Determination of thermodynamic data by microcalorimetry: the michaelis constant of glucose oxidase immobilized on various carriers

1985 ◽  
Vol 94 (1) ◽  
pp. 169-177 ◽  
Author(s):  
L. Schachinger ◽  
E. Altmann ◽  
B. Diepold ◽  
H. Klöter
Keyword(s):  
Glucose Oxidase ◽  
Thermodynamic Data ◽  
Michaelis Constant

Preparation of enzyme electrode for D-glucose determination by immobilization of glucose oxidase on collagen membrane

1990 ◽  
Vol 55 (10) ◽  
pp. 2568-2574 ◽  
Author(s):  
Eva Vrbová ◽  
Miroslav Marek
Keyword(s):  
Glucose Oxidase ◽  
Oxygen Sensor ◽  
Immobilized Enzyme ◽  
Sensor Response ◽  
Enzyme Electrode ◽  
Collagen Membrane ◽  
Michaelis Constant ◽  
Glucose Determination ◽  
The Stability

An enzyme electrode for the determination of D-glucose was prepared by immobilization of glucose oxidase (EC 1.1.3.4.) on an activated collagen membrane using glutaraldehyde and the Ugi reaction resp. and by subsequent fixation of the membrane to an oxygen sensor of the Clark type. Two different procedures for the modification of the support, the composition of the reaction mixture and the immobilization time were examined. The electrode prepared was tested as regards the effect of pH and temperature on the magnitude of the response. The range of the linear dependence of the sensor response on substrate concentration (1.7 . 10-5 - 2.0 . 10-3), the apparent Michaelis constant of the immobilized enzyme (KM(app.) = 4.16 . 10-3 mol dm-3) and the stability of the biosensor as function of storage mode and number of assays performed with one electrode were determined. In view of the high stability and linear range of the concentration dependence the enzyme electrode is suitable for the determination of D-glucose in samples analyzed in agricultural and food laboratories.

Determination of the Apparent Michaelis Constant of Glucose Oxidase Immobilized on a Microelectrode with Respect to Oxygen

2010 ◽  
Vol 22 (9) ◽  
pp. 927-930 ◽  
Author(s):  
Tomoyuki Yasukawa ◽  
Keisuke Goto ◽  
Fumio Mizutani
Keyword(s):  
Glucose Oxidase ◽  
Michaelis Constant

scholarly journals Preparation of some immobilized linked enzyme systems and their use in the automated determination of disaccharides

1974 ◽  
Vol 137 (1) ◽  
pp. 25-32 ◽  
Author(s):  
D. J. Inman ◽  
W. E. Hornby
Keyword(s):  
Glucose Oxidase ◽  
Enzyme System ◽  
Inside Surface ◽  
Enzyme Systems ◽  
Nylon Tube ◽  
In Series ◽  
Automated Determination

1. Glucose oxidase (EC 1.1.3.4), amyloglucosidase (EC 3.2.1.3), invertase (EC 3.2.1.26) and β-galactosidase (EC 3.2.1.23) were covalently attached via glutaraldehyde to the inside surface of nylon tube. 2. The linked enzyme system, comprising invertase immobilized within a nylon tube acting in series with glucose oxidase immobilized in a similar way, was used for the automated determination of sucrose. 3. The linked enzyme system, comprising β-galactosidase immobilized within a nylon tube acting in series with glucose oxidase immobilized in a similar way, was used for the automated determination of lactose. 4. The linked enzyme system, comprising amyloglucosidase immobilized within a nylon tube acting in series with glucose oxidase immobilized in a similar way, was used for the automated determination of maltose. 5. Mixtures of glucose oxidase and amyloglucosidase were immobilized within the same piece of nylon tube and used for the automated determination of maltose. 6. Mixtures of glucose oxidase and invertase were immobilized within the same piece of nylon tube and used for the automated determination of sucrose.

Enzyme thermistor determination of glucose in serum using immobilized glucose oxidase

1977 ◽  
Vol 81 (2) ◽  
pp. 163-175 ◽  
Author(s):  
B. Danielsson ◽  
Kerstin Gadd ◽  
B. Mattiasson ◽  
K. Mosbach
Keyword(s):  
Glucose Oxidase ◽  
Immobilized Glucose Oxidase ◽  
Enzyme Thermistor
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