The kinetics of X-ray-induced somatic crossing-over in Drosophila melanogaster and the effects of dose fractionation

Author(s):  
J.R. Merriam ◽  
W.E. Fyffe
Genetics ◽  
1974 ◽  
Vol 76 (4) ◽  
pp. 767-774
Author(s):  
J H Postlethwait ◽  
J R Girton

ABSTRACT Development of the homoeotic mutation, aristapedia (ss  a), was investigated by means of genetic mosaics. The wild-type alleles of aristapedia and the bristle markers yellow, singed, and forked were removed from cells at different times in development by X-ray induced somatic crossing-over. The phenotype of the resulting clones was examined in order to ascertain whether it was leg or antenna. The y sn f; ssa clones showed a leg phenotype if induced before the mid-third instar, but showed an antennal phenotype if induced after this time. Late non-expression of ss  a may be due either to an influence of surrounding ss  + tissues on the small ss  a clones, or to a persistence of the effect of ss  + for one or two cell generations after it is removed from a cell line.


2019 ◽  
Author(s):  
Hao Wu ◽  
Jeffrey Ting ◽  
Siqi Meng ◽  
Matthew Tirrell

We have directly observed the <i>in situ</i> self-assembly kinetics of polyelectrolyte complex (PEC) micelles by synchrotron time-resolved small-angle X-ray scattering, equipped with a stopped-flow device that provides millisecond temporal resolution. This work has elucidated one general kinetic pathway for the process of PEC micelle formation, which provides useful physical insights for increasing our fundamental understanding of complexation and self-assembly dynamics driven by electrostatic interactions that occur on ultrafast timescales.


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