A differential extraction method for the isolation of host nucleic acids from TMV-infected tobacco plants

Virology ◽  
1970 ◽  
Vol 42 (3) ◽  
pp. 662-667 ◽  
Author(s):  
G. Bagi ◽  
A. Gulyás ◽  
F. Solymosy
Keyword(s):  
Nucleic Acids ◽  
Extraction Method ◽  
Tobacco Plants ◽  
Differential Extraction ◽  
Infected Tobacco

scholarly journals STUDY OF RESPIRATION IN HEALTHY AND MOSAIC-INFECTED TOBACCO PLANTS

1942 ◽  
Vol 17 (2) ◽  
pp. 267-277 ◽  
Author(s):  
Violette F. C. -Glasstone
Keyword(s):  
Tobacco Plants ◽  
Infected Tobacco

SOME EFFECTS OF FORMALDEHYDE ON POTATO VIRUS Y IN VITRO, AND ABILITY OF APHIDS TO TRANSMIT THE VIRUS WHEN THEIR STYLETS ARE TREATED WITH FORMALDEHYDE

1955 ◽  
Vol 1 (9) ◽  
pp. 783-793 ◽  
Author(s):  
R. H. E. Bradley ◽  
R. Y. Ganong
Keyword(s):  
Potato Virus ◽  
Myzus Persicae ◽  
Potato Virus Y ◽  
Tobacco Plants ◽  
Considerable Distance ◽  
Formaldehyde Treatment ◽  
Aphid Vectors ◽  
Infected Tobacco

Potato virus Y (PVY) was made noninfective by incubation with formaldehyde in vitro. Yet this noninfective virus reacted with PVY antiserum and caused antibodies to be produced in rabbits as readily as infective PVY. A method is described for baring the stylets of living aphids beyond the end of the labium, which normally encloses the stylets. Specimens of Myzus persicae (Sulz.) infective with PVY were made noninfective by treating the stylets for 30 sec. with concentrations of formaldehyde as low as 0.03%; and 0.25% formaldehyde caused the same effect in five seconds. Aphids were also made noninfective when the proboscis with the tip of the stylets bared was treated with formaldehyde, even after the stylets had been inserted a considerable distance into infected tobacco plants. By contrast, aphids usually remained infective when the proboscis with the stylets enclosed in the labium was treated with similar concentrations of formaldehyde. However, formaldehyde treatment of the stylets did not affect the ability of aphids immediately thereafter to acquire and transmit PVY. These results are compatible with the hypothesis that viruses transmitted like PVY are carried by the stylets of their aphid vectors.

scholarly journals Biosynthesis of novel phytochemicals in tobacco plant infected with tobacco mosaic tobamovirus (TMV)

Plant Omics ◽  
2018 ◽  
pp. 128-134
Author(s):  
Reham M. Mostafa ◽  
Heba S. Essawy
Keyword(s):  
Free Radicals ◽  
Diagnostic Tool ◽  
Tobacco Plant ◽  
Control Analysis ◽  
Protein Patterns ◽  
Tobacco Plants ◽  
Datura Metel ◽  
Sds Page ◽  
Local Lesions ◽  
Infected Tobacco

The main purpose of this work was to study the effect of TMV infection on physiology of active gradient photochemicals and protein expression in infected tobacco plants. Impact of Tobacco mosaic tobamovirus (TMV) on active gradient photochemicals quantitative and qualitative was evaluated in Nicotiana tobaccum cv. white burly. First, the TMV samples were isolated from single local lesions of infected leaves of N. glutinosa. Then, the N. tobaccum cv. white burly plants were inoculated with TMV. The infected plants showed severe systemic mosaic symptoms and reduction of leave size. We used Datura metel as a diagnostic tool-plant (indicator) for mosaic virus because of its vast exhibitory ability to show the symptoms incited by viruses. It was confirmed that these symptoms were due to the effect of TMV virus, comparing with Datura plant (as control). Analysis of TMV infected leaves by GC-mass detected biosynthesis of novel photochemicals (2-cyclopenten-1-one, Furfural, Indene, Pyrrole, Benzonitrile, Guaiacol and Oxime, methoxy-phenyl) that could not be detected in healthy plants. Furthermore, a 56.17% decreased in nicotine content was observed in infected plants compared with healthy ones. Also, increase of soluble protein contents was observed in infected leaves in response to TMV infection, compared with healthy ones. Alterations in protein patterns were observed in N. tabaccum leaves in response to TMV infection using SDS PAGE. Several secondary bioactive compounds were also found to hold important functions in infected plants. For example, flavonoids could protect against free radicals generated during photosynthesis. Terpenoids may attract pollinators or seed dispersers, or inhibit competing plants. Alkaloids usually ward-off herbivore animals or insect attacks (phytoalexins).

Changes in key enzymes of viral-RNA biosynthesis in chloroplasts from PVY and TMV infected tobacco plants

2005 ◽  
Vol 49 (3) ◽  
pp. 471-474 ◽  
Author(s):  
M. Sindelarova ◽  
L. Sindelar ◽  
N. Wilhelmova ◽  
D. Prochazkova
Keyword(s):  
Viral Rna ◽  
Tobacco Plants ◽  
Key Enzymes ◽  
Rna Biosynthesis ◽  
Infected Tobacco

scholarly journals Pollen Transmission of Asparagus virus 2 (AV-2) May Facilitate Mixed Infection by Two AV-2 Isolates in Asparagus Plants

2014 ◽  
Vol 104 (9) ◽  
pp. 1001-1006 ◽  
Author(s):  
Ryusuke Kawamura ◽  
Hanako Shimura ◽  
Tomofumi Mochizuki ◽  
Satoshi T. Ohki ◽  
Chikara Masuta
Keyword(s):  
Mixed Infection ◽  
Nicotiana Benthamiana ◽  
Pollen Grains ◽  
Mixed Infections ◽  
Sequence Analyses ◽  
Tobacco Plants ◽  
Pollen Transmission ◽  
Infected Tobacco ◽  
Immunohistochemical Analyses

Asparagus virus 2 (AV-2) is a member of the genus Ilarvirus and thought to induce the asparagus decline syndrome. AV-2 is known to be transmitted by seed, and the possibility of pollen transmission was proposed 25 years ago but not verified. In AV-2 sequence analyses, we have unexpectedly found mixed infection by two distinct AV-2 isolates in two asparagus plants. Because mixed infections by two related viruses are normally prevented by cross protection, we suspected that pollen transmission of AV-2 is involved in mixed infection. Immunohistochemical analyses and in situ hybridization using AV-2-infected tobacco plants revealed that AV-2 was localized in the meristem and associated with pollen grains. To experimentally produce a mixed infection via pollen transmission, two Nicotiana benthamiana plants that were infected with each of two AV-2 isolates were crossed. Derived cleaved-amplified polymorphic sequence analysis identified each AV-2 isolate in the progeny seedlings, suggesting that pollen transmission could indeed result in a mixed infection, at least in N. benthamiana.

scholarly journals CERTAIN ENZYMATIC ACTIVITIES OF NORMAL AND MOSAIC INFECTED TOBACCO PLANTS

1942 ◽  
Vol 25 (4) ◽  
pp. 649-661 ◽  
Author(s):  
F. Lyle Wynd
Keyword(s):  
Normal Tissue ◽  
Virus Protein ◽  
Great Decrease ◽  
Tobacco Plants ◽  
Virus Particles ◽  
Abnormal Metabolism ◽  
Metabolic Activities ◽  
Increased Activity ◽  
Infected Tobacco ◽  
Leaf Juice

The lower leaves of tobacco plants were inoculated with leaf mosaic virus and the activities of oxygenase, peroxidase, catalase, and invertase were followed in leaves of comparable age at intervals of 2 or 3 days over a period of 21 days. The inoculated leaves exhibited a great decrease relative to normal tissue in the activity of oxygenase and peroxidase on the 6th day. Younger leaves showed this minimum at a progressively later date. A great decrease in the activities of these enzymes was attained by the 14th to the 18th day. This maximum was followed by a decrease. Catalase exhibited an increased activity which reached a maximum at about the 8th day. A second maximum was observed on the 16th to the 18th day. Invertase reached a minimum, relative to normal plants, on about the 8th day. A second minimum was approached on the 16th to the 18th day. These data show that profound disturbances in the physiology of infected plants occur many days before the leaf juice attains an infectious concentration of virus. The observed activities could not be due therefore to metabolic activities of the virus particles themselves. Since infectivity is attained only after a period of profound physiological disturbance, it seems possible that the virus protein develops as a product of abnormal metabolism.

scholarly journals Spatial Distribution of Acidic Chitinases and Their Messenger RNAs in Tobacco Plants Infected with Cherry Leaf Roll Virus

1997 ◽  
Vol 10 (6) ◽  
pp. 784-788 ◽  
Author(s):  
J. M. Balsalobre ◽  
P. Más ◽  
M. A. Sánchez-Pina ◽  
V. Pallás
Keyword(s):  
Spatial Distribution ◽  
Leaf Roll ◽  
Positive Association ◽  
Chitinase Gene ◽  
Messenger Rnas ◽  
Tobacco Plants ◽  
Accumulation Pattern ◽  
Tissue Printing ◽  
Cherry Leaf Roll Virus ◽  
Infected Tobacco

Cherry leaf roll virus (CLRV) infection in Nicotiana tabacum cv. Xanthi nc plants induces the expression of, among others, basic and acidic chitinases at 3 d.p.i. (days postinoculation) and 8 d.p.i. in inoculated and uninoculated, systemically infected leaves, respectively. The spatial distribution of the acidictobacco chitinases (PR-P and PR-Q) mRNAs in CLRV-infected tobacco leaves has been studied by tissue printing and Northern (RNA) blot analysis. The mRNA was preferentially detected in and around the concentric necrotic rings caused by the viral infection. In addition, the accumulation pattern of the proteins was very similar although a basal signal was detected in nonsymptomatic tissue in uninoculated, systemically infected leaves. These results indicate a positive association of acidic chitinase gene expression with the necrotic tissue, where the virus is mainly localized, in the CLRV-tobacco interaction.

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