2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced appetite suppression in the sprague-dawley rat is not a direct effect on feed intake regulation in the brain

The effect of hexachlorophene (HCP; 2,2'-methylenebis (3,4,6-trichlorophenol), cuprizone (CPZ; bicyclohexone oxaldihydrazone) and triethyl tin (TET; triethyl tin sulphate) in producing vacuoles in the brain of the Sprague-Dawley rat has been quantified by image analysis of the extent of the spongy change in the white matter. The state of the astrocytes was assessed by immunocytochemical staining for glial fibrillary acidic protein (GFAP). HCP and TET caused a dose-related spongiosis, but cuprizone had no significant effect on the brain. With chronic HCP treatment, the spongiosis was accompanied by astrocyte hypertrophy and proliferation, and the extent of the gliotic reaction was related to the dose of HCP. The results demonstrate that HCP can produce and maintain astrocyte proliferation in the rat brain. Such an agent was required for use in an investigation of a putative tumour promoter in the rat.

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Chemically induced hepatic cytosol from the Sprague—Dawley rat: evidence for specific binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin to components kinetically distinct from the Ah receptor

Toxicology Letters ◽

10.1016/0378-4274(90)90072-t ◽

1990 ◽

Vol 51 (3) ◽

pp. 295-302 ◽

Cited By ~ 3

Author(s):

James P. Landers ◽

Lisa M. Birse ◽

Jamie S. Nakai ◽

Martha J. Winhall ◽

Nigel J. Bunce

Keyword(s):

Specific Binding ◽

Ah Receptor ◽

Sprague Dawley ◽

Sprague Dawley Rat ◽

Chemically Induced ◽

Tetrachlorodibenzo P Dioxin

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Permeability of the microcirculation in area postrema of rat

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100103802 ◽

1988 ◽

Vol 46 ◽

pp. 348-349

Author(s):

Shams M. Ghoneim ◽

Frank M. Faraci ◽

Gary L. Baumbach

Keyword(s):

Brain Stem ◽

Area Postrema ◽

Upper Body ◽

Sprague Dawley ◽

Sodium Cacodylate ◽

Acute Hypertension ◽

Sprague Dawley Rats ◽

Ultrastructural Characteristics ◽

The Brain ◽

Adjacent Brain

The area postrema is a circumventricular organ in the brain stem and is one of the regions in the brain that lacks a fully functional blood-brain barrier. Recently, we found that disruption of the microcirculation during acute hypertension is greater in area postrema than in the adjacent brain stem. In contrast, hyperosmolar disruption of the microcirculation is greater in brain stem. The objective of this study was to compare ultrastructural characteristics of the microcirculation in area postrema and adjacent brain stem.We studied 5 Sprague-Dawley rats. Horseradish peroxidase was injected intravenously and allowed to circulate for 1, 5 or 15 minutes. Following perfusion of the upper body with 2.25% glutaraldehyde in 0.1 M sodium cacodylate, the brain stem was removed, embedded in agar, and chopped into 50-70 μm sections with a TC-Sorvall tissue chopper. Sections of brain stem were incubated for 1 hour in a solution of 3,3' diaminobenzidine tetrahydrochloride (0.05%) in 0.05M Tris buffer with 1% H2O2.

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