Synergistic effects of sequential treatment with methyl jasmonate, salicylic acid and yeast extract on benzophenanthridine alkaloid accumulation and protein expression in Eschscholtzia californica suspension cultures

2008 ◽  
Vol 135 (1) ◽  
pp. 117-122 ◽  
Author(s):  
Hwa-Young Cho ◽  
Seok Young Son ◽  
Hong Soon Rhee ◽  
Sung-Yong H. Yoon ◽  
Carolyn W.T. Lee-Parsons ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Protein Expression ◽  
Methyl Jasmonate ◽  
Yeast Extract ◽  
Synergistic Effects ◽  
Suspension Cultures ◽  
Sequential Treatment ◽  
Eschscholtzia Californica ◽  
Benzophenanthridine Alkaloid

Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures

2007 ◽  
Vol 29 (12) ◽  
pp. 2001-2005 ◽  
Author(s):  
Hwa-Young Cho ◽  
Carolyn W. T. Lee-Parsons ◽  
Sung-Yong H. Yoon ◽  
Hong Soon Rhee ◽  
Jong Moon Park
Keyword(s):  
Protein Expression ◽  
Suspension Cultures ◽  
Alkaloid Production ◽  
Eschscholtzia Californica ◽  
Benzophenanthridine Alkaloid

scholarly journals Enhancement of Macarpine Production in Eschscholzia Californica Suspension Cultures under Salicylic Acid Elicitation and Precursor Supplementation

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1261
Author(s):  
Andrea Balažová ◽  
Júlia Urdová ◽  
Vladimír Forman ◽  
Pavel Mučaji
Keyword(s):  
Gene Expression ◽  
Salicylic Acid ◽  
Biological Activities ◽  
Suspension Cultures ◽  
Sequential Treatment ◽  
Alkaloid Production ◽  
Simultaneous Treatment ◽  
Eschscholzia Californica ◽  
A Minor ◽  
Benzophenanthridine Alkaloid

Macarpine is a minor benzophenanthridine alkaloid with interesting biological activities, which is produced in only a few species of the Papaveraceae family, including Eschscholzia californica. Our present study was focused on the enhancement of macarpine production in E. californica suspension cultures using three elicitation models: salicylic acid (SA) (4; 6; 8 mg/L) elicitation, and simultaneous or sequential combinations of SA and L-tyrosine (1 mmol/L). Sanguinarine production was assessed along with macarpine formation in elicited suspension cultures. Alkaloid production was evaluated after 24, 48 and 72 h of elicitation. Among the tested elicitation models, the SA (4 mg/L), supported by L-tyrosine, stimulated sanguinarine and macarpine production the most efficiently. While sequential treatment led to a peak accumulation of sanguinarine at 24 h and macarpine at 48 h, simultaneous treatment resulted in maximum sanguinarine accumulation at 48 h and macarpine at 72 h. The effect of SA elicitation and precursor supplementation was evaluated also based on the gene expression of 4′-OMT, CYP719A2, and CYP719A3. The gene expression of investigated enzymes was increased at all used elicitation models and their changes correlated with sanguinarine but not macarpine accumulation.

scholarly journals A Study On The Effect of Different Elicitors On Capsaicin Accumulation in Cell Suspension Cultures of Capsicum Assamicum (Bhut Jolokia)

2021 ◽  
Author(s):  
Swet Nisha ◽  
Ajitabh Bora ◽  
HK Gogoi ◽  
SK Dwivedi ◽  
PJ Handique
Keyword(s):  
Salicylic Acid ◽  
Suspension Culture ◽  
Methyl Jasmonate ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Sinapic Acid ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Exponential Phase ◽  
Bhut Jolokia

Abstract Elicitation of cell suspension cultures of Capsicum assamicum (Bhut Jolokia) for enhancement of capsaicin content was tried using different elicitors such as cellulase, vanillin, methyl jasmonate, salicylic acid and sinapic acid in different concentrations for 24, 48 and 72 hours. Cell suspension culture was established in B5 media supplemented with 3.5 mM 2,4-D (2,4-diphenoxyacetic acid) and 1.1 mM Kin and elicitors were introduced at the end of exponential phase. All the elicitors, except methyl jasmonate, led to significant increase in production of capsaicin. Sinapic acid, when added in 22 µM concentration and incubated for 24 hours, led to highest capsaicin accumulation of 0.5% (5068 µg/g) which was highest among all the treatments.

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