Patterns of estrogen and progesterone receptors in rhesus monkey endometrium during secretory phase of normal menstrual cycle and preimplantation stages of gestation

1988 ◽  
Vol 31 (2) ◽  
pp. 223-229 ◽  
Author(s):  
D. Ghosh ◽  
Jayasree Sengupta
Keyword(s):  
Menstrual Cycle ◽  
Rhesus Monkey ◽  
Progesterone Receptors ◽  
Estrogen And Progesterone Receptors ◽  
Secretory Phase ◽  
Normal Menstrual Cycle

Estradiol and progesterone levels in the endometrium and the reproductive tract luminal washing during the pre-implantation stages of gestation in the rhesus monkey

1989 ◽  
Vol 120 (5) ◽  
pp. 649-654 ◽  
Author(s):  
D. Ghosh ◽  
Jayasree Sengupta
Keyword(s):  
Menstrual Cycle ◽  
Rhesus Monkey ◽  
Reproductive Tract ◽  
Sex Steroid ◽  
Menstrual Cycles ◽  
Normal Menstrual Cycle ◽  
Ovulatory Period ◽  
Hormonal Milieu ◽  
Estradiol 17Β ◽  
Estradiol Concentration

Abstract. To obtain an understanding of the sex steroid hormonal milieu during early gestation, the concentrations of estradiol-17β and progesterone were measured in endometria and reproductive tract luminal fluids collected during the post-ovulatory period (days 2 to 6) of conception, and of non-mated menstrual cycles of the rhesus monkey (Macaca mulatta). Estradiol content was found to be higher (P< 0.05) in endometria obtained from conception cycles; day-wise analysis revealed higher (P< 0.05) level of tissue estradiol concentration on day 6 of post-ovulation as compared with the normal menstrual cycle. Endometrial progesterone content remained, however, unaltered in both groups of monkeys and thus relatively lower (P< 0.05) endometrial progesterone to estradiol ratios were seen on days 4, 5 and 6 of gestation compared with normal menstrual cycle. There were no significant changes in the profiles and concentrations of estradiol and progesterone in luminal washings. The typical sex steroid hormonal milieu observed in the endometrium during the peri-implantation stage of gestation in conception cycle may be related to the endometrial differentiation towards implantation in the rhesus monkey.

scholarly journals Endometrial immunocompetent cells in proliferative and secretory phase of normal menstrual cycle

2020 ◽  
Vol 79 (2) ◽  
pp. 296-302
Author(s):  
D. Radović Janošević ◽  
M. Trandafilović ◽  
D. Krtinić ◽  
H. Čolović ◽  
J. Milošević Stevanović ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Immunocompetent Cells ◽  
Secretory Phase ◽  
Normal Menstrual Cycle

scholarly journals Overexpression of estrogen and progesterone receptors as indicator of endometrial receptivity disorder in women with early miscarriage

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Sergey S. Aganezov ◽  
Vera N. Ellinidi ◽  
Kristina Yu. Ponomarenko ◽  
Аnastasiya V. Morotskaya ◽  
Natalia V. Aganezova
Keyword(s):  
Progesterone Receptors ◽  
Main Group ◽  
Control Group ◽  
Morphological Investigation ◽  
Estrogen And Progesterone Receptors ◽  
Secretory Phase ◽  
Normal Expression ◽  
Early Miscarriage ◽  
Focal Fibrosis ◽  
Fertile Women

Background ― The endometrial factor is important in miscarriage (MC) pathogenesis. Objective. To perform a morphofunctional evaluation of endometrium in the patients with uncertain MC cause in anamnesis. Material and methods ― We examined 48 women 23-40 yo [30 (27, 35)]: the main group consisted of 33 patients with early MC in their medical history, while the control group included 15 healthy fertile women. All women had an ovulatory menstrual cycle, normal levels of gonadotropic and thyroid hormones, prolactin, and androgens. Ultrasound and hormonal examinations, along with morphological investigation of endometrial biopsies were performed. Data presented as median with lower and upper quartiles – Me (LQ, UQ). Results ― In 64% (n=21) of women in the main group, we detected inferior secretory phase of their endometrial cycle, focal fibrosis of endometrial stroma; synchronous overexpression of estrogen (ER) and progesterone (PR) receptors in endometrial glands and stroma. ER in the glands was 240 (160, 280) vs. 130 (80, 210) in the stroma, while PR values were 270 (210, 290) in the glands vs. 270 (240, 280) in the stroma: differences from the control group were significant (p<0.01). Remaining women in the main group (36%, n=12) and all women in the control group had full secretory transformation of the endometrium and normal expression of ER and PR. The levels of estradiol and PR in the blood of women with early MC in anamnesis, with different variants of ER and PR expression in the endometrium, did not differ significantly from the control group and corresponded to the reference values. Conclusion ― Nearly two-thirds of women with early miscarriage in anamnesis exhibited overexpression of ER and PR in the endometrium, which may be one of the indicators of its decreased receptivity status.

OESTROGEN AND PROGESTERONE RECEPTOR CONCENTRATIONS IN HUMAN ENDOMETRIUM DURING GESTATION

1979 ◽  
Vol 92 (3) ◽  
pp. 547-552 ◽  
Author(s):  
B. Kreitmann ◽  
F. Bayard
Keyword(s):  
Menstrual Cycle ◽  
Progesterone Receptor ◽  
Nuclear Receptor ◽  
Receptor Binding ◽  
Binding Sites ◽  
Progesterone Receptors ◽  
Oestrogen Receptors ◽  
Secretory Phase ◽  
Ovulatory Period ◽  
Late Secretory Phase

ABSTRACT The concentrations of endometrial oestrogen and progesterone receptors, both in cytosol and in nuclei, have been studied at 8–10 weeks and at 38–40 weeks of gestation. At these two periods the concentration of oestrogen receptors is comparable with the concentration observed during the late secretory phase of the menstrual cycle. At 8–10 weeks of gestation, concentration of progesterone receptors is also comparable with the concentration observed during the secretory phase of the menstrual cycle, but at term there is a significant increase (P < 0.05) and the concentration is then comparable with the concentration observed in the pre-ovulatory period of the menstrual cycle. The receptor binding sites are always predominantly found in nuclei and the increase in progesterone nuclear receptor at term suggests that in man the progesterone withdrawal is not a necessary step in the mechanism of uterine activation during parturition as it is in other species.

ALTERATIONS OF PROTEIN, NUCLEIC ACIDS AND ENZYME ACTIVITIES IN THE HUMAN ENDOMETRIUM DURING THE NORMAL MENSTRUAL CYCLE

1969 ◽  
Vol 61 (4) ◽  
pp. 729-736 ◽  
Author(s):  
H. Schmidt ◽  
P. Berle ◽  
K. D. Voigt
Keyword(s):  
Menstrual Cycle ◽  
Nucleic Acids ◽  
Enzyme Activities ◽  
Biochemical Parameter ◽  
Cell Number ◽  
Secretory Phase ◽  
Endometrial Cells ◽  
Normal Menstrual Cycle ◽  
Cyclic Variations ◽  
Glucose 6 Phosphate Dehydrogenase

ABSTRACT In twenty-one women with a normal menstrual cycle determinations of protein, of nucleic acids and of some enzymes of energy metabolism have been performed on endometrial tissue. The calculation of enzyme activities is based on the DNA content which represents a biochemical parameter of the cell number. The glucose-6-phosphate dehydrogenase and the fructose-1,6-diphosphate aldolase show markedly increased activities during the secretory phase of the cycle. This suggests that the ovarian hormones induce a considerable stimulation of carbohydrate metabolism of the endometrial cells. Isocitrate dehydrogenase activity shows a transient but marked increase immediately after ovulation. There is also activation of an amino peptidase during the secretory phase of the cycle. The data presented are compatible with previous observations on the cyclic variations of these parameters in the rat uterus.

CONCENTRATIONS OF PROSTAGLANDINS F2α AND E2 IN THE ENDOMETRIUM THROUGHOUT THE HUMAN MENSTRUAL CYCLE, AFTER THE ADMINISTRATION OF CLOMIPHENE OR AN OESTROGEN–PROGESTOGEN PILL AND IN EARLY PREGNANCY

1978 ◽  
Vol 77 (3) ◽  
pp. 361-371 ◽  
Author(s):  
J. B. MAATHUIS ◽  
R. W. KELLY
Keyword(s):  
Menstrual Cycle ◽  
Endometrial Tissue ◽  
Gas Chromatography Mass Spectrometry ◽  
Secretory Phase ◽  
Decidual Tissue ◽  
Normal Menstrual Cycle ◽  
Menstruating Women ◽  
High Concentrations ◽  
Normal Women ◽  
Human Menstrual Cycle

The concentrations of prostaglandins F2α (PGF) and E2 (PGE) were measured by gas chromatography–mass spectrometry in endometrial tissue obtained from 45 normal women at various stages of the menstrual cycle. During the proliferative stages, the concentration of PGF in the endometrium was correlated with the concentration of oestradiol in the plasma. The concentration of PGF during the mid-secretory stage (mean, 2·047, range, 0·549–4·344 μg/g fresh endometrial tissue) was significantly higher than the concentrations during the late proliferative and late secretory stages. The endometrial concentration of PGE did not show a cyclic variation. The concentrations of PGF and PGE in samples of endometrium collected after the administration of clomiphene were significantly lower than the concentrations observed in endometrial tissue obtained from normally menstruating women in the mid-proliferative period. The administration of an oestrogen–progestogen pill resulted in higher endometrial concentrations of PGE than were measured in the mid-secretory phase. The concentrations of PGF and PGE in decidual tissue (conceptual age 3–10 weeks) were lower than those measured at any stage of the normal menstrual cycle. During the human menstrual cycle, high levels of oestradiol and progesterone are related to high endometrial levels of PGF but not PGE. The presence of a conceptus apparently blocks the effect of high concentrations of ovarian steroids on the synthesis or catabolism of prostaglandins.

scholarly journals poFUT1 promotes uterine angiogenesis and vascular remodeling via enhancing the O-fucosylation on uPA

2019 ◽  
Vol 10 (10) ◽  
Author(s):  
Dandan Zhang ◽  
Yu Yang ◽  
Caixia Liang ◽  
Jianwei Liu ◽  
Hao Wang ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Vascular Remodeling ◽  
Secretory Phase ◽  
Uterine Endometrium ◽  
Healthy Pregnancy ◽  
Dynamic Expression ◽  
Normal Menstrual Cycle ◽  
Key Enzyme ◽  
Rhoa Signaling ◽  
Insight Into

Abstract Uterine angiogenesis and vascular remodeling play critical roles in determing the normal menstrual cycle and successful pregnancy. Poor uterine angiogenesis usually results in pregnancy failure. Protein O-fucosyltransferase 1 (poFUT1) is the key enzyme responsible for O-fucosylated glycan biosynthesis on glycoproteins. However, the dynamic expression and regulation of poFUT1 on the uterine angiogenesis and vascular remodeling remain unknown. Here, we showed that the enlargement of the vascular lumen in the secretory phase was greater than that in the proliferative phase of the uterine endometrium during menstrual cycle; whereas there was a narrower vessel lumen and fewer blood vessels in the decidua from miscarriage patients than in that from healthy pregnancy women. Additionally, the expression of poFUT1 was increased in the uterine endometrium during the secretory phase compared with that in the proliferation phase, and its expression was decreased in the uterus of miscarriage patients compared with that of the healthy pregnancy women. Using hESCs and a mouse model, we demonstrated that poFUT1 increased the O-fucosylation on uPA, and activated of the RhoA signaling pathway, thus facilitating uterine angiogenesis and vascular remodeling. We also provide evidence that poFUT1 promotes hESCs angiogenesis by the decreased stemness of hESCs. These findings reveal a new insight into the uterine angiogenesis and vascular remodeling. The study suggests that poFUT1 could be seen as a novel potential diagnostic and therapeutic target for miscarriage.

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