Properties of ribosomal binding sites for phenylalanyl-transfer ribonucleic acid

1967 ◽  
Vol 27 (3) ◽  
pp. 421-430 ◽  
Author(s):  
Nicholas W. Seeds ◽  
James A. Retsema ◽  
Thomas W. Conway
Keyword(s):  
Ribonucleic Acid ◽  
Binding Sites

scholarly journals The binding of spermine to the ribosomes and ribosomal ribonucleic acid from Bacillus stearothermophilus

1969 ◽  
Vol 113 (1) ◽  
pp. 117-121 ◽  
Author(s):  
L. Stevens
Keyword(s):  
Ionic Strength ◽  
Ribosomal Rna ◽  
Ribonucleic Acid ◽  
Binding Sites ◽  
Bacillus Stearothermophilus ◽  
Gel Filtration ◽  
Ribosomal Ribonucleic Acid ◽  
Number Of Binding Sites ◽  
Filtration Technique

1. The total intracellular concentrations of Na+, K+, Mg2+, spermine, spermidine and RNA were measured in Bacillus stearothermophilus. 2. The binding of spermine to ribosomes and to ribosomal RNA from B. stearothermophilus was studied under various conditions by using a gel-filtration technique. 3. The affinity of spermine for ribosomes and for ribosomal RNA decreased with increasing ionic strength of the medium in which they were suspended. 4. The extent of spermine binding did not change appreciably in the temperature range 4–60°. 5. Optimum binding occurred at about pH7·0. 6. The number of binding sites for spermine on either ribosomes or ribosomal RNA was 0·10–0·13/RNA phosphate group. 7. A high proportion of the intracellular spermine is likely to be bound to the ribosomes in vivo; spermine competes with Mg2+ on equal terms for sites on the ribosomes.

Binding Sites on Escherichia coli Ribonucleic Acid Polymerase

1976 ◽  
Vol 4 (4) ◽  
pp. 633-634 ◽  
Author(s):  
A. GRAHAM ◽  
J. GREENE ◽  
P. A. LOWE ◽  
A. D. B. MALCOLM
Keyword(s):  
Escherichia Coli ◽  
Ribonucleic Acid ◽  
Binding Sites
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