xingping ◽

...

Keyword(s):

Natriuretic Peptide ◽

Granulosa Cells ◽

Culture System ◽

Early Stage ◽

Developmental Competence ◽

Control Group ◽

Follicle Development ◽

Control Groups ◽

Preantral Follicles

Summary The present study investigated the effects of c-type natriuretic peptide (CNP) on the development of murine preantral follicles during in vitro growth (IVG). Preantral follicles isolated from ovaries of Kunming mice were cultured in vitro. In the culture system, CNP was supplemented in the experimental groups and omitted in the control groups. In Experiment 1, CNP was only supplemented at the early stage and follicle development was evaluated. In Experiments 2 and 3, CNP was supplemented during the whole period of in vitro culture. In Experiment 2, follicle development and oocyte maturity were evaluated. In Experiment 3, follicle development and embryo cleavage after in vitro fertilization (IVF) were assessed. The results showed that in the control groups in all three experiments, granulosa cells migrated from within the follicle and the follicles could not reach the antral stage. In the experimental groups in all three experiments, no migration of granulosa cells was observed and follicle development was assessed as attaining the antral stage, which was significantly superior to that of the control group (P < 0.0001). Oocyte meiotic arrest was effectively maintained, hence giving good developmental competence. In conclusion, CNP supplementation in the culture system during IVG benefited the development of murine preantral follicles.

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Morphometric Registration of Cytotoxic Changes of Epithelial Cells In Vitro: A Methodological Study

Alternatives to Laboratory Animals ◽

10.1177/026119299001700307 ◽

1990 ◽

Vol 17 (3) ◽

pp. 174-176

Author(s):

Lis Andersen ◽

Dorthe Arenholt-Bindslev

Keyword(s):

Culture System ◽

Optimal Allocation ◽

Volume Density ◽

Cell Culture System ◽

Allocation Of Resources ◽

Methodological Study ◽

Epithelial Cell Culture ◽

Coefficients Of Variation ◽

And Control

Quantification of toxicity-induced cytomorphological effects in an epithelial cell culture system is described. Estimates of volume density and star volume of mitochondria and lysosomes are given. Mean volumes (n = 5) and coefficients of variation of these parameters were equal in experimental (TPA-treatment) and control cultures. An optimal allocation of resources for estimating cytomorphometric parameters would be to increase the number of culture flasks.

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A Phytoalexin and Aflatoxin Producing Peanut Seed Culture System

Peanut Science ◽

10.3146/i0095-3679-21-2-13 ◽

1994 ◽

Vol 21 (2) ◽

pp. 130-134 ◽

Cited By ~ 6

Author(s):

S. M. Basha ◽

R. J. Cole ◽

S. K. Pancholy

Keyword(s):

Water Stress ◽

Culture System ◽

Fungal Growth ◽

Aflatoxin Contamination ◽

Peanut Seed ◽

Seed Culture ◽

Murashige And Skoog Medium ◽

Yellow Orange ◽

Increased Susceptibility

Abstract An in vitro seed culture system was established to grow peanut seed of different maturities viz. white, yellow, orange, brown and black, using a modified Murashige and Skoog medium. Under this system peanut seed of yellow, orange, brown and black maturity categories grew to maturity as measured by increase in their size and germinability. In vitro cultured seeds produced significant amounts of phytoalexins and were contaminated with aflatoxins following their inoculation with Aspergillus spp. while the noninoculated sterile controls did not produce any phytoalexins. Exposure of seed cultures to water stress using various concentrations of mannitol (0 to 1 M) and polyethylene glycol 8000 (0-30% w/v) caused a significant decrease in their phytoalexin producing ability, and enhanced fungal growth compared to the nonstressed controls. The seeds that were stressed with mannitol and subsequently inoculated with A. flavus and A. parasiticus showed a significant increase in the aflatoxin contamination of stressed seed compared to the unstressed control. This would indicate that in vitro grown seeds responded to water stress similar to the field grown peanuts by loosing their ability to produce phytoalexins and increased susceptibility to aflatoxin contamination. Hence, this system has a potential application in evaluating peanut genotypes for aflatoxin resistance under water stress.

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